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[192933_at] homology with E.coli lepA protein

Description ZK1236.1 /REP_DB=WormBase Gene ID /WP=CE01446 /TR=SW:P34617 /GB=AAA28191.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=homology with E.coli lepA protein
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 53      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 81      6grp_total > 20      4grp_total > 47      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 325
(3)
P 		243.1
(-3)
P 		258.8
(-1.8)
P 		288.2
(0.3)
P 		82
B 335.7
(3)
P 		240.6
(-1)
P 		275.4
(0.5)
P 		193.3
(-3)
P 		142
C 298.8
(2)
P 		249.6
(-3)
P 		251.7
(-2.8)
P 		308.8
(3)
P 		59
avr 319.8
(3) 		244.4
(-3) 		262
(-1.6) 		263.4
(-1.5) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 213.8
(-3)
P 		284.7
(1.6)
P 		296
(2.4)
P 		305.9
(3)
P 		305.2
(3)
P 		242.4
(-1.1)
P 		92
B 240.8
(-3)
P 		337.4
(3)
P 		331.2
(2.6)
P 		288.7
(-0)
P 		272.7
(-1)
P 		280.6
(-0.5)
P 		97
C 202.4
(-1.7)
P 		327.7
(3)
P 		313.5
(2.5)
P 		248.1
(0)
P 		168.4
(-3)
P 		310.3
(2.3)
P 		159
avr 219
(-3) 		316.6
(3) 		313.6
(2.8) 		280.9
(0.8) 		248.8
(-1.2) 		277.8
(0.6) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 118.3
(3)
P 		52.8
(-2.2)
P 		67.7
(-1)
P 		42.2
(-3)
P 		76
B 107
(3)
P 		59.4
(-1.3)
P 		63.8
(-0.9)
P 		41.2
(-3)
P 		66
C 98.9
(3)
P 		71.8
(-0.5)
P 		87.7
(1.6)
P 		52.1
(-3)
P 		47
avr 108.1
(3) 		61.3
(-1.5) 		73.1
(-0.3) 		45.2
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 39.4
(-3)
P 		101.2
(3)
P 		64.5
(-0.6)
P 		77.6
(0.7)
P 		92.1
(2.1)
P 		59
(-1.1)
P 		62
B 83.8
(-1.8)
P 		112.3
(3)
P 		80.4
(-2.3)
P 		76.5
(-3)
P 		110.8
(2.7)
P 		104.6
(1.7)
P 		36
C 75
(-0.2)
P 		100
(2.6)
P 		79.8
(0.3)
P 		49.9
(-3)
P 		54.5
(-2.5)
P 		103.9
(3)
P 		54
avr 66.1
(-3) 		104.5
(3) 		74.9
(-1.6) 		68
(-2.7) 		85.8
(0.1) 		89.2
(0.6) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192933_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7773
Sequence Type Exemplar sequence
Representative Public ID ZK1236.1 NCBI
Target Description ZK1236.1 /REP_DB=WormBase Gene ID /WP=CE01446 /TR=SW:P34617 /GB=AAA28191.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=homology with E.coli lepA protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066462(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066462 NCBI Caenorhabditis elegans ZK1236.1 (ZK1236.1) mRNA, complete cds. 11/11 None
SNAP00000024908 ENSEMBL cdna:SNAP chromosome:CEL140:III:8429254:8432108:1 11/11 None
GENEFINDER00000024916 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8429254:8431436:1 11/11 None
ZK1236.1 ENSEMBL cdna:known chromosome:CEL140:III:8429251:8431461:1 gene:ZK1236.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8429249-8431432(+) 100.0 100.0
Public Domain and Genome References
Gene Title homology with E.coli lepA protein
Ensembl ZK1236.1
Entrez Gene 176191 Entrez gene
SwissProt P34617 EMBL-EBI
WormBase CE01446 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249430_AT GTP-binding protein LepA, putative at
CANINE_2:CFAAFFX.3722.1.S1_S_AT similar to CG32527-PB, isoform B cfa
CHICKEN:GGAAFFX.11790.1.S1_AT similar to Hypothetical protein FLJ13220 gga
HG-U133_PLUS_2:222770_S_AT hypothetical protein FLJ13220 hs
HG-U133B:222770_S_AT hypothetical protein FLJ13220 hs
