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[192978_at] MULTIPLE EXOSTOSES HOMOLOG 2

Description K01G5.6 /REP_DB=WormBase Gene ID /WP=CE16196 /GEN=rib-2 /TR=O17920 /GB=CAB07245.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=MULTIPLE EXOSTOSES HOMOLOG 2
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 28      4grp_high_8_8 > 0      6grp_highest_nonorm > 61      6grp_total > 26      4grp_total > 07      6grp_highest_sub > Cu      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 452.5
(-3)
P 		492.2
(0.8)
P 		514.8
(3)
P 		507.2
(2.3)
P 		62
B 541.1
(3)
P 		335.9
(-3)
P 		471.4
(1)
P 		461.2
(0.7)
P 		205
C 395.4
(-3)
P 		453.1
(2.1)
P 		463.2
(3)
P 		435.3
(0.5)
P 		68
avr 463
(0.8) 		427.1
(-3) 		483.1
(3) 		467.9
(1.4) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 172
1 2 3 4 5 6 range
A 478.8
(3)
P 		434.1
(1.4)
P 		306.8
(-3)
P 		381.5
(-0.4)
P 		343.9
(-1.7)
P 		389.7
(-0.1)
P 		172
B 461.2
(3)
P 		455
(2.7)
P 		441.9
(1.9)
P 		387.9
(-1.1)
P 		354.5
(-3)
P 		394.8
(-0.7)
P 		107
C 470.6
(3)
P 		465.6
(2.8)
P 		414.7
(0.5)
P 		415.7
(0.5)
P 		338.7
(-3)
P 		405.3
(0)
P 		132
avr 470.2
(3) 		451.6
(2.1) 		387.8
(-1) 		395
(-0.6) 		345.7
(-3) 		396.6
(-0.5) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 164.7
(3)
P 		106.8
(-0.8)
P 		134.6
(1)
P 		74.2
(-3)
P 		91
B 172.5
(3)
P 		82.9
(-3)
P 		109.2
(-1.2)
P 		98.3
(-2)
P 		90
C 130.9
(0.9)
P 		130.3
(0.9)
P 		161.4
(3)
P 		73.4
(-3)
P 		88
avr 156
(3) 		106.7
(-1) 		135.1
(1.3) 		82
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 88.3
(-1.5)
P 		154.4
(3)
P 		66.8
(-3)
P 		96.8
(-0.9)
P 		103.8
(-0.5)
P 		94.9
(-1.1)
P 		88
B 160.5
(3)
P 		151.5
(2.1)
P 		107.3
(-2.5)
P 		102.7
(-3)
P 		144
(1.3)
P 		147.2
(1.6)
P 		58
C 174.4
(3)
P 		142.1
(0.9)
P 		105.6
(-1.5)
P 		83.6
(-3)
P 		109.6
(-1.3)
P 		135.7
(0.4)
P 		91
avr 141.1
(2.1) 		149.3
(3) 		93.2
(-3) 		94.4
(-2.9) 		119.1
(-0.2) 		125.9
(0.5) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192978_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7769
Sequence Type Exemplar sequence
Representative Public ID K01G5.6 NCBI
Target Description K01G5.6 /REP_DB=WormBase Gene ID /WP=CE16196 /GEN=rib-2 /TR=O17920 /GB=CAB07245.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=MULTIPLE EXOSTOSES HOMOLOG 2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066967(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066967 NCBI Caenorhabditis elegans related to mammalian RIB-affecting EXT gene family member (rib-2) (rib-2) mRNA, complete cds. 11/11 None
GENEFINDER00000033501 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10742476:10746402:1 11/11 None
SNAP00000033487 ENSEMBL cdna:SNAP chromosome:CEL140:III:10742751:10746402:1 11/11 None
K01G5.6 ENSEMBL cdna:known chromosome:CEL140:III:10742687:10746533:1 gene:K01G5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10742746-10746398(+) 100.0 100.0
Public Domain and Genome References
Gene Title MULTIPLE EXOSTOSES HOMOLOG 2
Gene Symbol rib-2
Ensembl K01G5.6
Entrez Gene 176502 Entrez gene
SwissProt O01705 EMBL-EBI
WormBase CE16196 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.13145.1.S1_AT similar to exostoses (multiple)-like 3 cfa
DROSOPHILA_2:1636990_AT brother of tout-velu dm
DROSGENOME1:153992_AT brother of tout-velu dm
CHICKEN:GGAAFFX.10650.2.S1_S_AT similar to exostoses (multiple)-like 3 gga
