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[193039_s_at] Proteasome A-type and B-type

Description K05C4.1 /REP_DB=WormBase Gene ID /WP=CE19969 /GEN=pbs-5 /TR=Q9XUV0 /GB=CAB04567.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Proteasome A-type and B-type
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 0      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 46      6grp_total > 09      4grp_total > 57      6grp_cgc > 10      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3265
1 2 3 4 range
A 2903.4
(-2.7)
P 		4962.9
(1.4)
P 		5744
(3)
P 		2732
(-3)
P 		3012
B 3492.7
(-1.9)
P 		4114.4
(0.1)
P 		5020.4
(3)
P 		3162.5
(-3)
P 		1858
C 2479.2
(-3)
P 		4035.9
(2.2)
P 		4268
(3)
P 		2618.5
(-2.5)
P 		1789
avr 2958.4
(-2.7) 		4371.1
(1.2) 		5010.8
(3) 		2837.7
(-3) 		2173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1808
1 2 3 4 5 6 range
A 3835
(3)
P 		2598.6
(-2.3)
P 		2434.3
(-3)
P 		3187.7
(0.2)
P 		2803.2
(-1.4)
P 		2559.6
(-2.5)
P 		1401
B 2743.3
(3)
P 		2619.1
(1.8)
P 		2740
(3)
P 		2436.6
(-0)
P 		2537.5
(1)
P 		2133.8
(-3)
P 		610
C 2847.4
(2)
P 		2558.1
(0.3)
P 		2979.3
(2.9)
P 		3003.3
(3)
P 		2728.8
(1.3)
P 		2026.8
(-3)
P 		977
avr 3141.9
(3) 		2591.9
(-0.7) 		2717.9
(0.2) 		2875.9
(1.2) 		2689.8
(-0) 		2240.1
(-3) 		902
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1102
1 2 3 4 range
A 1057.1
(0.6)
P 		1077.3
(0.7)
P 		1501.6
(3)
P 		399.9
(-3)
P 		1102
B 1113.8
(2.4)
P 		1016.2
(1.2)
P 		1163.3
(3)
P 		673.9
(-3)
P 		489
C 820.7
(-0.8)
P 		1160.4
(1.1)
P 		1487.5
(3)
P 		441.5
(-3)
P 		1046
avr 997.2
(0.4) 		1084.6
(1) 		1384.1
(3) 		505.1
(-3) 		879

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 525
1 2 3 4 5 6 range
A 706.9
(-0.3)
P 		924.2
(3)
P 		530.1
(-3)
P 		808.5
(1.2)
P 		845.9
(1.8)
P 		623.2
(-1.6)
P 		394
B 954.5
(1.8)
P 		871.8
(0.5)
P 		665.3
(-2.7)
P 		645.4
(-3)
P 		1030.9
(3)
P 		795.7
(-0.7)
P 		386
C 1055.4
(3)
P 		780.5
(-0.7)
P 		758.3
(-0.9)
P 		604
(-3)
P 		883
(0.7)
P 		678.5
(-2)
P 		451
avr 905.6
(2.7) 		858.8
(1.6) 		651.2
(-3) 		686
(-2.2) 		919.9
(3) 		699.1
(-1.9) 		269
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193039_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1658
Sequence Type Exemplar sequence
Representative Public ID K05C4.1 NCBI
Target Description K05C4.1 /REP_DB=WormBase Gene ID /WP=CE19969 /GEN=pbs-5 /TR=Q9XUV0 /GB=CAB04567.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Proteasome A-type and B-type
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061157(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061157 NCBI Caenorhabditis elegans Proteasome Beta Subunit family member (pbs-5) (pbs-5) mRNA, complete cds. 11/11 None
SNAP00000018534 ENSEMBL cdna:SNAP chromosome:CEL140:I:14727474:14728580:-1 11/11 None
GENEFINDER00000018547 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14727474:14728580:-1 11/11 None
K05C4.1.2 ENSEMBL cdna:known chromosome:CEL140:I:14727302:14728597:-1 gene:K05C4.1 11/11 None
K05C4.1.1 ENSEMBL cdna:known chromosome:CEL140:I:14727302:14728595:-1 gene:K05C4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14727404-14728511(-) 99.88 99.88
Public Domain and Genome References
Gene Title Proteasome A-type and B-type
Gene Symbol pbs-5
Ensembl K05C4.1
Entrez Gene 173334 Entrez gene
SwissProt Q9XUV0 EMBL-EBI
WormBase CE19969 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262781_S_AT 20S proteasome beta subunit E, putative at
CANINE:1589711_S_AT similar to proteasome beta 5 subunit cfa
CANINE_2:CFA.6208.1.A1_S_AT similar to proteasome beta 5 subunit cfa
CANINE_2:CFAAFFX.17609.1.S1_S_AT similar to proteasome beta 5 subunit cfa
CANINE_2:CFAAFFX.17614.1.S1_AT similar to proteasome beta 5 subunit cfa
DROSOPHILA_2:1636559_S_AT dm
DROSGENOME1:153637_AT dm
