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[192945_at] potassium channel protein

Description ZK1067.5 /REP_DB=WormBase Gene ID /WP=CE03842 /GEN=twk-20 /TR=Q23386 /GB=CAA93881.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_total > 11      4grp_total > 12      6grp_cgc > 63      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 34.4
(-1)
A 		24.9
(-1.7)
A 		4.2
(-3)
A 		97
(3)
P 		93
B 39
(2.4)
A 		42.1
(3)
A 		8.6
(-3)
A 		35.8
(1.9)
A 		34
C 29
(-1.8)
A 		4.2
(-3)
A 		17.3
(-2.3)
A 		123.7
(3)
P 		120
avr 34.1
(-1.1) 		23.7
(-1.9) 		10
(-3) 		85.5
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 4.7
(-3)
A 		18.8
(0.7)
A 		23.7
(2)
A 		27.1
(2.9)
A 		27.5
(3)
A 		13.5
(-0.7)
A 		23
B 46.7
(3)
A 		40.5
(2.1)
A 		34.9
(1.3)
A 		3.9
(-3)
A 		30.4
(0.7)
A 		12.2
(-1.8)
A 		43
C 16.5
(-1.6)
A 		62.4
(3)
A 		25.1
(-0.8)
A 		43.4
(1.1)
A 		2.9
(-3)
A 		25.6
(-0.7)
A 		60
avr 22.6
(-1.6) 		40.6
(3) 		27.9
(-0.2) 		24.8
(-1) 		20.3
(-2.2) 		17.1
(-3) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 12.5
(2.2)
A 		5.4
(-1)
A 		1.1
(-3)
A 		14.2
(3)
P 		13
B 12.4
(3)
A 		10.4
(1.8)
A 		2
(-3)
A 		7.6
(0.2)
A 		10
C 9.6
(-0.4)
A 		1.2
(-3)
A 		6
(-1.5)
A 		20.9
(3)
P 		20
avr 11.5
(1.6) 		5.7
(-1.6) 		3
(-3) 		14.2
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 0.9
(-3)
A 		6.7
(1.7)
A 		5.2
(0.5)
A 		6.9
(1.9)
A 		8.3
(3)
A 		3.3
(-1.1)
A 		7
B 16.3
(3)
A 		13.5
(1.9)
A 		8.5
(-0.1)
A 		1
(-3)
A 		12.3
(1.4)
A 		4.5
(-1.6)
A 		15
C 6.1
(-1.3)
A 		19
(3)
A 		6.4
(-1.2)
A 		8.7
(-0.4)
A 		0.9
(-3)
A 		8.6
(-0.4)
A 		18
avr 7.8
(-1.2) 		13.1
(3) 		6.7
(-2.1) 		5.5
(-3) 		7.2
(-1.7) 		5.5
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192945_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3556
Sequence Type Exemplar sequence
Representative Public ID ZK1067.5 NCBI
Target Description ZK1067.5 /REP_DB=WormBase Gene ID /WP=CE03842 /GEN=twk-20 /TR=Q23386 /GB=CAA93881.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063560(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063560 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-4) (twk-4) mRNA, complete cds. 11/11 None
GENEFINDER00000014745 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9185040:9194206:-1 10/11 None
ZK1067.5 ENSEMBL cdna:known chromosome:CEL140:II:9190031:9194206:-1 gene:ZK1067.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9190027-9194203(-) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein
Gene Symbol twk-4
Ensembl ZK1067.5
Entrez Gene 192079 Entrez gene
SwissProt Q23386 EMBL-EBI
WormBase CE03842 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93881 Hypothetical protein ZK1067.5 [Caenorhabditis elegans] ref|NP_495961.1| TWiK family of potassium channels family member (twk-4) [Caenorhabditis elegans] 0.0
blast CAE57870 Hypothetical protein CBG00910 [Caenorhabditis briggsae] 1.0E-177
blast CAE57870 Hypothetical protein CBG00910 [Caenorhabditis briggsae] 1.0E-173
blast AAO65265 putative RNA binding protein SYM-2 [Caenorhabditis elegans] emb|CAA93887.2| Hypothetical protein ZK1067.6 [Caenorhabditis elegans] emb|CAA92704.2| Hypothetical protein ZK1067.6 [Caenorhabditis elegans] ref|NP_495960.2| ZK1067.6 [Caenorhabditis elegans] 1.0E-130
blast CAB07286 Hypothetical protein T28A8.1 [Caenorhabditis elegans] ref|NP_499790.1| TWiK family of potassium channels family member (twk-40) [Caenorhabditis elegans] 3.0E-36
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495961.1 8 7-26,99-121,130-152,222-244,251-273,293-315,320-337,352-374
ZK1067.5 8 7-26,99-121,130-152,222-244,251-273,293-315,320-337,352-374
GENEFINDER00000014745 7 7-26,87-109,179-201,211-233,253-275,280-302,309-331
Sequence
Target Sequence 
>C. ELEGANS:192945_AT
caactacagtactcacttctataggttacggtaatcttatcccaatttcaactggcggca
aaattttctgtgttggatatgcaatttttggaattccgctaactcttgtgacaattgcgg
atttagcaaaatttgtggcagatatgctaattatggatccaacggaagacccaaaaactg
gcagacagctacttgttctagtatttttacttggttatatgacaatttctgcatgtgtgt
atactattttagagccgatgtggtcatttttagactctttttatttttgtttagtttcat
tgttaacagtaggatttggtgatctacatccagttggaaccgttgagtatatgctttgtt
ccattgtttttatttttatcggtcttatacttacaactcttgctgttgacgtgagcggat
cagttggaattgcaaagatgcattcgatcggtcgaggttttgacgcaatgaagatgttga
atgcgttacgcaaaaaagagacagtcaagaagttccaaataatgttggaactcatgggtt
actcgtctaaggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACTACAGTACTCACTTCTATAGG 434 185 704 Antisense
TAGGTTACGGTAATCTTATCCCAAT 563 651 725 Antisense
TTATCCCAATTTCAACTGGCGGCAA 492 677 740 Antisense
TGGAATTCCGCTAACTCTTGTGACA 707 545 792 Antisense
ACTGGCAGACAGCTACTTGTTCTAG 407 97 880 Antisense
TGGTGATCTACATCCAGTTGGAACC 640 549 1020 Antisense
GTATATGCTTTGTTCCATTGTTTTT 577 453 1050 Antisense
TCGGTCTTATACTTACAACTCTTGC 13 605 1082 Antisense
ACTCTTGCTGTTGACGTGAGCGGAT 623 99 1099 Antisense
GATCGGTCGAGGTTTTGACGCAATG 709 417 1149 Antisense
AACTCATGGGTTACTCGTCTAAGGC 176 141 1232 Antisense
Sequence Source Affymetrix Proprietary Database