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[193026_at]

Description F14E5.3 /REP_DB=WormBase Gene ID /WP=CE27964 /TR=Q19460 /GB=CAA91406.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Histidine acid phosphatases (2 domains)
Cluster 6grp_total > 80      4grp_total > 14      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 1.9
(-3)
A 		2.8
(3)
A 		2.5
(1)
A 		2.7
(2.3)
A 		1
B 1.7
(-3)
A 		3.1
(3)
A 		3.1
(3)
A 		2.9
(2.1)
A 		1
C 19.4
(1.4)
A 		4.8
(-2.3)
A 		1.9
(-3)
A 		25.5
(3)
A 		24
avr 7.7
(0.9) 		3.6
(-2.2) 		2.5
(-3) 		10.4
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 4.6
(3)
A 		1.2
(-3)
A 		1.6
(-2.3)
A 		2.6
(-0.5)
A 		1.8
(-1.9)
A 		1.2
(-3)
A 		3
B 3.1
(-1.3)
A 		1.5
(-2.7)
A 		1.5
(-2.7)
A 		8.1
(3)
A 		1.1
(-3)
A 		3.5
(-0.9)
A 		7
C 2.3
(-2.9)
A 		6.9
(-2.3)
A 		2.2
(-2.9)
A 		47.9
(3)
A 		18.8
(-0.7)
A 		1.3
(-3)
A 		47
avr 3.3
(-2.5) 		3.2
(-2.5) 		1.8
(-3) 		19.5
(3) 		7.2
(-1.2) 		2
(-2.9) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 0.7
(3)
A 		0.6
(1)
A 		0.6
(1)
A 		0.4
(-3)
A 		0
B 0.6
(-3)
A 		0.8
(3)
A 		0.7
(-0)
A 		0.6
(-3)
A 		0
C 6.4
(3)
A 		1.4
(-2.3)
A 		0.7
(-3)
A 		4.3
(0.8)
A 		6
avr 2.6
(3) 		0.9
(-2.4) 		0.7
(-3) 		1.8
(0.5) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 0.8
(3)
A 		0.4
(-1.8)
A 		0.3
(-3)
A 		0.7
(1.8)
A 		0.5
(-0.6)
A 		0.3
(-3)
A 		1
B 1.1
(-0.7)
A 		0.5
(-2.7)
A 		0.4
(-3)
A 		2.2
(3)
A 		0.4
(-3)
A 		1.3
(0)
A 		2
C 0.9
(-2.7)
A 		2.1
(-1.9)
A 		0.6
(-2.9)
A 		9.6
(3)
A 		6.1
(0.7)
A 		0.4
(-3)
A 		9
avr 0.9
(-2.2) 		1
(-2.1) 		0.4
(-3) 		4.2
(3) 		2.3
(-0) 		0.7
(-2.5) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193026_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3938
Sequence Type Exemplar sequence
Representative Public ID F14E5.3 NCBI
Target Description F14E5.3 /REP_DB=WormBase Gene ID /WP=CE27964 /TR=Q19460 /GB=CAA91406.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Histidine acid phosphatases (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063375
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000024807 ENSEMBL cdna:SNAP chromosome:CEL140:II:8432659:8437009:-1 9/11 None
GENEFINDER00000024812 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8432659:8437057:-1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_063374 7/11 Cross Hyb Matching Probes None
F14E5.6 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8432934-8437054(-) 90.84 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91406 Hypothetical protein F14E5.3 [Caenorhabditis elegans] ref|NP_495776.1| F14E5.3 [Caenorhabditis elegans] 0.0
blast CAC70090 Hypothetical protein F14E5.6 [Caenorhabditis elegans] ref|NP_495775.1| F14E5.6 [Caenorhabditis elegans] 0.0
blast CAC70090 Hypothetical protein F14E5.6 [Caenorhabditis elegans] ref|NP_495775.1| F14E5.6 [Caenorhabditis elegans] 1.0E-136
blast CAE57681 Hypothetical protein CBG00675 [Caenorhabditis briggsae] 5.0E-60
blast CAE57094 Hypothetical protein CBG24994 [Caenorhabditis briggsae] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 1.5E-10
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 1.8E-5
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 1.5E-10
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 2.0E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000024807 1 21-43
GENEFINDER00000024812 3 37-59,424-446,485-507
Sequence
Target Sequence 
>C. ELEGANS:193026_AT
atttgcaattattttcagcgaggatacaatcactcatttatgttaggaagatttctcaag
aaacgatatgtcggcacgggatttttgagtgattttatgaaacctagtgagaaaatcagt
atcgatgattctgacaagtacatcaatgactataagaacaatgttcccgtgccggatttc
atttgtgaaaacatcgaagtcatatctgaagaatatatgaaatatcgaaatttaaaaaac
ggcattgggaaaccagtaattattaagtttcggttcggcgtgcttattgatacactgttg
gagaatttaaaaactgcttggacaaacaatcttgcgaataaagaaagtttaaaattcaaa
caagattgggtgctgggaggtgttttagatgctttcggtgtatcatttacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTGCAATTATTTTCAGCGAGGAT 52 19 1495 Antisense
GCGAGGATACAATCACTCATTTATG 243 291 1512 Antisense
AACGATATGTCGGCACGGGATTTTT 388 145 1556 Antisense
GATTCTGACAAGTACATCAATGACT 168 433 1621 Antisense
GAACAATGTTCCCGTGCCGGATTTC 33 353 1650 Antisense
TCCCGTGCCGGATTTCATTTGTGAA 594 597 1659 Antisense
GAAAACATCGAAGTCATATCTGAAG 458 355 1681 Antisense
TATTAAGTTTCGGTTCGGCGTGCTT 418 663 1755 Antisense
GGTTCGGCGTGCTTATTGATACACT 601 507 1766 Antisense
GGGTGCTGGGAGGTGTTTTAGATGC 614 497 1862 Antisense
GATGCTTTCGGTGTATCATTTACTA 126 409 1882 Antisense
Sequence Source Affymetrix Proprietary Database