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[193103_s_at] Helicases conserved C-terminal domain

Description C04H5.6 /REP_DB=WormBase Gene ID /WP=CE15592 /TR=SW:O45244 /GB=CAB03845.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Helicases conserved C-terminal domain
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 57      4grp_highest_8_8 > 28      4grp_high_8_8 > 31      6grp_highest_nonorm > 90      6grp_total > 58      4grp_total > 32      6grp_highest_sub > Da      6grp_cgc > 38      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 206.6
(0.7)
P 		150.3
(-3)
P 		174.7
(-1.4)
P 		241.9
(3)
P 		92
B 150.1
(-3)
P 		180.5
(-0.3)
P 		216.5
(3)
P 		163.6
(-1.8)
P 		66
C 151.8
(-0.9)
P 		133.8
(-3)
P 		184.5
(3)
P 		149.6
(-1.1)
P 		51
avr 169.5
(-0.6) 		154.9
(-3) 		191.9
(3) 		185
(1.9) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 184
(0.1)
P 		134.7
(-2.1)
P 		114.7
(-3)
P 		172.2
(-0.4)
P 		184.6
(0.1)
P 		248.5
(3)
P 		134
B 146.7
(-1.4)
P 		142.9
(-1.5)
P 		173.3
(-0.3)
P 		107.8
(-3)
P 		169.3
(-0.4)
P 		250.8
(3)
P 		143
C 234.8
(3)
P 		154.9
(-2.1)
P 		191.1
(0.2)
P 		176
(-0.8)
P 		140.8
(-3)
P 		208.8
(1.3)
P 		94
avr 188.5
(-0.1) 		144.2
(-3) 		159.7
(-2) 		152
(-2.5) 		164.9
(-1.6) 		236
(3) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 75.2
(3)
P 		32.6
(-3)
P 		45.7
(-1.2)
P 		35.4
(-2.6)
P 		43
B 47.9
(2.1)
P 		44.6
(0.8)
P 		50.2
(3)
P 		34.9
(-3)
P 		15
C 50.2
(0.8)
P 		38.5
(-1)
P 		64.3
(3)
P 		25.2
(-3)
P 		39
avr 57.8
(3) 		38.6
(-1.4) 		53.4
(2) 		31.8
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 33.9
(-1.5)
P 		47.9
(0.9)
P 		25
(-3)
P 		43.7
(0.2)
P 		55.7
(2.2)
P 		60.5
(3)
P 		36
B 51.1
(-0.9)
P 		47.6
(-1.2)
P 		42.1
(-1.8)
P 		28.6
(-3)
P 		68.8
(0.7)
P 		93.5
(3)
P 		65
C 87
(3)
P 		47.3
(-1.6)
P 		48.6
(-1.5)
P 		35.4
(-3)
P 		45.6
(-1.8)
P 		69.9
(1)
P 		52
avr 57.3
(0.3) 		47.6
(-1.2) 		38.6
(-2.6) 		35.9
(-3) 		56.7
(0.2) 		74.6
(3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193103_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3679
Sequence Type Exemplar sequence
Representative Public ID C04H5.6 NCBI
Target Description C04H5.6 /REP_DB=WormBase Gene ID /WP=CE15592 /TR=SW:O45244 /GB=CAB03845.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Helicases conserved C-terminal domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064626(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064626 NCBI Caenorhabditis elegans Masculinisation Of Germline family member (mog-4) (mog-4) mRNA, complete cds. 11/11 None
C04H5.6 ENSEMBL cdna:known chromosome:CEL140:II:14552400:14561490:-1 gene:C04H5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14552561-14561484(-) 100.0 100.0
Public Domain and Genome References
Gene Title Helicases conserved C-terminal domain
Gene Symbol mog-4
Ensembl C04H5.6
Entrez Gene 175117 Entrez gene
SwissProt O45244 EMBL-EBI
WormBase CE15592 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260695_AT RNA helicase, putative at
CANINE_2:CFAAFFX.1606.1.S1_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 cfa
CANINE_2:CFAAFFX.1614.1.S1_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 cfa
DROSOPHILA_2:1635225_AT dm
DROSGENOME1:154338_AT dm
