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[192931_at] sodium/potasssium transporting ATPase

Description C01G12.8 /REP_DB=WormBase Gene ID /WP=CE15577 /TR=O45240 /GB=CAB07586.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=sodium-potasssium transporting ATPase
Cluster 6grp_total > 88      4grp_total > 18      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 9.8
(-3)
A 		22.1
(2.1)
A 		16.7
(-0.1)
A 		24.3
(3)
A 		15
B 25.6
(-3)
A 		40.8
(0.2)
A 		54.1
(2.9)
A 		54.4
(3)
A 		29
C 60.2
(-0.9)
A 		71
(-0.2)
A 		26.2
(-3)
A 		122.6
(3)
A 		96
avr 31.9
(-3) 		44.6
(-0.8) 		32.3
(-2.9) 		67.1
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 6.6
(-3)
A 		38
(-2.3)
A 		11.3
(-2.9)
A 		30.8
(-2.4)
A 		146.7
(0.2)
P 		267.4
(3)
P 		261
B 22.1
(-2.6)
A 		61.3
(-1.4)
A 		7.1
(-3)
A 		24.5
(-2.5)
A 		110.5
(-0)
P 		215.3
(3)
P 		208
C 38.4
(-2.4)
A 		36.5
(-2.4)
A 		11.3
(-3)
A 		9.9
(-3)
A 		155.2
(0.1)
P 		295.7
(3)
P 		286
avr 22.4
(-2.7) 		45.3
(-2.1) 		9.9
(-3) 		21.7
(-2.7) 		137.5
(0.1) 		259.5
(3) 		250
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 3.6
(-3)
A 		4.8
(3)
A 		4.4
(1)
A 		3.6
(-3)
A 		1
B 8.2
(-3)
A 		10.1
(-0.3)
A 		12.5
(3)
A 		11.6
(1.7)
A 		4
C 19.9
(2.6)
A 		20.4
(2.8)
A 		9.1
(-3)
A 		20.7
(3)
A 		12
avr 10.6
(0.5) 		11.8
(2.6) 		8.7
(-3) 		12
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 1.2
(-3)
A 		13.5
(-1.8)
A 		2.5
(-2.9)
A 		7.8
(-2.4)
A 		44.3
(1)
P 		65.1
(3)
P 		64
B 7.7
(-2.5)
A 		20.4
(-1.6)
A 		1.7
(-3)
A 		6.5
(-2.6)
A 		44.9
(0.3)
P 		80.3
(3)
P 		79
C 14.2
(-2.2)
A 		11.1
(-2.4)
A 		2.9
(-2.9)
A 		2
(-3)
A 		50.2
(-0)
P 		99
(3)
P 		97
avr 7.7
(-2.6) 		15
(-2) 		2.4
(-3) 		5.4
(-2.8) 		46.5
(0.3) 		81.5
(3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192931_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3496
Sequence Type Exemplar sequence
Representative Public ID C01G12.8 NCBI
Target Description C01G12.8 /REP_DB=WormBase Gene ID /WP=CE15577 /TR=O45240 /GB=CAB07586.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=sodium-potasssium transporting ATPase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064633(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064633 NCBI Caenorhabditis elegans C01G12.8 (C01G12.8) mRNA, complete cds. 11/11 None
SNAP00000003956 ENSEMBL cdna:SNAP chromosome:CEL140:II:14596872:14597504:1 9/11 None
GENEFINDER00000003971 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14596872:14597823:1 9/11 None
C01G12.8 ENSEMBL cdna:known chromosome:CEL140:II:14593415:14597508:1 gene:C01G12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14593411-14597501(+) 100.0 100.0
Public Domain and Genome References
Gene Title sodium/potasssium transporting ATPase
Ensembl C01G12.8
Entrez Gene 175120 Entrez gene
SwissProt O45240 EMBL-EBI
WormBase CE15577 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
15672 monovalent inorganic cation transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
15077 monovalent inorganic cation transporter activity inferred from electronic annotation QuickGO AmiGO
