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[193054_s_at]

Description Y43C5A.6 /REP_DB=WormBase Gene ID /WP=CE25285 /GEN=rad-51 /TR=O44246 /GB=CAB61038.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Helix-hairpin-helix motif.
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 96      4grp_highest_8_8 > 03      4grp_high_8_8 > 37      6grp_highest_nonorm > 95      6grp_total > 97      4grp_total > 42      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 481
1 2 3 4 range
A 330.6
(-3)
P 		784.9
(3)
P 		554.3
(-0)
P 		484.1
(-1)
P 		454
B 303.8
(-3)
P 		779.2
(3)
P 		621.9
(1)
P 		449.1
(-1.2)
P 		475
C 506.1
(-2.5)
P 		673.4
(3)
P 		645
(2.1)
P 		489.7
(-3)
P 		184
avr 380.2
(-3) 		745.8
(3) 		607.1
(0.7) 		474.3
(-1.5) 		366

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 911
1 2 3 4 5 6 range
A 370.3
(-3)
P 		371.5
(-3)
P 		449.3
(-2.5)
P 		600.9
(-1.4)
P 		763.7
(-0.3)
P 		1244
(3)
P 		874
B 378.7
(-2.7)
P 		333.5
(-3)
P 		410
(-2.5)
P 		612.7
(-1)
P 		855.2
(0.7)
P 		1182.1
(3)
P 		849
C 416
(-2.5)
P 		358.7
(-3)
P 		618.4
(-0.6)
P 		558.1
(-1.2)
P 		812.5
(1.1)
P 		1016.4
(3)
P 		658
avr 388.3
(-2.7) 		354.6
(-3) 		492.6
(-2) 		590.6
(-1.2) 		810.5
(0.4) 		1147.5
(3) 		793
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 120.4
(-0)
P 		170.4
(3)
P 		144.9
(1.5)
P 		70.9
(-3)
P 		100
B 96.9
(-2.9)
P 		192.5
(3)
P 		144.1
(-0)
P 		95.7
(-3)
P 		97
C 167.5
(0.6)
P 		193.6
(1.7)
P 		224.8
(3)
P 		82.6
(-3)
P 		142
avr 128.3
(-0.4) 		185.5
(3) 		171.3
(2.2) 		83.1
(-3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 68.3
(-3)
P 		132.1
(-1.4)
P 		97.8
(-2.2)
P 		152.4
(-0.8)
P 		230.4
(1.1)
P 		302.9
(3)
P 		235
B 131.8
(-2.4)
P 		111
(-2.8)
P 		99.6
(-3)
P 		162.3
(-1.9)
P 		347.5
(1.4)
P 		440.8
(3)
P 		341
C 154.2
(-1.8)
P 		109.4
(-3)
P 		157.4
(-1.8)
P 		112.2
(-2.9)
P 		262.9
(1)
P 		340.2
(3)
P 		231
avr 118.1
(-3) 		117.5
(-3) 		118.3
(-3) 		142.3
(-2.4) 		280.3
(1) 		361.3
(3) 		244
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193054_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9067
Sequence Type Exemplar sequence
Representative Public ID Y43C5A.6 NCBI
Target Description Y43C5A.6 /REP_DB=WormBase Gene ID /WP=CE25285 /GEN=rad-51 /TR=O44246 /GB=CAB61038.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Helix-hairpin-helix motif.
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028295(11), NM_001028294(11), AF061201(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028295 NCBI Caenorhabditis elegans RADiation sensitivity abnormal/yeast RAD-related family member (rad-51) (rad-51) mRNA, complete cds. 11/11 None
NM_001028294 NCBI Caenorhabditis elegans RADiation sensitivity abnormal/yeast RAD-related family member (rad-51) (rad-51) mRNA, complete cds. 11/11 None
AF061201 NCBI Caenorhabditis elegans RAD51 short isoform (rad51) mRNA, complete cds. 11/11 None
SNAP00000044405 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10281947:10283976:-1 11/11 None
GENEFINDER00000044406 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10281947:10283985:-1 11/11 None
Y43C5A.6b ENSEMBL cdna:known chromosome:CEL140:IV:10281657:10283985:-1 gene:Y43C5A.6 11/11 None
Y43C5A.6a ENSEMBL cdna:known chromosome:CEL140:IV:10281689:10284215:-1 gene:Y43C5A.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10281952-10283982(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y43C5A.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61038 Hypothetical protein Y43C5A.6a [Caenorhabditis elegans] ref|NP_001023465.1| RADiation sensitivity abnormal/yeast RAD-related family member (rad-51) [Caenorhabditis elegans] 0.0
blast CAE47473 Hypothetical protein Y43C5A.6b [Caenorhabditis elegans] ref|NP_001023466.1| RADiation sensitivity abnormal/yeast RAD-related family member (rad-51) [Caenorhabditis elegans] gb|AAD10194.1| RAD51 short isoform [Caenorhabditis elegans] dbj|BAA24982.1| RecA/Rad51/Dmc1-like protein [Caenorhabditis elegans] 0.0
blast T26822 hypothetical protein Y43C5A.6 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000445 EMBL-EBI Helix-hairpin-helix motif 1.0E-6
Pfam IPR000445 EMBL-EBI Helix-hairpin-helix motif 1.0E-6
Pfam IPR000445 EMBL-EBI Helix-hairpin-helix motif 1.0E-6
Sequence
Target Sequence 
>C. ELEGANS:193054_S_AT
aaaactcagctttgccacagtctggcagtcttgtgtcaattgccgattgatatgggaggt
ggtgagggaaaatgtatgtatattgataccaatgccacttttcgacccgaacgaattatt
gctatcgctcagagatacaatatggacagtgctcatgtactcgagaatatcgctgttgca
cgtgcatacaactcagaacatttgatggctttgattattcgagcaggagcaatgatgtcc
gagagtcgttatgctgttgtgattgttgactgtgcaactgctcatttccgtaacgaatac
actggaagaggagatctagcggaacgtcagatgaagctctctgcttttctgaaatgcctt
gccaagcttgccgatgaatatggtgtcgctgtaattattaccaatcaagttgtagcacaa
gtcgacggaggagcctcgatgttccaggctgatgctaaaaagcccattggaggtcacatc
atcgctcacatgtctactaccagattgtaccttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAACTCAGCTTTGCCACAGTCTGG 295 131 457 Antisense
CAGTCTGGCAGTCTTGTGTCAATTG 610 207 474 Antisense
TTGATACCAATGCCACTTTTCGACC 683 703 539 Antisense
GAGAATATCGCTGTTGCACGTGCAT 390 391 619 Antisense
GTCCGAGAGTCGTTATGCTGTTGTG 132 477 693 Antisense
TGACTGTGCAACTGCTCATTTCCGT 447 569 723 Antisense
AAGCTCTCTGCTTTTCTGAAATGCC 62 151 790 Antisense
TGAAATGCCTTGCCAAGCTTGCCGA 182 573 806 Antisense
AAAGCCCATTGGAGGTCACATCATC 382 125 915 Antisense
TCACATCATCGCTCACATGTCTACT 18 629 930 Antisense
GTCTACTACCAGATTGTACCTTCGA 708 469 948 Antisense
Sequence Source Affymetrix Proprietary Database