HG-U133_PLUS_2:203694_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HG-U133A:203694_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HG-U133A_2:203694_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HU35KSUBA:RC_AA086412_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HU35KSUBB:RC_AA034207_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HU35KSUBD:RC_N20004_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
U133_X3P:G13787201_3P_A_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HG-U95AV2:39896_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
HU35KSUBC:RC_D20460_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 hs
MG-U74AV2:100559_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MU11KSUBA:AA119035_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MU11KSUBB:MSA.35134.0_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MU11KSUBB:MSA.31679.0_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MOE430A:1423925_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MOUSE430A_2:1423925_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MOUSE430_2:1423925_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 mm
MU19KSUBB:TC31466_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16, mRNA (cDNA clone MGC:6487 IMAGE:2647184) mm
RG-U34B:RC_AA851883_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 rn
RAE230A:1399084_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 rn
RAT230_2:1399084_AT DEAH (Asp-Glu-Ala-His) box polypeptide 16 rn
YEAST_2:1772925_AT RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03845 Hypothetical protein C04H5.6 [Caenorhabditis elegans] emb|CAB03819.1| Hypothetical protein C04H5.6 [Caenorhabditis elegans] ref|NP_497027.1| Masculinisation Of Germline family member (mog-4) [Caenorhabditis elegans] gb|AAG01333.1| sex determining protein MOG-4 [Caenorhabditis elegans] sp|O45244|DHX16_CAEEL Probable pre-mRNA splicing factor ATP-dependent RNA helicase mog-4 (Sex determination protein mog-4) (Masculinization of germ line protein 4) 0.0
blast CAE73419 Hypothetical protein CBG20862 [Caenorhabditis briggsae] 0.0
blast AAF53766 CG10689-PA [Drosophila melanogaster] ref|NP_609946.1| CG10689-PA [Drosophila melanogaster] gb|AAL28878.1| LD25692p [Drosophila melanogaster] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007502 EMBL-EBI Helicase-associated region 5.0E-44
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.3E-14
Sequence
Target Sequence 
>C. ELEGANS:193103_S_AT
gattgcggcaatgctttcgtgtaatgcagctgtgttttatagaccgaaagcccaagtaat
tcacgcggattcggcacgtaaaggcttttggagtcccgccggagaccatatcaccctaat
gaatgtctacaataaatggcaagagtcgagcttctcgcaacgttggtgtgtggagaatta
tgtgcaacatcgaacaatgaaaagagcgcgagatgttcgagatcagttggttggactttt
ggaacgggtcgaaattgagacgaaatcttcaactgacacaatcaagattcgaaaagcaat
cacagccggatatttctataacgtttcaaaattggataatactggacactacaaaacggt
caagcacaagcacaccacacatccccatccaaattcgtgtcttttcgaggaaacgcctcg
atgggttgtctatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGCGGCAATGCTTTCGTGTAAT 379 433 2436 Antisense
AATGCAGCTGTGTTTTATAGACCGA 481 165 2458 Antisense
AAGCCCAAGTAATTCACGCGGATTC 586 149 2483 Antisense
CGCGGATTCGGCACGTAAAGGCTTT 623 221 2499 Antisense
CGGAGACCATATCACCCTAATGAAT 479 243 2535 Antisense
AATGGCAAGAGTCGAGCTTCTCGCA 143 163 2570 Antisense
GCTTCTCGCAACGTTGGTGTGTGGA 562 305 2585 Antisense
AAACGGTCAAGCACAAGCACACCAC 62 127 2789 Antisense
ACACATCCCCATCCAAATTCGTGTC 414 111 2812 Antisense
TCGTGTCTTTTCGAGGAAACGCCTC 474 605 2830 Antisense
GAAACGCCTCGATGGGTTGTCTATT 338 357 2845 Antisense
Sequence Source Affymetrix Proprietary Database