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[192961_at] ubiquitin carboxyl-terminal hydrolase

Description T27A3.2 /REP_DB=WormBase Gene ID /WP=CE14219 /TR=P91502 /GB=AAB42297.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=ubiquitin carboxyl-terminal hydrolase
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 66      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 82      6grp_total > 66      4grp_total > 56      6grp_highest_sub > Da      6grp_high1_sub > Da      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 282
1 2 3 4 range
A 290.6
(-3)
P 		429.8
(0.8)
P 		510.3
(3)
P 		328.5
(-2)
P 		220
B 296.2
(-3)
P 		423.8
(0.6)
P 		506.4
(3)
P 		295.6
(-3)
P 		211
C 334.7
(-1.5)
P 		440.8
(0.8)
P 		544.3
(3)
P 		262.2
(-3)
P 		282
avr 307.2
(-2.7) 		431.5
(0.6) 		520.3
(3) 		295.4
(-3) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 225
1 2 3 4 5 6 range
A 373.3
(-1.1)
P 		406.7
(-0.1)
P 		349.7
(-1.7)
P 		413
(0.1)
P 		304.5
(-3)
P 		516.6
(3)
P 		212
B 339.6
(-1.5)
P 		357.3
(-0.6)
P 		368.3
(-0)
P 		396.5
(1.4)
P 		309.1
(-3)
P 		428.4
(3)
P 		119
C 434.9
(1.7)
P 		291.7
(-3)
P 		472.9
(2.9)
P 		319.8
(-2.1)
P 		476.4
(3)
P 		465.3
(2.6)
P 		185
avr 382.6
(-1.4) 		351.9
(-3) 		397
(-0.7) 		376.4
(-1.8) 		363.3
(-2.4) 		470.1
(3) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 146
1 2 3 4 range
A 105.8
(1.1)
P 		93.3
(0.2)
P 		133.4
(3)
P 		48.1
(-3)
P 		85
B 94.5
(0.5)
P 		104.7
(1.6)
P 		117.3
(3)
P 		63
(-3)
P 		54
C 110.8
(-0.3)
P 		126.7
(0.4)
P 		189.7
(3)
P 		44.2
(-3)
P 		146
avr 103.7
(0.3) 		108.2
(0.6) 		146.8
(3) 		51.8
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 68.8
(-3)
P 		144.7
(3)
P 		76.2
(-2.4)
P 		104.7
(-0.2)
P 		91.9
(-1.2)
P 		125.8
(1.5)
P 		76
B 118.2
(-0.5)
P 		118.9
(-0.5)
P 		89.4
(-3)
P 		105
(-1.7)
P 		125.6
(0.1)
P 		159.7
(3)
P 		70
C 161.2
(3)
P 		89
(-1.5)
P 		120.4
(0.5)
P 		64.3
(-3)
P 		154.2
(2.6)
P 		155.8
(2.7)
P 		97
avr 116.1
(-0.3) 		117.5
(-0.2) 		95.3
(-2.6) 		91.3
(-3) 		123.9
(0.5) 		147.1
(3) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192961_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.827
Sequence Type Exemplar sequence
Representative Public ID T27A3.2 NCBI
Target Description T27A3.2 /REP_DB=WormBase Gene ID /WP=CE14219 /TR=P91502 /GB=AAB42297.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=ubiquitin carboxyl-terminal hydrolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059364(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059364 NCBI Caenorhabditis elegans T27A3.2 (T27A3.2) mRNA, complete cds. 11/11 None
SNAP00000022771 ENSEMBL cdna:SNAP chromosome:CEL140:I:6118274:6122457:1 11/11 None
GENEFINDER00000022784 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6118274:6122457:1 11/11 None
T27A3.2 ENSEMBL cdna:known chromosome:CEL140:I:6118269:6122672:1 gene:T27A3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6118273-6122457(+) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin carboxyl-terminal hydrolase
Ensembl T27A3.2
Entrez Gene 172296 Entrez gene
SwissProt P91502 EMBL-EBI
WormBase CE30696 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42297 Hypothetical protein T27A3.2 [Caenorhabditis elegans] ref|NP_491765.2| T27A3.2 [Caenorhabditis elegans] 0.0
blast CAE72952 Hypothetical protein CBG20283 [Caenorhabditis briggsae] 0.0
blast XP_867393 PREDICTED: similar to Ubiquitin isopeptidase T isoform 2 [Canis familiaris] 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR001607 EMBL-EBI Zn-finger in ubiquitin thiolesterase 1.6E-32
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.0E-8
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.7E-9
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 4.8E-123
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.0E-8
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.7E-9
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.9E-116
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.0E-8
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.7E-9
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 2.8E-124
Sequence
Target Sequence 
>C. ELEGANS:192961_AT
acaactccagatattccagcaagtgttcgttttgttgctggagagcttatgttgatggga
ttctgtgagaatgcgtgctacagagcagcttattattccaatggaaatgtggagattgca
tcaaactggctcatggagcatatggacgactctgatatcaatgatttgtttgtgattcca
tctggaactccatcagctcgtggagaagtcgatccgaatcttgtcgcgtcgattgttgaa
atgggattctctaatcatcaagcaaagtatgctttgaagcaggtgccgactgttgcagag
gctgttgaatggctttttgccaatatggatagtatcccagtagagtcagccgccgtcggt
ctaagctcggatgctccagagccgtctgtcacagaatcggcaactcaaaagacattcaag
gatggtggagagaaatataagttgattgggatgatttctcatatgggatcacgtcctgat
tctggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAACTCCAGATATTCCAGCAAGTG 350 113 1681 Antisense
AAGTGTTCGTTTTGTTGCTGGAGAG 482 159 1701 Antisense
GAATGCGTGCTACAGAGCAGCTTAT 316 329 1749 Antisense
TCTGGAACTCCATCAGCTCGTGGAG 588 605 1861 Antisense
AAGTCGATCCGAATCTTGTCGCGTC 261 157 1886 Antisense
GAAGCAGGTGCCGACTGTTGCAGAG 434 341 1956 Antisense
GAGGCTGTTGAATGGCTTTTTGCCA 128 405 1978 Antisense
ATAGTATCCCAGTAGAGTCAGCCGC 40 23 2009 Antisense
TCGGTCTAAGCTCGGATGCTCCAGA 81 605 2036 Antisense
GTCTGTCACAGAATCGGCAACTCAA 405 471 2064 Antisense
TATGGGATCACGTCCTGATTCTGGT 550 655 2142 Antisense
Sequence Source Affymetrix Proprietary Database