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[192922_s_at] TBA-2 tubulin alpha-2 chain

Description F26E4.8 /REP_DB=WormBase Gene ID /WP=CE09692 /GEN=tba-2 /TR=O18688 /GB=CAB03001.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=TBA-2 tubulin alpha-2 chain
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 01      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 06      6grp_total > 23      4grp_total > 51      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7330
1 2 3 4 range
A 7756.1
(-1.6)
P 		12753.4
(3)
P 		9985
(0.5)
P 		6203.1
(-3)
P 		6550
B 9186.1
(1.1)
P 		10785
(3)
P 		10094.3
(2.2)
P 		5725.8
(-3)
P 		5059
C 7274.1
(-0.9)
P 		10827.8
(3)
P 		8427.6
(0.3)
P 		5423
(-3)
P 		5405
avr 8072.1
(-0.6) 		11455.4
(3) 		9502.3
(0.9) 		5784
(-3) 		5671

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5107
1 2 3 4 5 6 range
A 10906.4
(3)
P 		7712.2
(-2.4)
P 		7898
(-2.1)
P 		7964.9
(-1.9)
P 		7600.9
(-2.6)
P 		7333.9
(-3)
P 		3573
B 9205.1
(1.2)
P 		8678
(0.5)
P 		10415.9
(3)
P 		8071.6
(-0.4)
P 		6543.1
(-2.7)
P 		6314.3
(-3)
P 		4102
C 9682.3
(3)
P 		8843.3
(1.7)
P 		8741.8
(1.5)
P 		8511.8
(1.2)
P 		7748.3
(0)
P 		5799.3
(-3)
P 		3883
avr 9931.3
(3) 		8411.2
(0.4) 		9018.6
(1.4) 		8182.8
(-0) 		7297.4
(-1.6) 		6482.5
(-3) 		3449
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2205
1 2 3 4 range
A 2824
(3)
P 		2768.4
(2.8)
P 		2610.3
(2.3)
P 		908
(-3)
P 		1916
B 2929.4
(3)
P 		2663.6
(2.1)
P 		2339.1
(0.9)
P 		1220.2
(-3)
P 		1709
C 2407.9
(1.1)
P 		3113.1
(3)
P 		2937.3
(2.5)
P 		914.4
(-3)
P 		2199
avr 2720.4
(2.6) 		2848.4
(3) 		2628.9
(2.3) 		1014.2
(-3) 		1834

