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[192975_at] ubiquitin-activating enzyme

Description F11H8.1 /REP_DB=WormBase Gene ID /WP=CE04374 /GEN=rfl-1 /TR=Q19360 /GB=AAA81160.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ubiquitin-activating enzyme
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 96      6grp_total > 85      4grp_total > 41      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 554.5
(-2.6)
P 		549.5
(-3)
P 		563.8
(-1.8)
P 		621.5
(3)
P 		72
B 482.6
(-3)
P 		612.8
(3)
P 		612
(3)
P 		559.6
(0.5)
P 		130
C 618.8
(1)
P 		619.4
(1)
P 		652.2
(3)
P 		553.3
(-3)
P 		99
avr 552
(-3) 		593.9
(1.4) 		609.3
(3) 		578.1
(-0.3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 686
1 2 3 4 5 6 range
A 597.7
(-2.4)
P 		549.7
(-3)
P 		694.3
(-1.1)
P 		759.3
(-0.3)
P 		1016.5
(3)
P 		837.2
(0.7)
P 		467
B 562.1
(-3)
P 		561.7
(-3)
P 		738.5
(-1)
P 		840.1
(0.1)
P 		1049.2
(2.5)
P 		1095.2
(3)
P 		534
C 442.7
(-3)
P 		577.8
(-1.8)
P 		668
(-1)
P 		968.6
(1.6)
P 		694.9
(-0.8)
P 		1128.4
(3)
P 		686
avr 534.2
(-3) 		563.1
(-2.6) 		700.3
(-0.9) 		856
(1) 		920.2
(1.8) 		1020.3
(3) 		486
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 201.9
(3)
P 		119.3
(-1.5)
P 		147.4
(0.1)
P 		91
(-3)
P 		111
B 153.9
(3)
P 		151.3
(2.5)
P 		141.8
(0.9)
P 		119.3
(-3)
P 		35
C 204.8
(2)
P 		178.1
(0.8)
P 		227.3
(3)
P 		93.3
(-3)
P 		134
avr 186.9
(3) 		149.6
(0.4) 		172.2
(2) 		101.2
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 316
1 2 3 4 5 6 range
A 110.2
(-3)
P 		195.5
(-0.4)
P 		151.2
(-1.7)
P 		192.6
(-0.5)
P 		306.7
(3)
P 		203.8
(-0.1)
P 		197
B 195.6
(-2.6)
P 		187
(-2.8)
P 		179.3
(-3)
P 		222.5
(-2)
P 		426.3
(3)
P 		408.4
(2.6)
P 		247
C 164.1
(-3)
P 		176.3
(-2.7)
P 		170
(-2.8)
P 		194.8
(-2.1)
P 		224.9
(-1.3)
P 		377.7
(3)
P 		214
avr 156.6
(-3) 		186.3
(-2) 		166.8
(-2.6) 		203.3
(-1.4) 		319.3
(2.6) 		330
(3) 		173
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192975_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6517
Sequence Type Exemplar sequence
Representative Public ID F11H8.1 NCBI
Target Description F11H8.1 /REP_DB=WormBase Gene ID /WP=CE04374 /GEN=rfl-1 /TR=Q19360 /GB=AAA81160.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ubiquitin-activating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066133(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066133 NCBI Caenorhabditis elegans ectopic membrane RuFfLes in embryo family member (rfl-1) (rfl-1) mRNA, complete cds. 11/11 None
SNAP00000017878 ENSEMBL cdna:SNAP chromosome:CEL140:III:7021819:7023495:1 11/11 None
GENEFINDER00000017883 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7021819:7023495:1 11/11 None
F11H8.1 ENSEMBL cdna:known chromosome:CEL140:III:7021814:7023512:1 gene:F11H8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7021815-7023492(+) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin-activating enzyme
Gene Symbol rfl-1
Ensembl F11H8.1
Entrez Gene 175982 Entrez gene
SwissProt Q19360 EMBL-EBI
WormBase CE34538 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249926_AT ubiquitin activating enzyme, putative (ECR1) at
CANINE:1604229_AT similar to ubiquitin-activating enzyme E1C isoform 1 cfa
CANINE:1604006_AT similar to ubiquitin-activating enzyme E1C isoform 1 cfa
CANINE_2:CFA.1092.1.S1_AT similar to ubiquitin-activating enzyme E1C isoform 1 cfa
CANINE_2:CFAAFFX.10771.1.S1_S_AT similar to ubiquitin-activating enzyme E1C isoform 1 cfa
DROSOPHILA_2:1639153_AT dm
DROSGENOME1:141247_AT dm
CHICKEN:GGA.4137.1.S1_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) gga
HU35KSUBA:RC_AA620323_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HU35KSUBD:RC_AA401968_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U133A:209115_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U133_PLUS_2:209115_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U133A_2:209115_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
