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[193002_at] serine/threonine kinase

Description F35H8.7 /REP_DB=WormBase Gene ID /WP=CE09940 /GEN=wee-1.1 /TR=SW:Q20085 /GB=CAA85329.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 08      4grp_total > 25      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 20.2
(-2.3)
A 		13.1
(-3)
A 		33.4
(-1)
A 		73
(3)
A 		60
B 7.5
(-3)
A 		42
(3)
A 		27.8
(0.5)
A 		21.1
(-0.6)
A 		35
C 23.4
(-3)
A 		57.2
(3)
A 		37.9
(-0.4)
A 		41.6
(0.2)
A 		34
avr 17
(-3) 		37.4
(1.3) 		33
(0.4) 		45.2
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 42.1
(-3)
A 		45.3
(-2.3)
A 		61.6
(1.3)
A 		42.2
(-3)
A 		69.4
(3)
A 		69
(2.9)
A 		27
B 32.7
(1.2)
A 		16.5
(-3)
A 		35
(1.8)
A 		39.7
(3)
A 		21.3
(-1.8)
A 		26.2
(-0.5)
A 		23
C 100.8
(3)
A 		49.8
(-0.3)
A 		26.1
(-1.9)
A 		29.8
(-1.6)
A 		8.9
(-3)
A 		29.6
(-1.6)
A 		92
avr 58.5
(3) 		37.2
(-2.1) 		40.9
(-1.2) 		37.2
(-2.1) 		33.2
(-3) 		41.6
(-1) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 7.4
(0.5)
A 		2.8
(-3)
A 		8.7
(1.5)
A 		10.7
(3)
A 		8
B 2.4
(-3)
A 		10.4
(3)
A 		6.4
(0)
A 		4.5
(-1.4)
A 		8
C 7.8
(-2.5)
A 		16.4
(3)
A 		13.2
(1)
A 		7
(-3)
A 		9
avr 5.9
(-3) 		9.9
(3) 		9.4
(2.3) 		7.4
(-0.8) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 7.8
(-3)
A 		16.1
(0.8)
A 		13.4
(-0.4)
A 		10.7
(-1.7)
A 		20.9
(3)
A 		16.8
(1.1)
A 		13
B 11.4
(3)
A 		5.5
(-3)
A 		8.5
(0.1)
A 		10.5
(2.1)
A 		8.7
(0.3)
A 		9.8
(1.4)
A 		6
C 37.3
(3)
A 		15.2
(-0.9)
A 		6.6
(-2.4)
A 		6
(-2.5)
A 		2.9
(-3)
A 		9.9
(-1.8)
A 		34
avr 18.8
(3) 		12.3
(-1) 		9.5
(-2.8) 		9.1
(-3) 		10.8
(-1.9) 		12.2
(-1.1) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193002_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3071
Sequence Type Exemplar sequence
Representative Public ID F35H8.7 NCBI
Target Description F35H8.7 /REP_DB=WormBase Gene ID /WP=CE09940 /GEN=wee-1.1 /TR=SW:Q20085 /GB=CAA85329.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063657(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063657 NCBI Caenorhabditis elegans WEE homolog family member (wee-1.1) (wee-1.1) mRNA, complete cds. 11/11 None
SNAP00000035512 ENSEMBL cdna:SNAP chromosome:CEL140:II:9581875:9583458:-1 11/11 None
F35H8.7 ENSEMBL cdna:known chromosome:CEL140:II:9581875:9583458:-1 gene:F35H8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9581871-9583455(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Gene Symbol wee-1.1
Ensembl F35H8.7
Entrez Gene 192085 Entrez gene
SwissProt Q20085 EMBL-EBI
WormBase CE09940 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85329 Hypothetical protein F35H8.7 [Caenorhabditis elegans] ref|NP_496058.1| WEE homolog family member (wee-1.1) [Caenorhabditis elegans] sp|Q20085|YPS7_CAEEL Putative serine/threonine-protein kinase F35H8.7 0.0
blast CAE57990 Hypothetical protein CBG01053 [Caenorhabditis briggsae] 2.0E-64
blast CAB16484 Hypothetical protein Y53C12A.1 [Caenorhabditis elegans] ref|NP_496095.1| WEE homolog family member (wee-1.3) [Caenorhabditis elegans] sp|O18209|PMYT1_CAEEL Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase (Myt1 kinase) (Lethal protein 37) 3.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-29
Pfam IPR000719 EMBL-EBI Protein kinase 6.6E-5
Sequence
Target Sequence 
>C. ELEGANS:193002_AT
ggaaccaacattcatctaccgagttatgggacaggatgggaaccaattagaaaatgggaa
attccagaagaaatattggaaccaatgtcagatgaacttcgagaattggtgaaacaaatg
atggacaaagccccgacacgaagaccaacttgctctgaattaatgaagaatcatgtagtg
aaagagaagctaagaataaggaaacaagaagttgaacatattccttcggagtcgcaattt
caaccgatatcctatctcacaacagaaagtccattaggatcacgggaatcatcttgctca
tctatcgattttcttgaccattttgaaaatgctgaaaaaaagacaccatttgtgagtaag
ctgaatttcgatctagaagatgatgaagacgagtatgaagtcttctctccaccaagaact
cctgtcaaaaaatcgcgataccaacaaacaatgccggaagtcagtccacccagaaagatt
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACCAACATTCATCTACCGAGTT 252 531 901 Antisense
AAATGATGGACAAAGCCCCGACACG 554 119 1016 Antisense
GAACATATTCCTTCGGAGTCGCAAT 525 351 1114 Antisense
GGAGTCGCAATTTCAACCGATATCC 300 517 1128 Antisense
CAACCGATATCCTATCTCACAACAG 324 187 1141 Antisense
TCACGGGAATCATCTTGCTCATCTA 73 627 1180 Antisense
GCTCATCTATCGATTTTCTTGACCA 425 301 1196 Antisense
GAGTATGAAGTCTTCTCTCCACCAA 224 399 1291 Antisense
CTCCACCAAGAACTCCTGTCAAAAA 233 235 1307 Antisense
AAACAATGCCGGAAGTCAGTCCACC 323 129 1346 Antisense
GAAGTCAGTCCACCCAGAAAGATTC 590 335 1357 Antisense
Sequence Source Affymetrix Proprietary Database