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[192921_s_at] serine/threonine kinase

Description Y53C12A.1 /REP_DB=WormBase Gene ID /WP=CE14884 /GEN=wee-1.3 /TR=O18209 /GB=CAB16484.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 97      4grp_total > 32      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 155.1
(-1.5)
A 		140.2
(-3)
A 		199.4
(3)
A 		152.6
(-1.7)
A 		59
B 119.3
(-3)
A 		160.2
(2.3)
A 		136.3
(-0.8)
A 		165.5
(3)
A 		46
C 130.7
(0.8)
A 		44.9
(-3)
A 		179.7
(3)
P 		166.5
(2.4)
A 		135
avr 135
(-0.9) 		115.1
(-3) 		171.8
(3) 		161.5
(1.9) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 775
1 2 3 4 5 6 range
A 160.8
(-2.6)
A 		110.4
(-3)
A 		140.5
(-2.8)
A 		338.2
(-1.1)
P 		552
(0.6)
P 		838.6
(3)
P 		728
B 64
(-3)
A 		115.3
(-2.5)
A 		161.7
(-2)
A 		289.1
(-0.6)
M 		529.8
(1.9)
P 		638
(3)
P 		574
C 105
(-2.9)
A 		92.7
(-3)
A 		165.1
(-2.2)
A 		275.6
(-1)
A 		510.6
(1.6)
P 		637.6
(3)
P 		545
avr 109.9
(-3) 		106.1
(-3) 		155.8
(-2.5) 		301
(-1) 		530.8
(1.3) 		704.7
(3) 		599
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 56.5
(3)
A 		30.4
(-1.6)
A 		52.1
(2.2)
A 		22.3
(-3)
A 		34
B 38.1
(1.9)
A 		39.6
(3)
A 		31.6
(-3)
A 		35.3
(-0.2)
A 		8
C 43.3
(0.7)
A 		12.9
(-3)
A 		62.6
(3)
P 		28.1
(-1.2)
A 		50
avr 46
(2.2) 		27.6
(-3) 		48.8
(3) 		28.6
(-2.7) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 216
1 2 3 4 5 6 range
A 29.7
(-3)
A 		39.3
(-2.7)
A 		30.6
(-3)
A 		85.8
(-1.1)
P 		166.6
(1.7)
P 		204.2
(3)
P 		175
B 22.3
(-3)
A 		38.4
(-2.6)
A 		39.3
(-2.5)
A 		76.6
(-1.5)
M 		215.2
(2.4)
P 		237.9
(3)
P 		216
C 38.9
(-2.7)
A 		28.3
(-3)
A 		42
(-2.6)
A 		55.4
(-2.1)
A 		165.2
(1.4)
P 		213.4
(3)
P 		185
avr 30.3
(-3) 		35.3
(-2.8) 		37.3
(-2.8) 		72.6
(-1.7) 		182.3
(1.8) 		218.5
(3) 		188
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192921_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2771
Sequence Type Exemplar sequence
Representative Public ID Y53C12A.1 NCBI
Target Description Y53C12A.1 /REP_DB=WormBase Gene ID /WP=CE14884 /GEN=wee-1.3 /TR=O18209 /GB=CAB16484.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063694(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063694 NCBI Caenorhabditis elegans WEE homolog family member (wee-1.3) (wee-1.3) mRNA, complete cds. 11/11 None
SNAP00000043900 ENSEMBL cdna:SNAP chromosome:CEL140:II:9714013:9716373:-1 11/11 None
GENEFINDER00000043903 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9714013:9716373:-1 11/11 None
Y53C12A.1 ENSEMBL cdna:known chromosome:CEL140:II:9713648:9716378:-1 gene:Y53C12A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065848 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019671 1/11 Cross Hyb Matching Probes None
SNAP00000019668 1/11 Cross Hyb Matching Probes None
Y37B11A.3 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9714009-9716370(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Gene Symbol wee-1.3
Ensembl Y53C12A.1
Entrez Gene 174531 Entrez gene
SwissProt O18209 EMBL-EBI
WormBase CE14884 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB16484 Hypothetical protein Y53C12A.1 [Caenorhabditis elegans] ref|NP_496095.1| WEE homolog family member (wee-1.3) [Caenorhabditis elegans] sp|O18209|PMYT1_CAEEL Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase (Myt1 kinase) (Lethal protein 37) 0.0
blast CAE57990 Hypothetical protein CBG01053 [Caenorhabditis briggsae] 0.0
blast BAB69973.1 Myt1 [Asterina pectinifera] 4.0E-74
Protein Families
Method ID Description E-Value
hanks 2.1.22 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; RSK2C 3.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-53
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496095.1 1 368-390
Y53C12A.1 1 368-390
SNAP00000043900 1 368-390
GENEFINDER00000043903 1 368-390
Sequence
Target Sequence 
>C. ELEGANS:192921_S_AT
gccgtgtcgcttgaactttgataacgatcaagatgacgatgaggaacaggcgacttgctc
atcatcaaactcttcagctattgagccccaattggatgaaccggagagtccgccgagaat
gaacgatgttattgataaacttggaaaacgaggaactccaagatcagctcgtcgtaatct
cactttcaaccgtcatcgtcaagtagccgcgtcagttgcaccgaaatcgtcgctaaatca
ttataatcatcacactggatctggagatggatttagtaataactctctaattccaattag
tgatcaagaacgaacggaaaaatatctccgaatgcgactcaccgagcaacagttagattg
ggcagatcagaataatgtgatcgacgaggctccaccaccaatgtcttgtccgccaagaat
ccgaagaagcattcgtgatctaccgagaatgcctgttctcaactttaacgttctcgacga
gccatcgaataaaccgactgttgatcatcatacgattttggagcaatcggaatcgccacg
tagaagattaaatagaggggctaagcctgtgcctcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGTGTCGCTTGAACTTTGATAAC 22 285 1428 Antisense
GAGGAACAGGCGACTTGCTCATCAT 314 405 1468 Antisense
GACTTGCTCATCATCAAACTCTTCA 623 371 1479 Antisense
AACTCTTCAGCTATTGAGCCCCAAT 587 139 1495 Antisense
GATGAACCGGAGAGTCCGCCGAGAA 703 411 1522 Antisense
AATGTCTTGTCCGCCAAGAATCCGA 636 163 1827 Antisense
GTGATCTACCGAGAATGCCTGTTCT 614 483 1862 Antisense
GCCTGTTCTCAACTTTAACGTTCTC 411 283 1878 Antisense
TAACGTTCTCGACGAGCCATCGAAT 175 639 1893 Antisense
GAGCAATCGGAATCGCCACGTAGAA 361 391 1948 Antisense
TAGAGGGGCTAAGCCTGTGCCTCGT 540 625 1980 Antisense
Sequence Source Affymetrix Proprietary Database