CHICKEN:GGAAFFX.10650.3.S1_S_AT similar to exostoses (multiple)-like 3 gga
HG-U133_PLUS_2:209202_S_AT exostoses (multiple)-like 3 hs
HG-U133A_2:209202_S_AT exostoses (multiple)-like 3 hs
HG-U133A:209202_S_AT exostoses (multiple)-like 3 hs
HG-FOCUS:209202_S_AT exostoses (multiple)-like 3 hs
HG-U133_PLUS_2:211051_S_AT exostoses (multiple)-like 3 hs
HG-U133A:211051_S_AT exostoses (multiple)-like 3 hs
HG-U133A_2:211051_S_AT exostoses (multiple)-like 3 hs
HUGENEFL:U96629_RNA2_AT exostoses (multiple)-like 3 hs
U133_X3P:G2897904_3P_A_AT exostoses (multiple)-like 3 hs
U133_X3P:G13623512_3P_S_AT exostoses (multiple)-like 3 hs
HG-U95AV2:38809_S_AT exostoses (multiple)-like 3 hs
MOUSE430_2:1421339_AT exostoses (multiple)-like 3 mm
MOE430A:1421339_AT exostoses (multiple)-like 3 mm
MOUSE430A_2:1421339_AT exostoses (multiple)-like 3 mm
MU11KSUBA:AA030551_S_AT exostoses (multiple)-like 3 mm
MG-U74AV2:95591_AT exostoses (multiple)-like 3 mm
MOE430A:1434703_AT exostoses (multiple)-like 3 mm
MOUSE430A_2:1434703_AT exostoses (multiple)-like 3 mm
MOUSE430_2:1434703_AT exostoses (multiple)-like 3 mm
MU19KSUBA:TC19950_AT Exostoses (multiple)-like 3 (Extl3), mRNA mm
RAE230A:1387489_AT exostoses (multiple)-like 3 rn
RAT230_2:1387489_AT exostoses (multiple)-like 3 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07245 Hypothetical protein K01G5.6 [Caenorhabditis elegans] ref|NP_499368.1| related to mammalian RIB-affecting EXT gene family member (rib-2) [Caenorhabditis elegans] sp|O01705|EXT2_CAEEL Exostosin-2 (Glucuronyl-galactosyl-proteoglycan/Glucuronosyl-N- acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase) (Multiple exostoses homolog 2) dbj|BAB83878.1| heparan sulfate GlcNAc transferase-I/II [Caenorhabditis elegans] 0.0
blast AAC47510 multiple exostoses homolog 2 [Caenorhabditis elegans] 0.0
blast CAE71408 Hypothetical protein CBG18318 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004263 EMBL-EBI Exostosin-like 7.3E-9
Pfam IPR004263 EMBL-EBI Exostosin-like 7.3E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499368.1 1 13-35
K01G5.6 1 13-35
GENEFINDER00000033501 1 52-74
SNAP00000033487 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:192978_AT
tcgttgcacattcctgtcgagttcattcgtgtcgccgagaacaatttgaacaatcgattc
gtgccgtgggatcggatcgagacggaagcggtgctatcgctggacgatgatatcgatctg
atgcagcaggagatcatactcgcattccgagtttggcgggagaatcgagatcgaattgtt
ggattcccggcacgtcatcacgccagatacggcgattcgatgttctataacagcaatcat
acgtgtcagatgagtatgattctcactggtgcagcgtttattcataagaactatttgact
gcttacacctacgaaatgccagctgaaattcgtgaacatgtgaatagcatcaaaaactgc
gaggacattgctatgaattatttagtttctcatctcacacgaaaaccgccaatcaaaacg
acttctcgatggacgctcaaatgtccaacttgcacggaaagcttgtataaagaagggaca
catttcgagaaacgccacgaatgtatgcgattgttcacgaaaatctacggctacaatcct
ctgaaattctcccaattccgtgctgactcgattcttttcaagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTTGCACATTCCTGTCGAGTTCA 116 603 1831 Antisense
CGAGTTCATTCGTGTCGCCGAGAAC 543 247 1848 Antisense
GATTCGTGCCGTGGGATCGGATCGA 160 433 1886 Antisense
GCAGGAGATCATACTCGCATTCCGA 495 311 1956 Antisense
GCACGTCATCACGCCAGATACGGCG 586 315 2020 Antisense
GTATGATTCTCACTGGTGCAGCGTT 460 455 2084 Antisense
GACTGCTTACACCTACGAAATGCCA 225 375 2127 Antisense
TTATTTAGTTTCTCATCTCACACGA 590 679 2208 Antisense
GACGCTCAAATGTCCAACTTGCACG 242 381 2262 Antisense
AAATCTACGGCTACAATCCTCTGAA 121 119 2351 Antisense
GTGCTGACTCGATTCTTTTCAAGAC 276 479 2390 Antisense
Sequence Source Affymetrix Proprietary Database