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[193011_at]

Description F12B6.2 /REP_DB=WormBase Gene ID /WP=CE09357 /TR=O01791 /GB=AAK21370.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=sodium dependent-phosphate transporter
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 67      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 92      6grp_total > 57      4grp_total > 03      6grp_highest_sub > Cu      6grp_high1_sub > Da      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 553
1 2 3 4 range
A 407.3
(-3)
P 		412.4
(-2.9)
P 		417.2
(-2.8)
P 		768
(3)
P 		361
B 414.9
(-2.3)
P 		419.5
(-2.3)
P 		350.5
(-3)
P 		903.6
(3)
P 		553
C 423.1
(-2)
P 		354.6
(-3)
P 		360.2
(-2.9)
P 		757.7
(3)
P 		403
avr 415.1
(-2.5) 		395.5
(-2.7) 		376
(-3) 		809.8
(3) 		434

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 365
1 2 3 4 5 6 range
A 285.7
(0)
P 		333.5
(1.4)
P 		174.6
(-3)
P 		258.4
(-0.7)
P 		214.5
(-1.9)
P 		393.2
(3)
P 		219
B 346.5
(1)
P 		281.4
(-1.1)
P 		227.8
(-2.9)
P 		223.2
(-3)
P 		242.5
(-2.4)
P 		410.2
(3)
P 		187
C 272.8
(-2.3)
P 		234.7
(-3)
P 		296.7
(-1.8)
P 		298.3
(-1.7)
P 		249.3
(-2.7)
P 		539.6
(3)
P 		305
avr 301.7
(-1.1) 		283.2
(-1.6) 		233
(-3) 		260
(-2.2) 		235.4
(-2.9) 		447.7
(3) 		215
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 148.3
(3)
P 		89.5
(-3)
P 		109.1
(-1)
P 		112.4
(-0.7)
P 		59
B 132.3
(-0.2)
P 		103.6
(-1.8)
P 		81.2
(-3)
P 		192.6
(3)
P 		111
C 140.1
(3)
P 		101.9
(-3)
P 		125.5
(0.7)
P 		127.8
(1.1)
P 		38
avr 140.2
(2.5) 		98.3
(-3) 		105.3
(-2.1) 		144.3
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 52.7
(-1.9)
P 		118.6
(3)
P 		38
(-3)
P 		65.5
(-1)
P 		64.7
(-1)
P 		95.7
(1.3)
P 		81
B 120.6
(1)
P 		93.7
(-0.6)
P 		55.3
(-3)
P 		59.1
(-2.8)
P 		98.5
(-0.3)
P 		153
(3)
P 		98
C 101.1
(-1)
P 		71.6
(-2.4)
P 		75.5
(-2.2)
P 		60
(-3)
P 		80.7
(-2)
P 		180.6
(3)
P 		121
avr 91.5
(-0.6) 		94.6
(-0.4) 		56.3
(-3) 		61.5
(-2.6) 		81.3
(-1.3) 		143.1
(3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193011_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.171
Sequence Type Exemplar sequence
Representative Public ID F12B6.2 NCBI
Target Description F12B6.2 /REP_DB=WormBase Gene ID /WP=CE09357 /TR=O01791 /GB=AAK21370.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=sodium dependent-phosphate transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058546(10), NM_171833(10), NM_058547(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058546 NCBI Caenorhabditis elegans F12B6.2a (F12B6.2) mRNA, complete cds. 10/11 None
NM_171833 NCBI Caenorhabditis elegans F12B6.2c (F12B6.2) mRNA, complete cds. 10/11 None
NM_058547 NCBI Caenorhabditis elegans F12B6.2b (F12B6.2) mRNA, complete cds. 10/11 None
SNAP00000032175 ENSEMBL cdna:SNAP chromosome:CEL140:I:2242231:2266195:1 9/11 None
GENEFINDER00000032177 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2250146:2254800:1 10/11 None
F12B6.2c ENSEMBL cdna:known chromosome:CEL140:I:2250132:2254800:1 gene:F12B6.2 10/11 None
F12B6.2b ENSEMBL cdna:known chromosome:CEL140:I:2250132:2254640:1 gene:F12B6.2 10/11 None
