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[193032_s_at] Yeast hypothetical 44.9 KD protein like

Description C25A1.5 /REP_DB=WormBase Gene ID /WP=CE08370 /TR=Q9XVS0 /GB=CAB02759.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Yeast hypothetical 44.9 KD protein like
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 39      4grp_high_8_8 > 20      6grp_highest_nonorm > 86      6grp_total > 59      4grp_total > 20      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 351
1 2 3 4 range
A 974.3
(-1.6)
P 		917.2
(-3)
P 		978.8
(-1.5)
P 		1156.1
(3)
P 		239
B 1004.9
(3)
P 		963.8
(1.8)
P 		804.7
(-3)
P 		971.2
(2)
P 		200
C 917.5
(-3)
P 		1019.2
(3)
P 		951.5
(-1)
P 		977.9
(0.6)
P 		102
avr 965.6
(-0.4) 		966.7
(-0.3) 		911.7
(-3) 		1035.1
(3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 561
1 2 3 4 5 6 range
A 1099.7
(3)
P 		772.4
(-2.9)
P 		765.8
(-3)
P 		864.3
(-1.2)
P 		1037.7
(1.9)
P 		1076.1
(2.6)
P 		334
B 869.8
(-1.2)
P 		784.5
(-2.1)
P 		699.5
(-3)
P 		1025.9
(0.5)
P 		1004.7
(0.3)
P 		1260.9
(3)
P 		561
C 819.4
(-1.9)
P 		886.2
(-0.9)
P 		744.4
(-3)
P 		1023.9
(1.1)
P 		837.1
(-1.6)
P 		1152.5
(3)
P 		408
avr 929.6
(-0.3) 		814.4
(-1.9) 		736.6
(-3) 		971.4
(0.3) 		959.8
(0.1) 		1163.2
(3) 		427
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 354.7
(3)
P 		199.1
(-2)
P 		255.9
(-0.2)
P 		169.2
(-3)
P 		186
B 320.4
(3)
P 		238
(-0.7)
P 		186.5
(-3)
P 		207
(-2.1)
P 		134
C 303.7
(2)
P 		293
(1.6)
P 		331.6
(3)
P 		164.9
(-3)
P 		167
avr 326.3
(3) 		243.4
(-0.4) 		258
(0.2) 		180.4
(-3) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 303
1 2 3 4 5 6 range
A 202.7
(-1.5)
P 		274.7
(1.4)
P 		166.8
(-3)
P 		219.2
(-0.9)
P 		313.1
(3)
P 		262
(0.9)
P 		146
B 302.7
(-0.3)
P 		261.1
(-1.2)
P 		169.8
(-3)
P 		271.7
(-1)
P 		408.2
(1.8)
P 		470.2
(3)
P 		300
C 303.7
(0.5)
P 		270.4
(-0.5)
P 		189.5
(-3)
P 		205.9
(-2.5)
P 		270.9
(-0.5)
P 		385.8
(3)
P 		196
avr 269.7
(-0.1) 		268.7
(-0.2) 		175.4
(-3) 		232.3
(-1.3) 		330.7
(1.7) 		372.7
(3) 		197
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193032_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1542
Sequence Type Exemplar sequence
Representative Public ID C25A1.5 NCBI
Target Description C25A1.5 /REP_DB=WormBase Gene ID /WP=CE08370 /TR=Q9XVS0 /GB=CAB02759.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Yeast hypothetical 44.9 KD protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060277(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060277 NCBI Caenorhabditis elegans C25A1.5 (C25A1.5) mRNA, complete cds. 11/11 None
GENEFINDER00000000671 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10185310:10186736:1 11/11 None
C25A1.5.2 ENSEMBL cdna:known chromosome:CEL140:I:10185296:10187040:1 gene:C25A1.5 11/11 None
C25A1.5.1 ENSEMBL cdna:known chromosome:CEL140:I:10185302:10187040:1 gene:C25A1.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000000655 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10185240-10186626(+) 100.0 100.0
Public Domain and Genome References
Gene Title Yeast hypothetical 44.9 KD protein like
Ensembl C25A1.5
Entrez Gene 172882 Entrez gene
SwissProt Q9XVS0 EMBL-EBI
WormBase CE08370 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15601_S_AT fatty acid hydroxylase (FAH1) at
ATH1-121501:267318_AT fatty acid hydroxylase (FAH1) at
