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[192944_at] glutathione S-transferase

Description F11G11.1 /REP_DB=WormBase Gene ID /WP=CE07054 /GEN=gst-8 /TR=P91254 /GB=AAB37847.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Glutathione S-transferase
Cluster 6grp_total > 17      4grp_total > 22      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 33.3
(-2.4)
A 		23
(-3)
A 		23.5
(-3)
A 		126.1
(3)
A 		103
B 103.5
(3)
A 		5.2
(-3)
A 		12.8
(-2.5)
A 		13.6
(-2.5)
A 		98
C 57.3
(3)
A 		33.5
(0.3)
A 		4.4
(-3)
A 		51.2
(2.3)
A 		53
avr 64.7
(3) 		20.6
(-2.2) 		13.6
(-3) 		63.6
(2.9) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 207
1 2 3 4 5 6 range
A 159.9
(3)
P 		84.8
(0)
A 		78.4
(-0.2)
P 		8.3
(-3)
A 		60.2
(-0.9)
A 		47.1
(-1.5)
A 		152
B 207.3
(3)
P 		84.8
(-1.1)
A 		83.8
(-1.1)
A 		44.8
(-2.4)
A 		26.5
(-3)
A 		38.5
(-2.6)
A 		181
C 214.6
(3)
P 		69.3
(-1.2)
A 		53.7
(-1.7)
A 		7.7
(-3)
A 		62
(-1.4)
A 		57.2
(-1.6)
A 		207
avr 193.9
(3) 		79.6
(-1) 		72
(-1.2) 		20.3
(-3) 		49.6
(-2) 		47.6
(-2.1) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 12.1
(0.2)
A 		5
(-3)
A 		6.1
(-2.5)
A 		18.5
(3)
A 		14
B 33
(3)
A 		1.3
(-3)
A 		3
(-2.7)
A 		2.9
(-2.7)
A 		32
C 19
(3)
A 		9.6
(-0.2)
A 		1.5
(-3)
A 		8.6
(-0.6)
A 		18
avr 21.4
(3) 		5.3
(-2.4) 		3.5
(-3) 		10
(-0.8) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 29.5
(2.9)
P 		30.1
(3)
A 		17.1
(0.2)
P 		2.1
(-3)
A 		18.2
(0.4)
A 		11.5
(-1)
A 		28
B 72.1
(3)
P 		28.2
(-1.3)
A 		20.4
(-2.1)
A 		11.9
(-2.9)
A 		10.8
(-3)
A 		14.3
(-2.7)
A 		61
C 79.5
(3)
P 		21.1
(-1.5)
A 		13.7
(-2.1)
A 		1.6
(-3)
A 		20.1
(-1.6)
A 		19.1
(-1.7)
A 		78
avr 60.4
(3) 		26.5
(-0.7) 		17.1
(-1.7) 		5.2
(-3) 		16.4
(-1.8) 		15
(-1.9) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192944_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4207
Sequence Type Exemplar sequence
Representative Public ID F11G11.1 NCBI
Target Description F11G11.1 /REP_DB=WormBase Gene ID /WP=CE07054 /GEN=gst-8 /TR=P91254 /GB=AAB37847.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Glutathione S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062483(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062483 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-8) (gst-8) mRNA, complete cds. 11/11 None
F11G11.1 ENSEMBL cdna:known chromosome:CEL140:II:4883740:4884536:1 gene:F11G11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4883737-4884534(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-8
Ensembl F11G11.1
Entrez Gene 184364 Entrez gene
SwissProt P91254 EMBL-EBI
WormBase CE07054 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494884 Glutathione S-Transferase family member (gst-8) [Caenorhabditis elegans] gb|AAB37847.1| Glutathione s-transferase protein 8 [Caenorhabditis elegans] sp|P91254|GST8_CAEEL Probable glutathione S-transferase 8 (GST class-sigma) 1.0E-117
blast NP_494883 Glutathione S-Transferase family member (gst-7) [Caenorhabditis elegans] gb|AAB37846.1| Glutathione s-transferase protein 7 [Caenorhabditis elegans] sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (GST class-sigma) 1.0E-101
blast CAE58992 Hypothetical protein CBG02265 [Caenorhabditis briggsae] emb|CAE56959.1| Hypothetical protein CBG24809 [Caenorhabditis briggsae] 2.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 1.9E-6
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.0E-19
Sequence
Target Sequence 
>C. ELEGANS:192944_AT
ctacaagctatcatatttccccatccgtggagctggagaggttattcgacagatatttgt
atatgctggacaaagctttgaggatcatagaatttcaatagaggaatgggcagctgtcaa
accaaccaccccattcgggcaacttccacttctagaggtcgatggaaaggttcttccaca
atctcattctatcgctcgctttttggctcgtcaattcggaatcaatggaaagtgtgcatg
ggaggaggctcaagtcaactcgatcgctgatcagttcaaagattatcgcaacgaagttcg
tccatatgtgatggtgaagatgggatttgctgaaggagatcttgatgctctttccaagga
tgtctttcttccaggattcaagaagcactacggattcatttataatttccttaaaactgc
aggatccggttacttggttggagactcgctgacgtttgtggatttgctcattgctcagca
cactgcggatatattgagtactgatccagctcttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACAAGCTATCATATTTCCCCATC 684 225 21 Antisense
TTCCCCATCCGTGGAGCTGGAGAGG 5 699 37 Antisense
AATGGGCAGCTGTCAAACCAACCAC 472 161 125 Antisense
GCAACTTCCACTTCTAGAGGTCGAT 520 319 159 Antisense
AAAGGTTCTTCCACAATCTCATTCT 199 121 186 Antisense
GATGCTCTTTCCAAGGATGTCTTTC 64 415 364 Antisense
GGATGTCTTTCTTCCAGGATTCAAG 697 513 378 Antisense
ACTGCAGGATCCGGTTACTTGGTTG 429 95 436 Antisense
GGAGACTCGCTGACGTTTGTGGATT 448 519 460 Antisense
ATTTGCTCATTGCTCAGCACACTGC 492 17 482 Antisense
TATTGAGTACTGATCCAGCTCTTCT 637 663 512 Antisense
Sequence Source Affymetrix Proprietary Database