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1877
1 2 3 4 5 6 range
A 2010.5
(-1.3)
P 		2742.8
(3)
P 		1720
(-3)
P 		2020.1
(-1.2)
P 		2293.6
(0.4)
P 		1785.6
(-2.6)
P 		1023
B 3202.9
(3)
P 		2888.6
(1.2)
P 		2529.3
(-0.8)
P 		2137.9
(-3)
P 		2658.4
(-0.1)
P 		2354.6
(-1.8)
P 		1065
C 3588.8
(3)
P 		2698.4
(0.2)
P 		2224.9
(-1.4)
P 		1711.7
(-3)
P 		2507.2
(-0.5)
P 		1941.4
(-2.3)
P 		1877
avr 2934.1
(3) 		2776.6
(2) 		2158.1
(-1.8) 		1956.6
(-3) 		2486.4
(0.3) 		2027.2
(-2.6) 		978
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192922_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.496
Sequence Type Exemplar sequence
Representative Public ID F26E4.8 NCBI
Target Description F26E4.8 /REP_DB=WormBase Gene ID /WP=CE09692 /GEN=tba-2 /TR=O18688 /GB=CAB03001.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=TBA-2 tubulin alpha-2 chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060199(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060199 NCBI Caenorhabditis elegans TuBulin, Alpha family member (tba-1) (tba-1) mRNA, complete cds. 11/11 None
SNAP00000026230 ENSEMBL cdna:SNAP chromosome:CEL140:I:9794054:9795498:-1 11/11 None
GENEFINDER00000026242 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9794054:9795498:-1 11/11 None
F26E4.8.3 ENSEMBL cdna:known chromosome:CEL140:I:9793899:9795516:-1 gene:F26E4.8 11/11 None
F26E4.8.2 ENSEMBL cdna:known chromosome:CEL140:I:9793899:9795806:-1 gene:F26E4.8 11/11 None
F26E4.8.1 ENSEMBL cdna:known chromosome:CEL140:I:9793899:9795511:-1 gene:F26E4.8 11/11 None
F26E4.8.4 ENSEMBL cdna:known chromosome:CEL140:I:9793899:9795516:-1 gene:F26E4.8 11/11 None
F26E4.8.6 ENSEMBL cdna:known chromosome:CEL140:I:9793900:9795806:-1 gene:F26E4.8 11/11 None
F26E4.8.5 ENSEMBL cdna:known chromosome:CEL140:I:9793900:9795517:-1 gene:F26E4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9793984-9795429(-) 100.0 100.0
Public Domain and Genome References
Gene Title TBA-2 tubulin alpha-2 chain
Gene Symbol tba-1
Ensembl F26E4.8
Entrez Gene 172831 Entrez gene
SwissProt O18688 EMBL-EBI
WormBase CE09692 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262885_AT tubulin alpha-1 chain (TUA1) at
ATGENOME1:17466_S_AT tubulin alpha-1 chain (TUA1) at
CANINE_2:CFAAFFX.23404.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
DROSOPHILA_2:1634906_AT Tubulin at 84D dm
DROSGENOME1:141709_I_AT Tubulin at 84D dm
DROSOPHILA_2:1633624_S_AT Tubulin at 84D dm
DROSGENOME1:141710_R_AT Tubulin at 84D dm
CHICKEN:GGAAFFX.8280.1.S1_AT tubulin, alpha 2 gga
HG-U95AV2:38350_F_AT tubulin, alpha 2 hs
HG-U133A:210527_X_AT tubulin, alpha 2 hs
HG-U133_PLUS_2:210527_X_AT tubulin, alpha 2 hs
HG-U133A_2:210527_X_AT tubulin, alpha 2 hs
HU35KSUBA:RC_AA435720_I_AT tubulin, alpha 2 hs
HU35KSUBD:RC_AA426374_F_AT tubulin, alpha 2 hs
HUGENEFL:K03460_AT tubulin, alpha 2 hs
U133_X3P:G306450_3P_X_AT tubulin, alpha 2 hs
U133_X3P:HS.75318.1.S1_3P_S_AT tubulin, alpha 2 hs
MU11KSUBB:MSA.13449.0_AT similar to tubulin, alpha 2; tubulin alpha 2 mm
MU11KSUBB:MSA.11390.0_AT similar to tubulin, alpha 2; tubulin alpha 2 mm
MU11KSUBB:MSA.10568.0_F_AT similar to tubulin, alpha 2; tubulin alpha 2 mm
MOE430A:1433584_AT similar to tubulin, alpha 2; tubulin alpha 2 mm
MOUSE430A_2:1433584_AT similar to tubulin, alpha 2; tubulin alpha 2 mm
MOUSE430_2:1433584_AT similar to tubulin, alpha 2; tubulin alpha 2 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03001 Hypothetical protein F26E4.8 [Caenorhabditis elegans] ref|NP_492600.1| TuBulin, Alpha family member (tba-1) [Caenorhabditis elegans] 0.0
blast CAE60226 Hypothetical protein CBG03797 [Caenorhabditis briggsae] 0.0
blast BAA03909.1 alpha-1 tubulin [Caenorhabditis elegans] prf||2111240A tubulin:ISOTYPE=alpha 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 2.4E-110
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 8.8E-78
Sequence
Target Sequence 
>C. ELEGANS:192922_S_AT
caaaccaacttggtcccatatccaagaattcatttcccattggctgcgtacactccactg
atctctgctgacaaggcttaccatgaggctctctctgtcaatgacatcaccaacagttgc
ttcgagccagccaaccaaatggtgaagtgcgatccacgccacggaaagtacatggctgtt
tgcctcttgtacagaggagatgtcgttccaaaggacgtcaacactgccatcgccgccatc
aagaccaagagaaccattcaattcgttgactggtgcccaactggattcaaggtcggaatc
aactaccagccaccaactgttgttccaggaggagatctcgccaaggtaccacgtgccgta
tgtatgctctccaacaccaccgccatcgctgaagcctggtctcgcttggactacaagttc
gatcttatgtacgccaagcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAACCAACTTGGTCCCATATCCAA 187 193 772 Antisense
TCCACTGATCTCTGCTGACAAGGCT 371 589 825 Antisense
TTACCATGAGGCTCTCTCTGTCAAT 648 681 849 Antisense
TCACCAACAGTTGCTTCGAGCCAGC 636 625 878 Antisense
AATGGTGAAGTGCGATCCACGCCAC 508 163 909 Antisense
CCAAAGGACGTCAACACTGCCATCG 397 275 979 Antisense
ATTCGTTGACTGGTGCCCAACTGGA 157 9 1032 Antisense
AGGAGGAGATCTCGCCAAGGTACCA 29 55 1098 Antisense
GGTACCACGTGCCGTATGTATGCTC 109 505 1116 Antisense
CCTGGTCTCGCTTGGACTACAAGTT 150 267 1166 Antisense
GTTCGATCTTATGTACGCCAAGCGC 654 441 1188 Antisense
Sequence Source Affymetrix Proprietary Database