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[193089_at] GCN3 (yeast transcription activator)

Description ZK1098.4 /REP_DB=WormBase Gene ID /WP=CE00367 /TR=SW:P34604 /GB=CAA80132.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=GCN3 (yeast transcription activator)
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 63      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 75      6grp_total > 53      4grp_total > 58      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 736
1 2 3 4 range
A 905.9
(-2.4)
P 		1364.2
(3)
P 		1135.7
(0.3)
P 		853.9
(-3)
P 		510
B 1169
(0.5)
P 		1096.7
(-0.1)
P 		1479.3
(3)
P 		743.4
(-3)
P 		736
C 997.3
(-1.2)
P 		991.4
(-1.3)
P 		1278.5
(3)
P 		877.1
(-3)
P 		401
avr 1024.1
(-0.5) 		1150.8
(1.1) 		1297.8
(3) 		824.8
(-3) 		473

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 496
1 2 3 4 5 6 range
A 1036.4
(1.5)
P 		889
(-2.4)
P 		1092.4
(3)
P 		868.2
(-3)
P 		906.1
(-2)
P 		957.2
(-0.6)
P 		224
B 721
(-3)
P 		764.7
(-2.2)
P 		1054.2
(3)
P 		971.6
(1.5)
P 		908
(0.4)
P 		821.5
(-1.2)
P 		333
C 849
(-2.2)
P 		791.3
(-3)
P 		1217.4
(3)
P 		917.8
(-1.2)
P 		957.6
(-0.7)
P 		993
(-0.2)
P 		426
avr 868.8
(-1.9) 		815
(-3) 		1121.3
(3) 		919.2
(-1) 		923.9
(-0.9) 		923.9
(-0.9) 		306
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 321
1 2 3 4 range
A 329.8
(3)
P 		296.1
(2)
P 		296.9
(2)
P 		125
(-3)
P 		205
B 372.8
(3)
P 		270.8
(0.1)
P 		342.8
(2.2)
P 		158.4
(-3)
P 		214
C 330.1
(0.7)
P 		285
(-0.2)
P 		445.6
(3)
P 		147.9
(-3)
P 		298
avr 344.2
(2.5) 		284
(0.9) 		361.8
(3) 		143.8
(-3) 		218