U133_X3P:G5912116_3P_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U95AV2:40066_AT ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
U133_X3P:1556339_3P_A_AT Ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U133_PLUS_2:1556338_AT Ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U133_PLUS_2:1556339_A_AT Ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
HG-U95E:72708_AT Ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
U133_X3P:HS2.231711.1.S1_3P_S_AT Ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) hs
MG-U74AV2:92818_AT ubiquitin-activating enzyme E1C mm
MOE430A:1431746_A_AT ubiquitin-activating enzyme E1C mm
MOUSE430A_2:1431746_A_AT ubiquitin-activating enzyme E1C mm
MOUSE430_2:1431746_A_AT ubiquitin-activating enzyme E1C mm
MOE430A:1435164_S_AT ubiquitin-activating enzyme E1C mm
MOUSE430A_2:1435164_S_AT ubiquitin-activating enzyme E1C mm
MOUSE430_2:1435164_S_AT ubiquitin-activating enzyme E1C mm
MOE430A:1417202_S_AT ubiquitin-activating enzyme E1C mm
MOUSE430A_2:1417202_S_AT ubiquitin-activating enzyme E1C mm
MOUSE430_2:1417202_S_AT ubiquitin-activating enzyme E1C mm
MG-U74BV2:108309_AT Ubiquitin-activating enzyme E1C, mRNA (cDNA clone MGC:5766 IMAGE:3590282) mm
MG-U74CV2:137915_AT Ubiquitin-activating enzyme E1C, mRNA (cDNA clone MGC:5766 IMAGE:3590282) mm
MG-U74BV2:165326_AT Ubiquitin-activating enzyme E1C, mRNA (cDNA clone MGC:5766 IMAGE:3590282) mm
MU19KSUBB:TC28496_AT Ubiquitin-activating enzyme E1C, mRNA (cDNA clone MGC:5766 IMAGE:3590282) mm
MU19KSUBB:TC28496_G_AT Ubiquitin-activating enzyme E1C, mRNA (cDNA clone MGC:5766 IMAGE:3590282) mm
MU19KSUBC:TC37513_AT Ubiquitin-activating enzyme E1C, mRNA (cDNA clone MGC:5766 IMAGE:3590282) mm
RG-U34C:RC_AI105086_AT ubiquitin-activating enzyme E1C rn
RAE230A:1398813_AT ubiquitin-activating enzyme E1C rn
RAT230_2:1398813_AT ubiquitin-activating enzyme E1C rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4839 ubiquitin activating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81160 Ectopic membrane ruffles in embryo protein 1 [Caenorhabditis elegans] ref|NP_498534.2| ectopic membrane RuFfLes in embryo family member (rfl-1) [Caenorhabditis elegans] 0.0
blast CAE70067 Hypothetical protein CBG16503 [Caenorhabditis briggsae] 0.0
blast EAA09425 ENSANGP00000009925 [Anopheles gambiae str. PEST] ref|XP_313978.1| ENSANGP00000009925 [Anopheles gambiae str. PEST] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR000127 EMBL-EBI Ubiquitin-activating enzyme repeat 1.5E-34
Pfam IPR000594 EMBL-EBI UBA/THIF-type NAD/FAD binding fold 5.2E-59
Sequence
Target Sequence 
>C. ELEGANS:192975_AT
ttgaaggtgtttctcttgacgctgacgatccgatccacgttgaatgggttctcgaaagag
caagtcttcgtgcagaaaaatacaatattcgaggtgtggatcgtcgtttgacatctggag
ttctaaaaagaattattccggctgttgcatcaacaaatgcagttatcgctgcatcatgtg
ccctagaagctctgaaattggccacaaacattgccaaaccaatcgataattatcttaatt
tcactcaaattcacggagcatataccagtgttgtttcaatgatgaaagatgacaattgtc
tcacttgtagtggtgggcgtcttccattcgaagtttcaccatcttcaactcttgaatcgc
ttatcatcagactctcggagcgtttccatctcaaacatccgacactagcgacctcaactc
gaaaactttactgtattagcagtttcatgccacaatttgaacaagaaagcaaagaaaatc
tgcatacttcgatgaaagatcttgttagcgatggcgaagaaatactggtatccgacgaag
cattgtctcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAAGGTGTTTCTCTTGACGCTGA 136 703 746 Antisense
GACGATCCGATCCACGTTGAATGGG 45 379 769 Antisense
GGTGTGGATCGTCGTTTGACATCTG 330 499 838 Antisense
GAATTATTCCGGCTGTTGCATCAAC 273 325 875 Antisense
GCTGCATCATGTGCCCTAGAAGCTC 502 297 913 Antisense
ACTTGTAGTGGTGGGCGTCTTCCAT 254 101 1048 Antisense
TCGAAGTTTCACCATCTTCAACTCT 488 601 1073 Antisense
TCAACTCTTGAATCGCTTATCATCA 60 631 1090 Antisense
TCATCAGACTCTCGGAGCGTTTCCA 230 621 1109 Antisense
CTCAAACATCCGACACTAGCGACCT 662 227 1135 Antisense
GGTATCCGACGAAGCATTGTCTCGT 558 505 1272 Antisense
Sequence Source Affymetrix Proprietary Database