15662 ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism inferred from electronic annotation QuickGO AmiGO
16820 hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03818 Hypothetical protein C01G12.8 [Caenorhabditis elegans] emb|CAB07586.1| Hypothetical protein C01G12.8 [Caenorhabditis elegans] ref|NP_497034.1| C01G12.8 [Caenorhabditis elegans] 0.0
blast CAE58506 Hypothetical protein CBG01656 [Caenorhabditis briggsae] 0.0
blast XP_618149 PREDICTED: similar to Na+/K+ -ATPase alpha 4 subunit isoform 1 [Bos taurus] 0.0
blast CAB03818 Hypothetical protein C01G12.8 [Caenorhabditis elegans] emb|CAB07586.1| Hypothetical protein C01G12.8 [Caenorhabditis elegans] ref|NP_497034.1| C01G12.8 [Caenorhabditis elegans] 1.0E-111
blast CAE58506 Hypothetical protein CBG01656 [Caenorhabditis briggsae] 1.0E-102
blast CAB03818 Hypothetical protein C01G12.8 [Caenorhabditis elegans] emb|CAB07586.1| Hypothetical protein C01G12.8 [Caenorhabditis elegans] ref|NP_497034.1| C01G12.8 [Caenorhabditis elegans] 1.0E-97
blast CAE58506 Hypothetical protein CBG01656 [Caenorhabditis briggsae] 3.0E-92
Protein Families
Method ID Description E-Value
ec A1A4_HUMAN (Q13733) Sodium/potassium-transporting ATPase alpha-4 chain (EC 3.6.3.9) (Sodium pump 4) (Na+/K+ ATPase 4) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.1E-60
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 2.5E-54
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.1E-60
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 3.3E-97
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.5E-7
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.5E-9
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.5E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497034.1 8 111-133,148-167,308-330,340-362,816-838,876-898,977-999,1009-1027
C01G12.8 8 111-133,148-167,308-330,340-362,816-838,876-898,977-999,1009-1027
SNAP00000003956 4 21-43,82-101,121-143,153-170
GENEFINDER00000003971 3 21-43,122-144,151-170
Sequence
Target Sequence 
>C. ELEGANS:192931_AT
gccagccatctcatttgcatacgaggttccagaaagcgacatcatgcagagagctccgcg
taatccgctctacgacaagctcgtcaacaaacgactcgtcatgttctcctacatgcaaat
cggagcgattcaagcgtgcgccggcttcaccacctactttgtgttgatgatgtcgaacgg
atggttcccgcaggatttgttgaatattagtgagcaatgggataacaagtatatcgatga
tttggaggacagttatggacaacaatggagttatgaaagccgaaaagctctggagtcttg
ctgttatggtactttcttcttcacgattgtggtcacccagtggtctgatttgtttgcttc
gaaaactagaaagaactctttggttatgcaaggaatggaaaaccatgtgctcaatacatc
agtgatcttcacctgcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCAGCCATCTCATTTGCATACGAG 104 279 2541 Antisense
GCGACATCATGCAGAGAGCTCCGCG 401 289 2576 Antisense
ACAAACGACTCGTCATGTTCTCCTA 601 111 2627 Antisense
ATCGGAGCGATTCAAGCGTGCGCCG 339 35 2659 Antisense
GCTTCACCACCTACTTTGTGTTGAT 163 305 2684 Antisense
GAACGGATGGTTCCCGCAGGATTTG 328 347 2715 Antisense
GCTCTGGAGTCTTGCTGTTATGGTA 183 299 2827 Antisense
TGTTATGGTACTTTCTTCTTCACGA 434 561 2842 Antisense
TCACGATTGTGGTCACCCAGTGGTC 447 627 2861 Antisense
AGTGGTCTGATTTGTTTGCTTCGAA 267 59 2879 Antisense
TACATCAGTGATCTTCACCTGCTTC 218 645 2955 Antisense
Sequence Source Affymetrix Proprietary Database