HG-U133_PLUS_2:218884_S_AT hypothetical protein FLJ13220 hs
HG-U133A:218884_S_AT hypothetical protein FLJ13220 hs
HG-U133A_2:218884_S_AT hypothetical protein FLJ13220 hs
HU35KSUBA:H88128_S_AT hypothetical protein FLJ13220 hs
HU35KSUBD:RC_AA227903_AT hypothetical protein FLJ13220 hs
HU35KSUBD:RC_H88129_AT hypothetical protein FLJ13220 hs
U133_X3P:G11345459_3P_A_AT hypothetical protein FLJ13220 hs
U133_X3P:HS.41127.0.S2_3P_A_AT hypothetical protein FLJ13220 hs
HG-U95B:42629_I_AT hypothetical protein FLJ13220 hs
HG-U95B:42630_R_AT hypothetical protein FLJ13220 hs
HG-U95C:60279_AT hypothetical protein FLJ13220 hs
HG-U95E:78282_AT hypothetical protein FLJ13220 hs
HG-U95E:78333_AT hypothetical protein FLJ13220 hs
HG-U95E:78973_AT hypothetical protein FLJ13220 hs
MU11KSUBA:AA671597_AT expressed sequence AA407526 mm
MG-U74AV2:160956_R_AT expressed sequence AA407526 mm
MG-U74BV2:165399_I_AT expressed sequence AA407526 mm
MOE430B:1455163_AT expressed sequence AA407526 mm
MOUSE430_2:1455163_AT expressed sequence AA407526 mm
MOE430B:1437184_AT expressed sequence AA407526 mm
MOUSE430_2:1437184_AT expressed sequence AA407526 mm
YEAST_2:1776341_AT Mitochondrial GTPase of unknown function, similar to E. coli elongation factor-type GTP-binding protein LepA and to LK1236.1 from Caenorhabditis elegans Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA28191 Hypothetical protein ZK1236.1 [Caenorhabditis elegans] ref|NP_498863.1| ZK1236.1 [Caenorhabditis elegans] sp|P34617|YO81_CAEEL Hypothetical GTP-binding protein ZK1236.1 in chromosome III 0.0
blast CAE71257 Hypothetical protein CBG18137 [Caenorhabditis briggsae] 0.0
blast S44896 ZK1236.1 protein - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 2.9E-32
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-9
Pfam IPR006297 EMBL-EBI GTP-binding protein LepA 5.8E-32
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 1.6E-59
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 2.9E-32
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-9
Pfam IPR006297 EMBL-EBI GTP-binding protein LepA 1.3E-43
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 8.3E-70
Sequence
Target Sequence 
>C. ELEGANS:192933_AT
gatgtagatagcttcctagaaccaatggtaaaagtccgaatgattgttccaaatgaaatg
atgggtactgtcaatggattgtgctccgaatgtcgtggtgaacgaggagagatcacatcg
atcgattcgactcgtctcgtgatcctttggaggcttccacttgcagaagttgctgttgat
ttctttgaaagactcaagaaattgacaagtggatatgcatcgtttgactatgagcccgat
ggatggcaggatactcggctggtgaaacttacaattcttatcaacagtaaagaggtcagt
gaattctcgcaaatcataccagcggcaatggcgagagatcgtgcaaagatcttagttcaa
cgtctgaaacgagaaattccacgacagcaatttgaagttacaattaaagcttgcattggt
tcctcaacgaaagcattatctcaaattgttatccagccaatgaaacgagacttctcgcag
ctattgaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTAGATAGCTTCCTAGAACCAA 309 409 1327 Antisense
AATGGATTGTGCTCCGAATGTCGTG 413 163 1399 Antisense
CATCGATCGATTCGACTCGTCTCGT 454 211 1442 Antisense
TCCTTTGGAGGCTTCCACTTGCAGA 469 591 1469 Antisense
ATGCATCGTTTGACTATGAGCCCGA 470 43 1541 Antisense
GAGCCCGATGGATGGCAGGATACTC 186 385 1558 Antisense
GGATACTCGGCTGGTGAAACTTACA 620 505 1575 Antisense
GTGAATTCTCGCAAATCATACCAGC 590 481 1625 Antisense
ATCATACCAGCGGCAATGGCGAGAG 146 29 1639 Antisense
TAAAGCTTGCATTGGTTCCTCAACG 294 641 1731 Antisense
ACGAGACTTCTCGCAGCTATTGAAG 219 93 1791 Antisense
Sequence Source Affymetrix Proprietary Database