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[193058_at]

Description C29E6.1 /REP_DB=WormBase Gene ID /WP=CE05332 /GEN=let-653 /TR=Q27394 /GB=CAA96602.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=LET-653 mucin like protein
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 0      6grp_highest_nonorm > 60      6grp_total > 09      4grp_total > 50      6grp_cgc > 10      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 772
1 2 3 4 range
A 135
(-2.2)
P 		707.7
(2.4)
P 		777.1
(3)
P 		30.8
(-3)
A 		746
B 144.7
(-2)
P 		789.4
(3)
P 		523.8
(0.9)
P 		17.5
(-3)
A 		772
C 111.9
(-2.6)
P 		697.9
(3)
P 		689.8
(2.9)
P 		69.5
(-3)
A 		628
avr 130.5
(-2.2) 		731.7
(3) 		663.6
(2.4) 		39.3
(-3) 		692

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 706
1 2 3 4 5 6 range
A 594.6
(3)
P 		335.4
(-0.4)
P 		326.3
(-0.5)
P 		524.6
(2.1)
P 		266.7
(-1.3)
P 		137.3
(-3)
P 		457
B 749.9
(3)
P 		343.4
(-1.2)
P 		359.1
(-1.1)
P 		491.7
(0.3)
P 		282.4
(-1.9)
P 		171.9
(-3)
P 		578
C 821.4
(3)
P 		392
(-0.6)
P 		397.1
(-0.6)
P 		459.6
(-0.1)
P 		275.2
(-1.6)
P 		115.4
(-3)
P 		706
avr 722
(3) 		356.9
(-0.8) 		360.8
(-0.7) 		492
(0.6) 		274.8
(-1.6) 		141.5
(-3) 		581
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 237
1 2 3 4 range
A 49.2
(-1.6)
P 		153.6
(1.5)
P 		203.1
(3)
P 		4.5
(-3)
A 		199
B 46.1
(-1.7)
P 		195
(3)
P 		121.4
(0.7)
P 		3.7
(-3)
A 		191
C 37
(-2.3)
P 		200.6
(2)
P 		240.4
(3)
P 		11.7
(-3)
A 		229
avr 44.1
(-1.8) 		183.1
(2.8) 		188.3
(3) 		6.6
(-3) 		182

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 271
1 2 3 4 5 6 range
A 109.6
(1.6)
P 		119.3
(2.2)
P 		71.1
(-0.7)
P 		133.1
(3)
P 		80.5
(-0.2)
P 		33.4
(-3)
P 		100
B 260.9
(3)
P 		114.3
(-1.5)
P 		87.2
(-2.3)
P 		130.2
(-1)
P 		114.7
(-1.5)
P 		64.1
(-3)
P 		197
C 304.5
(3)
P 		119.6
(-1.2)
P 		101.1
(-1.6)
P 		92.4
(-1.8)
P 		89
(-1.9)
P 		38.6
(-3)
P 		266
avr 225
(3) 		117.7
(-0.6) 		86.5
(-1.6) 		118.6
(-0.6) 		94.7
(-1.4) 		45.4
(-3) 		180
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193058_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8549
Sequence Type Exemplar sequence
Representative Public ID C29E6.1 NCBI
Target Description C29E6.1 /REP_DB=WormBase Gene ID /WP=CE05332 /GEN=let-653 /TR=Q27394 /GB=CAA96602.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=LET-653 mucin like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026166(11), NM_001026165(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026166 NCBI Caenorhabditis elegans LEThal family member (let-653) (let-653) mRNA, complete cds. 11/11 None
NM_001026165 NCBI Caenorhabditis elegans LEThal family member (let-653) (let-653) mRNA, complete cds. 11/11 None
SNAP00000002878 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11885588:11890862:1 11/11 None
GENEFINDER00000002885 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11885588:11890862:1 11/11 None
C29E6.1b.2 ENSEMBL cdna:known chromosome:CEL140:IV:11885500:11891037:1 gene:C29E6.1 11/11 None
C29E6.1b.1 ENSEMBL cdna:known chromosome:CEL140:IV:11885585:11891052:1 gene:C29E6.1 11/11 None
C29E6.1a ENSEMBL cdna:known chromosome:CEL140:IV:11885588:11890862:1 gene:C29E6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11885593-11890868(+) 91.5 100.0
Public Domain and Genome References
Ensembl C29E6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAH60755 Hypothetical protein C29E6.1b [Caenorhabditis elegans] ref|NP_001021337.1| LEThal family member (let-653) [Caenorhabditis elegans] 0.0
blast CAA96602 Hypothetical protein C29E6.1a [Caenorhabditis elegans] ref|NP_001021336.1| LEThal family member (let-653) [Caenorhabditis elegans] emb|CAA62505.1| let-653 [Caenorhabditis elegans] 0.0
blast CAE62140 Hypothetical protein CBG06185 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003014 EMBL-EBI N/apple PAN 3.1E-24
Sequence
Target Sequence 
>C. ELEGANS:193058_AT
aaaaatcagtctatcattccgtccacactatccaattccaccggagtacgtagacgttcg
tggttgccaagttgagccaattgatccaaaatatgaatgggagcacgagccactgttcat
cataagagatggatgtcctgctgacggtgttggtctcgtctgcccaccaacacattctga
gtttggagcaaaagtttccgttgaggcattcagatatcaaacaactggacaagtacagta
ctcctgtctcgtgagaatatgtccatttgctccgtgtccgaagaacacatgcgacgacgt
ggaaggctgtgacagtagctacatgcatcgatacaggcgagaattatcacttgaagacat
caaaaaagcattggaagcaaacccagaacttgcttctcagttcggaatttcaccgtccgc
gtttgcaagaaatccgtcgaaatctaaaaatttcacaagcgttgttgaagaacaacaaag
aatcgcactcggcggagattatcttgttcggcgtcgatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATCAGTCTATCATTCCGTCCA 648 129 1518 Antisense
ACACTATCCAATTCCACCGGAGTAC 675 107 1542 Antisense
GTACGTAGACGTTCGTGGTTGCCAA 96 459 1563 Antisense
TGCTGACGGTGTTGGTCTCGTCTGC 171 583 1656 Antisense
TGCCCACCAACACATTCTGAGTTTG 626 583 1678 Antisense
CAAGTACAGTACTCCTGTCTCGTGA 702 183 1747 Antisense
GAGAATATGTCCATTTGCTCCGTGT 405 391 1770 Antisense
GCGACGACGTGGAAGGCTGTGACAG 170 289 1808 Antisense
TCAGTTCGGAATTTCACCGTCCGCG 616 621 1914 Antisense
GTCCGCGTTTGCAAGAAATCCGTCG 9 477 1932 Antisense
GAGATTATCTTGTTCGGCGTCGATT 186 397 2012 Antisense
Sequence Source Affymetrix Proprietary Database