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[193060_at] ribonuclease H magnesium binding domain

Description ZK938.7 /REP_DB=WormBase Gene ID /WP=CE03839 /TR=Q23676 /GB=CAA90146.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ribonuclease H magnesium binding domain
Cluster 6grp_total > 53      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 8.5
(-2.6)
A 		15.9
(-1.5)
A 		5.5
(-3)
A 		48.5
(3)
P 		43
B 13.5
(-1.9)
A 		57.6
(3)
A 		45.9
(1.7)
P 		3.7
(-3)
A 		54
C 30.6
(-0.7)
A 		7
(-3)
A 		9.4
(-2.8)
A 		68.8
(3)
P 		62
avr 17.5
(-3) 		26.8
(-0.6) 		20.3
(-2.3) 		40.3
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 7.4
(-2.4)
A 		5.8
(-2.7)
A 		37.8
(3)
P 		23.3
(0.4)
A 		4.3
(-3)
A 		5
(-2.9)
A 		34
B 6.3
(-3)
A 		11.6
(-1.4)
A 		22.4
(1.8)
A 		17.3
(0.3)
A 		26.6
(3)
A 		19.1
(0.8)
A 		20
C 21.1
(-0.3)
A 		26.8
(0.6)
A 		43.8
(3)
A 		3.3
(-2.8)
A 		2.1
(-3)
A 		21.2
(-0.3)
A 		42
avr 11.6
(-2.8) 		14.7
(-2.1) 		34.7
(3) 		14.6
(-2.1) 		11
(-3) 		15.1
(-2) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 3.1
(-1.2)
A 		3.4
(-0.9)
A 		1.4
(-3)
A 		7.1
(3)
P 		6
B 4.3
(-1.4)
A 		14.2
(3)
A 		10.6
(1.4)
P 		0.8
(-3)
A 		13
C 10.1
(2.1)
A 		2
(-3)
A 		3.3
(-2.2)
A 		11.6
(3)
P 		10
avr 5.8
(0) 		6.5
(3) 		5.1
(-3) 		6.5
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.4
(-2.8)
A 		2.1
(-2.2)
A 		8.2
(3)
P 		5.9
(1)
A 		1.3
(-2.9)
A 		1.2
(-3)
A 		7
B 2.2
(-3)
A 		3.9
(-1.8)
A 		5.4
(-0.8)
A 		4.6
(-1.3)
A 		10.8
(3)
A 		7.1
(0.4)
A 		9
C 7.8
(1.1)
A 		8.2
(1.3)
A 		11.2
(3)
A 		0.7
(-3)
A 		0.7
(-3)
A 		7.1
(0.7)
A 		11
avr 3.8
(-2.9) 		4.7
(-1.7) 		8.3
(3) 		3.7
(-3) 		4.3
(-2.2) 		5.1
(-1.2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193060_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2767
Sequence Type Exemplar sequence
Representative Public ID ZK938.7 NCBI
Target Description ZK938.7 /REP_DB=WormBase Gene ID /WP=CE03839 /TR=Q23676 /GB=CAA90146.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ribonuclease H magnesium binding domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063720(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063720 NCBI Caenorhabditis elegans ZK938.7 (ZK938.7) mRNA, complete cds. 11/11 None
ZK938.7 ENSEMBL cdna:known chromosome:CEL140:II:9839680:9841932:-1 gene:ZK938.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000044677 8/11 Cross Hyb Matching Probes None
GENEFINDER00000044690 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9839676-9841929(-) 100.0 100.0
Public Domain and Genome References
Gene Title ribonuclease H magnesium binding domain
Ensembl ZK938.7
Entrez Gene 191471 Entrez gene
SwissProt Q23676 EMBL-EBI
WormBase CE03839 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4523 ribonuclease H activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90146 Hypothetical protein ZK938.7 [Caenorhabditis elegans] ref|NP_496121.1| ZK938.7 [Caenorhabditis elegans] 0.0
blast CAA90142 Hypothetical protein ZK938.2 [Caenorhabditis elegans] ref|NP_496120.1| ZK938.2 [Caenorhabditis elegans] 1.0E-21
blast NP_493774 T04B8.3 [Caenorhabditis elegans] 1.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR002156 EMBL-EBI RNase H 2.0E-6
Pfam IPR000698 EMBL-EBI Arrestin 3.6E-20
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496121.1 1 183-202
ZK938.7 1 183-202
Sequence
Target Sequence 
>C. ELEGANS:193060_AT
gaaatccacatcaatctgcaaacagagacagagtaccagagcagtttggatttcatagat
atcagaaattgtgtattatatcttatttgtacatttccaagcctcgaaatcactcttgac
aaagcatcatattgccctggggactatgtaactgccacagttttagtagataatcagaaa
ccttttgatgcaagatctgtgatgcttacgattcgaggaaccttctgttatgaaacatac
ttcaaacggtcagcgatcgtatggacttccaaggacggaaagaacaaactccctgtagag
ctgctagaaaaatcattcgaatttcaaatacctacgggtagccgtttaacgtacaaggct
gggcacatcaagtatacgatgagcttggaagttgatcgaccatggaaaactaacttgaaa
gttaaaaaagaagtgattgtagcgcgtaaacttgatgttgatgaagtttacttcactgag
aacaaaactgtctcgaataaacctgcaatcggagtttttcaaatgggcagctcaacttca
cggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCCACATCAATCTGCAAACAG 74 363 637 Antisense
TATTTGTACATTTCCAAGCCTCGAA 50 663 720 Antisense
AAGCCTCGAAATCACTCTTGACAAA 173 149 735 Antisense
AAAGCATCATATTGCCCTGGGGACT 401 123 757 Antisense
CTGGGGACTATGTAACTGCCACAGT 180 241 773 Antisense
GAAACATACTTCAAACGGTCAGCGA 410 355 868 Antisense
GGTCAGCGATCGTATGGACTTCCAA 231 503 884 Antisense
GAACAAACTCCCTGTAGAGCTGCTA 593 351 918 Antisense
AAGGCTGGGCACATCAAGTATACGA 39 161 991 Antisense
AACTGTCTCGAATAAACCTGCAATC 280 143 1122 Antisense
CAAATGGGCAGCTCAACTTCACGGT 224 191 1156 Antisense
Sequence Source Affymetrix Proprietary Database