F12B6.2a ENSEMBL cdna:known chromosome:CEL140:I:2250132:2254893:1 gene:F12B6.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2250145-2254258(+) 95.27 100.0
Public Domain and Genome References
Ensembl F12B6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM15567 Hypothetical protein F12B6.2a [Caenorhabditis elegans] ref|NP_490947.2| F12B6.2a [Caenorhabditis elegans] 0.0
blast AAM15568 Hypothetical protein F12B6.2c [Caenorhabditis elegans] ref|NP_740804.1| F12B6.2c [Caenorhabditis elegans] 0.0
blast AAL77178 Hypothetical protein F12B6.2b [Caenorhabditis elegans] ref|NP_490948.2| F12B6.2b [Caenorhabditis elegans] 0.0
blast CAE60300 Hypothetical protein CBG03885 [Caenorhabditis briggsae] 0.0
blast AAK21371 Hypothetical protein F12B6.3 [Caenorhabditis elegans] ref|NP_490946.2| F12B6.3 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 4.9E-11
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 3.0E-9
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 8.7E-20
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490947.2 12 30-52,75-97,104-126,136-158,165-187,197-219,260-282,302-324,336-358,368-390,397-419,434-453
NP_740804.1 11 20-42,62-84,94-116,123-145,155-177,218-240,260-282,294-316,326-348,355-377,392-411
NP_490948.2 11 33-55,62-84,94-116,123-145,155-177,218-240,260-282,294-316,326-348,355-377,392-411
F12B6.2c 11 20-42,62-84,94-116,123-145,155-177,218-240,260-282,294-316,326-348,355-377,392-411
F12B6.2b 11 33-55,62-84,94-116,123-145,155-177,218-240,260-282,294-316,326-348,355-377,392-411
F12B6.2a 12 30-52,75-97,104-126,136-158,165-187,197-219,260-282,302-324,336-358,368-390,397-419,434-453
SNAP00000032175 9 195-217,224-243,258-280,439-461,471-493,534-556,576-598,618-635,645-667
GENEFINDER00000032177 13 31-53,74-91,106-125,132-154,164-186,193-215,225-247,288-310,330-352,364-386,396-418,425-447,462-481
Sequence
Target Sequence 
>C. ELEGANS:193011_AT
tgctcaaagtttcgtccttgtggctctggtgacctatcttccaaagtataatcaaatcgc
gttcaagatgaatttgacgcataacggaatttggtcttctcttccatttttcatccaaat
gatcaccaaattgttgttcgcgattattgcagacaaggttaagcagcgaaaagtgaatgc
aaccgcagttacgaaggtctcaaatgctattgccagttttgccagcgcaatcttcattgt
gatcgccgcctacggcccgtttgacagtgccgaactcgtccagctgagcataattgtatc
gatggcggcgttctcggcatatgttcccggatacaacacatcgattgttacggtggctcc
gcagttcaccgcattcatttcgtcgtatgctcagctgtacgcacaaattgcatcaacact
tgcaccgatcgttatcggacggattacgagtcatggcacaatttacgagtggaagtgtgc
attttatagtttggccggagttttggcagtgacggggctcattttccaaattttcgggca
tggtacggaaggcggcacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCAAAGTTTCGTCCTTGTGGCT 626 577 888 Antisense
AATGCTATTGCCAGTTTTGCCAGCG 29 167 1090 Antisense
GCGCAATCTTCATTGTGATCGCCGC 97 289 1112 Antisense
CCTACGGCCCGTTTGACAGTGCCGA 9 223 1136 Antisense
GTTCTCGGCATATGTTCCCGGATAC 620 441 1197 Antisense
TCGATTGTTACGGTGGCTCCGCAGT 538 603 1228 Antisense
TCACCGCATTCATTTCGTCGTATGC 512 625 1253 Antisense
CGTCGTATGCTCAGCTGTACGCACA 541 245 1268 Antisense
TCAACACTTGCACCGATCGTTATCG 593 629 1300 Antisense
TGGCAGTGACGGGGCTCATTTTCCA 516 545 1391 Antisense
CGGGCATGGTACGGAAGGCGGCACG 64 243 1422 Antisense
Sequence Source Affymetrix Proprietary Database