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[192970_at]

Description T14E8.3 /REP_DB=WormBase Gene ID /WP=CE04958 /TR=Q22490 /GB=AAA82386.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor (family 1)
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 92      6grp_highest_nonorm > 91      6grp_total > 84      4grp_total > 49      6grp_highest_sub > 05      6grp_high1_sub > 04_05      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 81.8
(-3)
A 		322.2
(2.8)
P 		329.4
(3)
P 		146.2
(-1.4)
A 		248
B 53.3
(-3)
A 		308.5
(3)
P 		303.1
(2.9)
P 		97.6
(-2)
A 		255
C 68
(-3)
A 		314.2
(3)
P 		295.9
(2.6)
P 		129
(-1.5)
A 		246
avr 67.7
(-3) 		315
(3) 		309.5
(2.9) 		124.3
(-1.6) 		247

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 141.4
(-3)
P 		214.2
(1.6)
P 		155.1
(-2.1)
P 		212.6
(1.5)
P 		236.9
(3)
P 		228.9
(2.5)
P 		96
B 112.5
(-3)
P 		186.5
(-0.2)
P 		135.5
(-2.1)
P 		205.6
(0.5)
P 		271
(3)
P 		207.2
(0.6)
P 		159
C 96.1
(-3)
P 		188.2
(0.8)
P 		160.1
(-0.3)
P 		240
(3)
P 		237.7
(2.9)
P 		212.7
(1.9)
P 		144
avr 116.7
(-3) 		196.3
(0.6) 		150.2
(-1.5) 		219.4
(1.7) 		248.5
(3) 		216.3
(1.5) 		132
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 29.8
(-2.2)
A 		69.9
(1.5)
P 		86.1
(3)
P 		21.4
(-3)
A 		65
B 17
(-3)
A 		76.2
(3)
P 		70.2
(2.4)
P 		20.8
(-2.6)
A 		59
C 22.5
(-2.9)
A 		90.3
(2.1)
P 		103.1
(3)
P 		21.7
(-3)
A 		81
avr 23.1
(-2.8) 		78.8
(2.3) 		86.5
(3) 		21.3
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 26.1
(-3)
P 		76.2
(3)
P 		33.8
(-2.1)
P 		53.9
(0.3)
P 		71.5
(2.4)
P 		55.7
(0.5)
P 		50
B 39.2
(-2.5)
P 		62.1
(-0.7)
P 		32.9
(-3)
P 		54.5
(-1.3)
P 		110.1
(3)
P 		77.3
(0.5)
P 		77
C 35.6
(-3)
P 		57.4
(0.2)
P 		40.7
(-2.3)
P 		48.3
(-1.2)
P 		76.9
(3)
P 		71.2
(2.2)
P 		41
avr 33.6
(-3) 		65.2
(0.6) 		35.8
(-2.7) 		52.2
(-0.9) 		86.2
(3) 		68.1
(0.9) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192970_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17107
Sequence Type Exemplar sequence
Representative Public ID T14E8.3 NCBI
Target Description T14E8.3 /REP_DB=WormBase Gene ID /WP=CE04958 /TR=Q22490 /GB=AAA82386.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor (family 1)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029738(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029738 NCBI Caenorhabditis elegans T14E8.4 (T14E8.4) mRNA, complete cds. 11/11 None
SNAP00000007185 ENSEMBL cdna:SNAP chromosome:CEL140:X:6559525:6561329:-1 11/11 None
GENEFINDER00000007189 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6559525:6563946:-1 11/11 None
T14E8.4 ENSEMBL cdna:known chromosome:CEL140:X:6560141:6562200:-1 gene:T14E8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6560073-6569473(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T14E8.4
Entrez Gene 3565421 Entrez gene
SwissProt Q5WRS0 EMBL-EBI
WormBase CE37676 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV28332 Hypothetical protein T14E8.4 [Caenorhabditis elegans] ref|NP_001024909.1| T14E8.4 [Caenorhabditis elegans] 0.0
blast T16876 hypothetical protein T14E8.3 - Caenorhabditis elegans 0.0
blast CAE65753 Hypothetical protein CBG10840 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:192970_AT
gtgcacaagtttttgccaaacccgtataccacaaaaaacccaaatacattgaaaaacgat
atcatcagaacgaccagaacaattattcccgtcgttgacaaacatacgtacgttccatgg
aactataaagtagacgctgttcaggtgtctactttaacaagtgctttagcaccagttaaa
cctacggaaacagtaatatcctcccctccagttgcatttaatttcaagttacctgctgag
cagacttctacacaaccattcagagttgaaataaattggcatgatgacgaagttaagcag
gaaccaacaaaggcaccaggagttttcgtctccccatggtatctaaagacaccatcacat
ataccaccggaaattgaattcgctacaccaacgccattgatcagttcgccactagaagct
gtgagcccaattctcagccaattgaaatcaaactcgttgaatttcacggaactcggaaac
caggcaaacaactacttctctgcagcatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCACAAGTTTTTGCCAAACCCGT 276 475 2677 Antisense
GACCAGAACAATTATTCCCGTCGTT 289 383 2748 Antisense
GACGCTGTTCAGGTGTCTACTTTAA 486 379 2809 Antisense
TCCCCTCCAGTTGCATTTAATTTCA 653 595 2878 Antisense
TGCTGAGCAGACTTCTACACAACCA 357 583 2910 Antisense
ACAAAGGCACCAGGAGTTTTCGTCT 497 111 2983 Antisense
CACCATCACATATACCACCGGAAAT 535 201 3026 Antisense
GCTACACCAACGCCATTGATCAGTT 701 301 3058 Antisense
TGATCAGTTCGCCACTAGAAGCTGT 157 565 3074 Antisense
GAAGCTGTGAGCCCAATTCTCAGCC 203 343 3091 Antisense
AAACAACTACTTCTCTGCAGCATTA 680 127 3162 Antisense
Sequence Source Affymetrix Proprietary Database