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[192946_s_at] protein disulfide isomerase

Description C14B1.1 /REP_DB=WormBase Gene ID /WP=CE00897 /GEN=pdi-1 /TR=Q17967 /GB=CAA85491.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=protein disulfide isomerase
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 08      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 04      6grp_total > 19      4grp_total > 51      6grp_cgc > 01      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4808
1 2 3 4 range
A 5027.8
(0.7)
P 		6229.4
(3)
P 		6156.1
(2.9)
P 		3028.4
(-3)
P 		3201
B 3986.9
(-2.3)
P 		7835.9
(3)
P 		6070.7
(0.6)
P 		3497.3
(-3)
P 		4339
C 5191.5
(-0.2)
P 		7480.7
(3)
P 		5881
(0.8)
P 		3137.2
(-3)
P 		4344
avr 4735.4
(-0.7) 		7182
(3) 		6035.9
(1.3) 		3221
(-3) 		3961

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3208
1 2 3 4 5 6 range
A 6334.8
(3)
P 		5275.6
(0.3)
P 		3973.5
(-3)
P 		5119.3
(-0.1)
P 		4345
(-2.1)
P 		3987.5
(-3)
P 		2361
B 6572.3
(3)
P 		4725.1
(-0.5)
P 		4341.4
(-1.2)
P 		4740
(-0.4)
P 		4593.2
(-0.7)
P 		3364
(-3)
P 		3208
C 6253.1
(3)
P 		4262.9
(-1.7)
P 		5422.1
(1)
P 		4882
(-0.3)
P 		6010.2
(2.4)
P 		3726.8
(-3)
P 		2526
avr 6386.7
(3) 		4754.5
(-0.6) 		4579
(-1) 		4913.8
(-0.3) 		4982.8
(-0.1) 		3692.8
(-3) 		2694
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1707
1 2 3 4 range
A 1830.6
(3)
P 		1352.2
(0.9)
P 		1609.3
(2)
P 		443.3
(-3)
P 		1387
B 1271.4
(-0.3)
P 		1935.3
(3)
P 		1406.7
(0.3)
P 		745.3
(-3)
P 		1190
C 1718.5
(1.4)
P 		2150.7
(3)
P 		2049.7
(2.6)
P 		529
(-3)
P 		1622
avr 1606.8
(2) 		1812.7
(3) 		1688.6
(2.4) 		572.5
(-3) 		1240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1453
1 2 3 4 5 6 range
A 1167.8
(-1.2)
P 		1876.3
(3)
P 		865.3
(-3)
P 		1298.4
(-0.4)
P 		1311.1
(-0.4)
P 		970.8
(-2.4)
P 		1011
B 2286.8
(3)
P 		1572.8
(-0.5)
P 		1054.2
(-3)
P 		1255.5
(-2)
P 		1866.2
(1)
P 		1254.4
(-2)
P 		1233
C 2317.8
(3)
P 		1300.7
(-1.6)
P 		1380
(-1.2)
P 		981.8
(-3)
P 		1944.8
(1.3)
P 		1247.6
(-1.8)
P 		1336
avr 1924.1
(3) 		1583.3
(0.5) 		1099.8
(-3) 		1178.6
(-2.4) 		1707.4
(1.4) 		1157.6
(-2.6) 		824
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192946_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7797
Sequence Type Exemplar sequence
Representative Public ID C14B1.1 NCBI
Target Description C14B1.1 /REP_DB=WormBase Gene ID /WP=CE00897 /GEN=pdi-1 /TR=Q17967 /GB=CAA85491.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=protein disulfide isomerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065345(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065345 NCBI Caenorhabditis elegans Protein Disulfide Isomerase family member (pdi-1) (pdi-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000040973 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3699785:3701433:-1 11/11 None
SNAP00000040957 ENSEMBL cdna:SNAP chromosome:CEL140:III:3699830:3701433:-1 11/11 None
C14B1.1.2 ENSEMBL cdna:known chromosome:CEL140:III:3699717:3701454:-1 gene:C14B1.1 11/11 A 
C14B1.1.3 ENSEMBL cdna:known chromosome:CEL140:III:3699717:3701526:-1 gene:C14B1.1 11/11 None
C14B1.1.1 ENSEMBL cdna:known chromosome:CEL140:III:3699717:3701439:-1 gene:C14B1.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3699829-3701433(-) 99.66 100.0
Public Domain and Genome References
Gene Title protein disulfide isomerase
Gene Symbol pdi-1
Ensembl C14B1.1
Entrez Gene 175472 Entrez gene
SwissProt Q17967 EMBL-EBI
WormBase CE00897 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5783 endoplasmic reticulum inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
16853 isomerase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85491 Hypothetical protein C14B1.1 [Caenorhabditis elegans] ref|NP_497746.1| Protein Disulfide Isomerase family member (pdi-1) [Caenorhabditis elegans] sp|Q17967|PDI1_CAEEL Protein disulfide-isomerase 1 precursor (PDI 1) (Prolyl 4-hydroxylase beta 1 subunit) gb|AAB94647.1| protein disulphide isomerase isoform I [Caenorhabditis elegans] 0.0
blast CAB40204 disulphide isomerase [Caenorhabditis briggsae] emb|CAB40200.1| disulphide isomerase [Caenorhabditis briggsae] emb|CAE64479.1| Hypothetical protein CBG09203 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.0E-47
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 9.7
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.0E-40
Sequence
Target Sequence 
>C. ELEGANS:192946_S_AT
gaagttccgtgctaagatcgtctttgttcttctcgacgttgatgttgaagagaacgcaag
aattctcgagttcctcggagtcgatgccaagaacaccccagccaacagaattgtcagcct
tgccgatcaagttgagaagttcaagccacaagaaggagaagatttcgaagctttcaccaa
ctcatatttggaaggaaagtctgctcaagacttgaaggctcaagatcttccagaagattg
gaatgcactcccagttaaggttctcgtcgcctccaacttcaacgaaattgcccttgatga
aaccaagactgtcttcgtcaaattctacgccccatggtgcggacattgcaagcaactggt
tccagtttgggatgagcttgctgagaagtacgagtccaacccaaatgttgtcattgcaaa
gcttgatgctactctcaacgagcttgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTTCCGTGCTAAGATCGTCTTT 69 337 852 Antisense
AGATCGTCTTTGTTCTTCTCGACGT 51 63 866 Antisense
ACGCAAGAATTCTCGAGTTCCTCGG 495 91 905 Antisense
CTCGGAGTCGATGCCAAGAACACCC 48 233 925 Antisense
ACACCCCAGCCAACAGAATTGTCAG 518 109 944 Antisense
GAATTGTCAGCCTTGCCGATCAAGT 302 323 959 Antisense
GATTTCGAAGCTTTCACCAACTCAT 632 427 1012 Antisense
TGCACTCCCAGTTAAGGTTCTCGTC 669 577 1095 Antisense
GTCGCCTCCAACTTCAACGAAATTG 606 469 1117 Antisense
GACTGTCTTCGTCAAATTCTACGCC 631 375 1158 Antisense
TGATGCTACTCTCAACGAGCTTGCC 235 567 1275 Antisense
Sequence Source Affymetrix Proprietary Database