CANINE_2:CFAAFFX.30776.1.S1_S_AT similar to fatty acid 2-hydroxylase cfa
CHICKEN:GGA.8879.1.S1_AT similar to LOC398669 protein gga
CHICKEN:GGAAFFX.25618.1.S1_AT similar to LOC398669 protein gga
HG-U133_PLUS_2:234963_S_AT fatty acid 2-hydroxylase hs
HG-U133B:234963_S_AT fatty acid 2-hydroxylase hs
HG-U133A:219429_AT fatty acid 2-hydroxylase hs
HG-U133_PLUS_2:219429_AT fatty acid 2-hydroxylase hs
HG-FOCUS:219429_AT fatty acid 2-hydroxylase hs
HG-U133A_2:219429_AT fatty acid 2-hydroxylase hs
HU35KSUBA:RC_AA454978_AT fatty acid 2-hydroxylase hs
U133_X3P:G13236537_3P_AT fatty acid 2-hydroxylase hs
U133_X3P:HS.249163.1.S1_3P_A_AT fatty acid 2-hydroxylase hs
HG-U95C:50926_S_AT fatty acid 2-hydroxylase hs
MG-U74BV2:112838_AT fatty acid 2-hydroxylase mm
MOE430A:1426960_A_AT fatty acid 2-hydroxylase mm
MOUSE430A_2:1426960_A_AT fatty acid 2-hydroxylase mm
MOUSE430_2:1426960_A_AT fatty acid 2-hydroxylase mm
MOUSE430_2:1458424_AT Fatty acid 2-hydroxylase, mRNA (cDNA clone IMAGE:4983638) mm
MOE430B:1458424_AT Fatty acid 2-hydroxylase, mRNA (cDNA clone IMAGE:4983638) mm
YEAST_2:1775846_AT Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Sc
Protein Similarities
Method ID Description E-Value
blast CAB02759 Hypothetical protein C25A1.5 [Caenorhabditis elegans] ref|NP_492678.1| C25A1.5 [Caenorhabditis elegans] 0.0
blast CAE66855 Hypothetical protein CBG12227 [Caenorhabditis briggsae] 0.0
blast CAB02759 Hypothetical protein C25A1.5 [Caenorhabditis elegans] ref|NP_492678.1| C25A1.5 [Caenorhabditis elegans] 1.0E-180
blast CAE66855 Hypothetical protein CBG12227 [Caenorhabditis briggsae] 1.0E-169
blast AAH04263 Fatty acid 2-hydroxylase [Homo sapiens] gb|AAH17049.2| Fatty acid 2-hydroxylase [Homo sapiens] gb|AAH02679.2| Fatty acid 2-hydroxylase [Homo sapiens] 1.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR006694 EMBL-EBI Fatty acid hydroxylase 1.2E-87
Pfam IPR001199 EMBL-EBI Cytochrome b5 4.8E-16
Pfam IPR006694 EMBL-EBI Fatty acid hydroxylase 6.9E-102
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492678.1 4 129-151,158-177,219-238,245-267
C25A1.5.2 4 129-151,158-177,219-238,245-267
C25A1.5.1 4 129-151,158-177,219-238,245-267
GENEFINDER00000000671 4 129-151,158-177,219-238,245-267
Sequence
Target Sequence 
>C. ELEGANS:193032_S_AT
ggatgccgatcgtcattactttttctatcctctcagtgctctcattttcaacatccaccg
atgtttataactcgattctcctgtggtctgcctggttcgtcattggagttttgacatgga
ctctcactgaatacagtcttcatcgatgggttttccattggaagccgtctccagattcgc
cgaatcagattcttcttcatttcttggctcatggacttcaccataaaacaccgatggacg
gagaccgccttgtgttccctccggttccagctactttgattgttggaattttctatttaa
tctactccaataccttccaatggccagtgttttgcgcgtttggcgctggaaagttatttg
gatatgtaacctacgatatggtacactattatttacatcacggaagtcctcgtccacgca
gcaatttacattttagaaaagtatatcatcataatcaccacttcaaaaactttgacgtcg
gcttcggaatttcgacttctcttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGCCGATCGTCATTACTTTTTC 330 513 419 Antisense
TCAGTGCTCTCATTTTCAACATCCA 685 623 451 Antisense
TTTATAACTCGATTCTCCTGTGGTC 506 673 482 Antisense
TCTGCCTGGTTCGTCATTGGAGTTT 3 609 505 Antisense
CATTGGAAGCCGTCTCCAGATTCGC 271 215 574 Antisense
CCGGTTCCAGCTACTTTGATTGTTG 521 265 679 Antisense
TTTAATCTACTCCAATACCTTCCAA 563 673 714 Antisense
CAATGGCCAGTGTTTTGCGCGTTTG 320 183 736 Antisense
TTTACATCACGGAAGTCCTCGTCCA 656 671 810 Antisense
CCTCGTCCACGCAGCAATTTACATT 594 267 826 Antisense
GGCTTCGGAATTTCGACTTCTCTTT 93 537 898 Antisense
Sequence Source Affymetrix Proprietary Database