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[192979_at]

Description E02H4.3 /REP_DB=WormBase Gene ID /WP=CE05549 /TR=Q17917 /GB=CAA91979.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (CLK subfamily)
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 18      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 35      6grp_highest_sub > De      6grp_cgc > 13      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 381.8
(-1)
P 		466.4
(3)
P 		371.3
(-1.5)
P 		340.8
(-3)
P 		126
B 343.7
(-1.2)
P 		352.8
(-0.7)
P 		312
(-3)
P 		417.8
(3)
P 		106
C 368.6
(0.6)
P 		377.4
(1.1)
P 		308.6
(-3)
P 		409.8
(3)
P 		101
avr 364.7
(-0) 		398.9
(3) 		330.6
(-3) 		389.5
(2.2) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 346
1 2 3 4 5 6 range
A 457.1
(3)
P 		427.9
(2.3)
P 		218.6
(-3)
P 		241.9
(-2.4)
P 		312.4
(-0.6)
P 		271.5
(-1.7)
P 		239
B 556.9
(3)
P 		456
(0.6)
P 		315.2
(-2.8)
P 		379.6
(-1.3)
P 		307
(-3)
P 		361.7
(-1.7)
P 		250
C 565
(3)
P 		423.6
(0.3)
P 		278.7
(-2.5)
P 		252
(-3)
P 		332.2
(-1.5)
P 		279.8
(-2.5)
P 		313
avr 526.3
(3) 		435.8
(0.9) 		270.8
(-3) 		291.2
(-2.5) 		317.2
(-1.9) 		304.3
(-2.2) 		256
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 139
(3)
P 		101.2
(0.5)
P 		97.1
(0.2)
P 		49.9
(-3)
P 		89
B 109.6
(3)
P 		87.1
(-0.6)
P 		72.3
(-3)
P 		89
(-0.3)
P 		37
C 122
(3)
P 		108.5
(1.5)
P 		107.5
(1.4)
P 		69.1
(-3)
P 		53
avr 123.5
(3) 		98.9
(0.3) 		92.3
(-0.5) 		69.3
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 84.3
(-0.9)
P 		152.2
(3)
P 		47.6
(-3)
P 		61.4
(-2.2)
P 		94.3
(-0.3)
P 		66.1
(-1.9)
P 		105
B 193.8
(3)
P 		151.8
(0.9)
P 		76.5
(-3)
P 		100.6
(-1.8)
P 		124.7
(-0.5)
P 		134.9
(-0)
P 		117
C 209.4
(3)
P 		129.3
(-0)
P 		70.9
(-2.2)
P 		50.7
(-3)
P 		107.5
(-0.9)
P 		93.7
(-1.4)
P 		159
avr 162.5
(3) 		144.4
(1.9) 		65
(-3) 		70.9
(-2.6) 		108.8
(-0.3) 		98.2
(-1) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192979_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17826
Sequence Type Exemplar sequence
Representative Public ID E02H4.3 NCBI
Target Description E02H4.3 /REP_DB=WormBase Gene ID /WP=CE05549 /TR=Q17917 /GB=CAA91979.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (CLK subfamily)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171799(9), NM_171798(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171799 NCBI Caenorhabditis elegans E02H4.3a (E02H4.3) mRNA, complete cds. 9/11 None
NM_171798 NCBI Caenorhabditis elegans E02H4.3b (E02H4.3) mRNA, complete cds. 9/11 None
E02H4.3a ENSEMBL cdna:known chromosome:CEL140:X:14284755:14291229:-1 gene:E02H4.3 9/11 A 
E02H4.3b ENSEMBL cdna:known chromosome:CEL140:X:14285395:14293912:-1 gene:E02H4.3 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000018169 5/11 Cross Hyb Matching Probes None
SNAP00000018156 5/11 Cross Hyb Matching Probes None
SNAP00000016454 4/11 Cross Hyb Matching Probes None
GENEFINDER00000016459 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14285394-14290859(-) 100.0 100.0
Public Domain and Genome References
Ensembl E02H4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94122 Hypothetical protein E02H4.3a [Caenorhabditis elegans] emb|CAA91979.2| Hypothetical protein E02H4.3a [Caenorhabditis elegans] ref|NP_741928.1| E02H4.3a [Caenorhabditis elegans] 0.0
blast CAE63011 Hypothetical protein CBG07253 [Caenorhabditis briggsae] 0.0
blast CAD44096 Hypothetical protein E02H4.3b [Caenorhabditis elegans] emb|CAD44105.1| Hypothetical protein E02H4.3b [Caenorhabditis elegans] ref|NP_741927.1| E02H4.3b [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-54
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 2.0E-55
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 3.0E-49
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 5.8E-59
Pfam IPR000719 EMBL-EBI Protein kinase 5.8E-59
Sequence
Target Sequence 
>C. ELEGANS:192979_AT
tggaaagtttgtgcttcgccgattcaaaattgatgattgttttcagcctttgcgagttct
tcacagtacacacgtgaggttaattgatttcggatcggcaaccttcgatcatgagcatca
ttcaattattgtctcgacacgacattaccgagctccggaagtcattcttgaacttggctg
gtctcaaccgtgtgacgtttggtccatcggatgtatcctatacgagctctacactggcgt
cactctcttccaaactcacgaaaaccgcgaacacttggcaatgatggaacgcgtgctcgg
tgacattccgctcagaatggcaaagaggacaaaaaccaaattcttcatcaatggtcggct
tgattgggtgaacacttcagctgacgcagcctacgtgagagacaattgcaaaccattaag
acgttccatgtcttgtacagacccagaacacgtggagctgtttgaactcattgagaacat
gttgatgtttgagcctcttgctcgtatgaaacttcccgaagcacttcagcatcgctactt
taatcggttgccgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAAAGTTTGTGCTTCGCCGATTC 415 545 2109 Antisense
GATTGTTTTCAGCCTTTGCGAGTTC 470 433 2143 Antisense
TGCGAGTTCTTCACAGTACACACGT 149 585 2159 Antisense
GGCAACCTTCGATCATGAGCATCAT 180 535 2205 Antisense
GTCATTCTTGAACTTGGCTGGTCTC 440 467 2269 Antisense
GTCTCAACCGTGTGACGTTTGGTCC 264 467 2289 Antisense
TATCCTATACGAGCTCTACACTGGC 640 657 2322 Antisense
AGCTGACGCAGCCTACGTGAGAGAC 43 81 2487 Antisense
AGCCTCTTGCTCGTATGAAACTTCC 566 83 2600 Antisense
GAAACTTCCCGAAGCACTTCAGCAT 64 357 2616 Antisense
ATCGCTACTTTAATCGGTTGCCGGA 676 33 2639 Antisense
Sequence Source Affymetrix Proprietary Database