HG-U133_PLUS_2:208799_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
HG-FOCUS:208799_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
HG-U133A_2:208799_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
HG-U133A:208799_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
HUGENEFL:X95586_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
U133_X3P:G13278740_3P_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
HG-U95AV2:37666_AT proteasome (prosome, macropain) subunit, beta type, 5 hs
MG-U74AV2:160559_AT proteasome (prosome, macropain) subunit, beta type 5 mm
MG-U74AV2:101558_S_AT proteasome (prosome, macropain) subunit, beta type 5 mm
MG-U74BV2:109821_F_AT proteasome (prosome, macropain) subunit, beta type 5 mm
MOE430A:1415676_A_AT proteasome (prosome, macropain) subunit, beta type 5 mm
MOE430B:1415676_A_AT proteasome (prosome, macropain) subunit, beta type 5 mm
MOUSE430A_2:1415676_A_AT proteasome (prosome, macropain) subunit, beta type 5 mm
MOUSE430_2:1415676_A_AT proteasome (prosome, macropain) subunit, beta type 5 mm
RG-U34A:D45247_G_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RG-U34A:D45247_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RG-U34A:RC_AA891226_S_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RG-U34B:RC_AI011563_S_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RAE230A:1371348_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RAT230_2:1371348_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RAE230B:1385531_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RAT230_2:1385531_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RAE230B:1385532_X_AT proteasome (prosome, macropain) subunit, beta type 5 rn
RAT230_2:1385532_X_AT proteasome (prosome, macropain) subunit, beta type 5 rn
YEAST_2:1773311_AT 20S proteasome beta-type subunit, responsible for the chymotryptic activity of the proteasome Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
9401 phosphoenolpyruvate-dependent sugar phosphotransferase system inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5839 proteasome core complex (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4175 endopeptidase activity inferred from electronic annotation QuickGO AmiGO
5351 sugar porter activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04567 Hypothetical protein K05C4.1 [Caenorhabditis elegans] ref|NP_493558.1| Proteasome Beta Subunit family member (pbs-5) [Caenorhabditis elegans] 1.0E-164
blast CAE72557 Hypothetical protein CBG19741 [Caenorhabditis briggsae] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 3.6E-59
Sequence
Target Sequence 
>C. ELEGANS:193039_S_AT
tcgattcacgagcctccagtggtgaatacatctcatccaagtcggtcatgaagatcttgg
acatcggagatcgtatggttgccacgatggctggaggagctgctgactgtcagttctgga
cccgaattgtcgccaagtactgcactttgtacgagctccgcgagaagacctcaatcaccg
tgagcgccgcgagcaagtactttgcaaatacgctctatggatacagaggacaaggattgt
cagttggatcgatggtggccggatacgataagaagggaccacaaatcttcaaagtcgact
cggaaggagatcgttgccagcttaaagtgtgctcagttggctcaggatctctgaatgcct
acggtatcctcgataatcactacaagccaaagatgacggacgacgaggccagaaagctcg
gcctgcgtgcgatcatgcacgccacttatcgggattctggatccggaggcgtctgcaatt
tgtgccacatcacgccaaccgaaaagattcgtcttccaccaatggacgtgagcaagctgt
ggtacgagtttgccgatgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGATTCACGAGCCTCCAGTGGTGA 636 603 272 Antisense
GAATACATCTCATCCAAGTCGGTCA 232 327 295 Antisense
TGACTGTCAGTTCTGGACCCGAATT 372 569 375 Antisense
GACCCGAATTGTCGCCAAGTACTGC 126 383 390 Antisense
TGTACGAGCTCCGCGAGAAGACCTC 334 559 419 Antisense
AGAAGACCTCAATCACCGTGAGCGC 87 73 434 Antisense
GTCAGTTGGATCGATGGTGGCCGGA 110 465 510 Antisense
ATCTCTGAATGCCTACGGTATCCTC 710 31 618 Antisense
GCCTGCGTGCGATCATGCACGCCAC 701 281 692 Antisense
CACGCCACTTATCGGGATTCTGGAT 24 201 709 Antisense
GCTGTGGTACGAGTTTGCCGATGAG 276 295 807 Antisense
Sequence Source Affymetrix Proprietary Database