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 191.1
(-3)
P 		316.2
(3)
P 		237.9
(-0.8)
P 		220.2
(-1.6)
P 		273.4
(0.9)
P 		233
(-1)
P 		125
B 250.9
(-3)
P 		254.5
(-2.8)
P 		256
(-2.7)
P 		257.4
(-2.7)
P 		368.9
(3)
P 		306.3
(-0.2)
P 		118
C 314.7
(2.3)
P 		241.5
(-0.7)
P 		309.8
(2.1)
P 		184.6
(-3)
P 		309.9
(2.1)
P 		332.4
(3)
P 		148
avr 252.2
(-1) 		270.7
(0.1) 		267.9
(-0.1) 		220.7
(-3) 		317.4
(3) 		290.6
(1.3) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193089_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6804
Sequence Type Exemplar sequence
Representative Public ID ZK1098.4 NCBI
Target Description ZK1098.4 /REP_DB=WormBase Gene ID /WP=CE00367 /TR=SW:P34604 /GB=CAA80132.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=GCN3 (yeast transcription activator)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066705(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066705 NCBI Caenorhabditis elegans ZK1098.4 (ZK1098.4) mRNA, complete cds. 11/11 None
SNAP00000005016 ENSEMBL cdna:SNAP chromosome:CEL140:III:9543038:9544177:1 11/11 None
GENEFINDER00000005022 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9543038:9544177:1 11/11 None
ZK1098.4 ENSEMBL cdna:known chromosome:CEL140:III:9543009:9544304:1 gene:ZK1098.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9543033-9544173(+) 100.0 100.0
Public Domain and Genome References
Gene Title GCN3 (yeast transcription activator)
Ensembl ZK1098.4
Entrez Gene 176348 Entrez gene
SwissProt P34604 EMBL-EBI
WormBase CE00367 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260428_AT eukaryotic translation initiation factor 2B family protein / eIF-2B family protein at
CANINE_2:CFA.19262.1.S1_AT similar to Translation initiation factor eIF-2B alpha subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFA.21102.1.S1_S_AT similar to Translation initiation factor eIF-2B alpha subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFAAFFX.12030.1.S1_AT similar to Translation initiation factor eIF-2B alpha subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFAAFFX.12033.1.S1_AT similar to Translation initiation factor eIF-2B alpha subunit (eIF-2B GDP-GTP exchange factor) cfa
DROSOPHILA_2:1639421_AT dm
DROSGENOME1:153369_AT dm
CHICKEN:GGA.6305.1.S1_AT similar to Translation initiation factor eIF-2B alpha subunit (eIF-2B GDP-GTP exchange factor) gga
HU35KSUBC:RC_AA609559_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HU35KSUBD:RC_H72081_S_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HUGENEFL:X95648_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-U133A:201632_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-U133_PLUS_2:201632_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-FOCUS:201632_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-U133A_2:201632_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
U133_X3P:G4503502_3P_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-U95AV2:37665_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-U95B:44888_AT eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
HG-U95E:85679_AT Eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa hs
MU11KSUBA:AA266894_S_AT eukaryotic translation initiation factor 2B, subunit 1 (alpha) mm
MG-U74AV2:94462_AT eukaryotic translation initiation factor 2B, subunit 1 (alpha) mm
MOE430A:1460730_AT eukaryotic translation initiation factor 2B, subunit 1 (alpha) mm
MOUSE430A_2:1460730_AT eukaryotic translation initiation factor 2B, subunit 1 (alpha) mm
MOUSE430_2:1460730_AT eukaryotic translation initiation factor 2B, subunit 1 (alpha) mm
RG-U34A:RC_AI031019_AT eukaryotic translation initiation factor 2B, subunit 1 alpha rn
RG-U34A:RC_AI031019_G_AT eukaryotic translation initiation factor 2B, subunit 1 alpha rn
RG-U34B:RC_AI007997_S_AT eukaryotic translation initiation factor 2B, subunit 1 alpha rn
RAE230A:1370311_AT eukaryotic translation initiation factor 2B, subunit 1 alpha rn
RAT230_2:1370311_AT eukaryotic translation initiation factor 2B, subunit 1 alpha rn
YEAST_2:1772352_AT Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6413 translational initiation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5851 eukaryotic translation initiation factor 2B complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA80132 Hypothetical protein ZK1098.4 [Caenorhabditis elegans] ref|NP_499106.1| ZK1098.4 [Caenorhabditis elegans] sp|P34604|EI2BA_CAEEL Putative translation initiation factor eIF-2B alpha subunit (eIF-2B GDP-GTP exchange factor) 1.0E-169
blast CAE65184 Hypothetical protein CBG10057 [Caenorhabditis briggsae] 1.0E-160
blast NP_001017286 hypothetical protein LOC550040 [Xenopus tropicalis] 1.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR000649 EMBL-EBI Initiation factor 2B 6.1E-121
Sequence
Target Sequence 
>C. ELEGANS:193089_AT
tatcgccaacgagcacgctcatttataaagaatgtcagaggttctcgagccaaaatttcc
aagtgcgctcgtctatttttcactcatcatatgaatattctgacacactcatacagcaaa
gtggttctggaaactattcttgatgctcacaaaagtggatatcaccttcatgtctgggtc
accgaatcgcaaccggatgcttcaggaaaactagtgtttgaagaattgaaaaagaatgga
gttccaactactttagttctagatagctgtgtgggatatgtcatggaaagaattcaagca
gttctagttggcgctgaaggagtcatggaaaccggtggaatcatcaataaaattggaact
gttaatgtttgtatcatcgcaaaatcacggcatgttcccgtctatgtttgcgctgaaact
atcaagtttgtacgggagttcccactcaaccaggccgatattccccaagaattcaaatat
cgaacatcagtaattgagagaaacaacctagaattggagcatcccgatgtggattacacc
gcacccgaatttctgacactgatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCGCCAACGAGCACGCTCATTTA 47 659 301 Antisense
AGAGGTTCTCGAGCCAAAATTTCCA 418 67 337 Antisense
TCCAAGTGCGCTCGTCTATTTTTCA 668 587 358 Antisense
ATATCACCTTCATGTCTGGGTCACC 282 19 459 Antisense
ACCGAATCGCAACCGGATGCTTCAG 33 89 481 Antisense
AAGCAGTTCTAGTTGGCGCTGAAGG 259 151 596 Antisense
AATCACGGCATGTTCCCGTCTATGT 527 171 683 Antisense
TTGTACGGGAGTTCCCACTCAACCA 368 703 728 Antisense
CAGGCCGATATTCCCCAAGAATTCA 615 145 751 Antisense
ATCCCGATGTGGATTACACCGCACC 172 39 821 Antisense
GCACCCGAATTTCTGACACTGATCA 140 315 841 Antisense
Sequence Source Affymetrix Proprietary Database