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[193100_s_at] glycosyl hydralase; sucrase-isomaltase

Description R05F9.12 /REP_DB=WormBase Gene ID /WP=CE04815 /TR=Q21750 /GB=AAA83174.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glycosyl hydralase; sucrase-isomaltase
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 41      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 16      6grp_total > 42      4grp_total > 62      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3967
1 2 3 4 range
A 4568.1
(3)
P 		2401.8
(-0.4)
P 		2997.8
(0.5)
P 		742.3
(-3)
P 		3826
B 3921.4
(3)
P 		2324.9
(-0)
P 		3247.2
(1.7)
P 		742.5
(-3)
P 		3179
C 4388.7
(3)
P 		2203
(-0.5)
P 		3221.6
(1.2)
P 		600.7
(-3)
P 		3788
avr 4292.7
(3) 		2309.9
(-0.3) 		3155.5
(1.1) 		695.2
(-3) 		3598

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9784
1 2 3 4 5 6 range
A 8741.2
(1.6)
P 		7178.3
(0.3)
P 		10447.1
(3)
P 		7653.9
(0.7)
P 		6039.7
(-0.7)
P 		3205.7
(-3)
P 		7241
B 7836.1
(-0.4)
P 		8333.7
(-0.1)
P 		12989.8
(3)
P 		7782.2
(-0.4)
P 		5410.6
(-2)
P 		3932.8
(-3)
P 		9057
C 6652.5
(-0.2)
P 		8336.2
(1.6)
P 		9640.3
(3)
P 		8284.9
(1.5)
P 		5485.3
(-1.4)
P 		4035.8
(-3)
P 		5605
avr 7743.3
(0.3) 		7949.4
(0.5) 		11025.7
(3) 		7907
(0.4) 		5645.2
(-1.4) 		3724.8
(-3) 		7301
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1562
1 2 3 4 range
A 1663.3
(3)
P 		521.4
(-1.4)
P 		783.7
(-0.4)
P 		108.7
(-3)
P 		1555
B 1250.5
(3)
P 		574.2
(-0.7)
P 		752.4
(0.3)
P 		158.2
(-3)
P 		1092
C 1452.8
(3)
P 		633.4
(-0.6)
P 		1122.8
(1.5)
P 		101.3
(-3)
P 		1352
avr 1455.5
(3) 		576.3
(-1) 		886.3
(0.4) 		122.7
(-3) 		1333

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2374
1 2 3 4 5 6 range
A 1611.4
(-0.2)
P 		2553
(3)
P 		2275.2
(2.1)
P 		1941.2
(0.9)
P 		1822.5
(0.5)
P 		780.5
(-3)
P 		1773
B 2726.6
(1.5)
P 		2773.9
(1.6)
P 		3154.3
(3)
P 		2061.2
(-0.9)
P 		2198.2
(-0.4)
P 		1466.5
(-3)
P 		1688
C 2465.8
(2.6)
P 		2543.6
(3)
P 		2453.6
(2.5)
P 		1666.1
(-1.4)
P 		1775
(-0.9)
P 		1351
(-3)
P 		1193
avr 2267.9
(1.5) 		2623.5
(3) 		2627.7
(3) 		1889.5
(-0.1) 		1931.9
(0.1) 		1199.3
(-3) 		1428
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193100_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5274
Sequence Type Exemplar sequence
Representative Public ID R05F9.12 NCBI
Target Description R05F9.12 /REP_DB=WormBase Gene ID /WP=CE04815 /TR=Q21750 /GB=AAA83174.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glycosyl hydralase; sucrase-isomaltase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062496(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062496 NCBI Caenorhabditis elegans R05F9.12 (R05F9.12) mRNA, complete cds. 11/11 None
R05F9.12.2 ENSEMBL cdna:known chromosome:CEL140:II:4914245:4925233:-1 gene:R05F9.12 11/11 None
R05F9.12.1 ENSEMBL cdna:known chromosome:CEL140:II:4914483:4925233:-1 gene:R05F9.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034939 3/11 Cross Hyb Matching Probes None
GENEFINDER00000034959 3/11 Cross Hyb Matching Probes None
SNAP00000043875 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4914591-4925178(-) 99.67 100.0
Public Domain and Genome References
Gene Title glycosyl hydralase; sucrase-isomaltase
Ensembl R05F9.12
Entrez Gene 173848 Entrez gene
SwissProt Q21750 EMBL-EBI
WormBase CE31976 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18297_AT alpha-glucosidase 1 (AGLU1) at
ATH1-121501:250336_AT alpha-glucosidase 1 (AGLU1) at
CANINE_2:CFAAFFX.6705.1.S1_AT similar to Maltase-glucoamylase, intestinal cfa
CANINE_2:CFAAFFX.6708.1.S1_AT similar to Maltase-glucoamylase, intestinal cfa
CANINE_2:CFAAFFX.6712.1.S1_AT similar to Maltase-glucoamylase, intestinal cfa
RAE230B:1379363_AT Maltase-glucoamylase (predicted) rn
RAT230_2:1379363_AT Maltase-glucoamylase (predicted) rn
RG-U34C:RC_AI233719_AT Maltase-glucoamylase (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494897 R05F9.12 [Caenorhabditis elegans] gb|AAA83174.3| Hypothetical protein R05F9.12 [Caenorhabditis elegans] 0.0
blast CAE59001 Hypothetical protein CBG02276 [Caenorhabditis briggsae] 0.0
blast CAE66278 Hypothetical protein CBG11523 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000519 EMBL-EBI P-type trefoil domain 8.7E-5
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193100_S_AT
gatacggtagcattatgtcgccaggatatgcaactgttccagcaccaactacttccagaa
ttccagtttttgtaagaggatacagtgttattccacgtcaaacaccatcaatcacaacaa
ctgcaactcgatcgaatccattcgaattattgattgcaccatgtccacttggaatgggag
aaggaacattgtattgggatgacggagagacaattgtcaatgatttcaattcttatgatt
atcatcaatttgatttcatgtataattcaactgcaactggtggaagtgttactataactc
actcgaaaaagagctccaaaatctcgcttccaactttggatatcattgaaattttcaact
atccaagtgctccaaacttccgatcattcacaatcaatggaaaattagtgaatgtcaatg
ttcagagatcaacatattctggaattaccaaaatattgtacatttccacagaagggctcg
taaatttgtcatctgctgattctattgtacttcagtggtcgaatgctggaaatagtatgt
tcgatatttcgtccgctagttcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACGGTAGCATTATGTCGCCAGG 509 423 2150 Antisense
GATATGCAACTGTTCCAGCACCAAC 427 425 2174 Antisense
CACCAACTACTTCCAGAATTCCAGT 160 201 2192 Antisense
GAGGATACAGTGTTATTCCACGTCA 539 403 2225 Antisense
CCACGTCAAACACCATCAATCACAA 330 275 2242 Antisense
ACAACAACTGCAACTCGATCGAATC 194 113 2263 Antisense
GATTGCACCATGTCCACTTGGAATG 608 433 2301 Antisense
AAAGAGCTCCAAAATCTCGCTTCCA 475 123 2457 Antisense
GTGCTCCAAACTTCCGATCATTCAC 166 479 2516 Antisense
GTACATTTCCACAGAAGGGCTCGTA 276 457 2607 Antisense
TTCGATATTTCGTCCGCTAGTTCAG 605 693 2689 Antisense
Sequence Source Affymetrix Proprietary Database