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[192916_at]

Description C33F10.3 /REP_DB=WormBase Gene ID /WP=CE02530 /CHR=2 /FEA=Sanger Annotation /DEF=E. coli orf_f226; yeast yej4 protein (ST.LOUIS) TR:Q18381 protein_id:AAA93393.1
Cluster 6grp_high1_10_10 > 83      6grp_total > 74      4grp_total > 60      6grp_high1_sub > 04      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 326
1 2 3 4 range
A 535.2
(2.4)
M 		565.3
(3)
A 		525.6
(2.3)
P 		239.4
(-3)
A 		326
B 504.4
(0.1)
A 		540.8
(3)
P 		527.7
(2)
P 		465.4
(-3)
P 		75
C 506.4
(1.9)
A 		473.5
(0.9)
M 		543.6
(3)
P 		339.3
(-3)
P 		204
avr 515.3
(2.4) 		526.5
(2.8) 		532.3
(3) 		348
(-3) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 500.6
(-2.5)
A 		484.1
(-3)
P 		542.2
(-1.1)
P 		668.4
(3)
P 		558.2
(-0.6)
P 		570.9
(-0.2)
P 		184
B 413.4
(-3)
P 		439.9
(-2.3)
P 		651.7
(3)
P 		529.5
(-0.1)
P 		598.6
(1.7)
P 		486.2
(-1.2)
P 		238
C 514.7
(-2.6)
P 		507.9
(-3)
P 		535.3
(-1.4)
P 		523.3
(-2.1)
P 		570
(0.7)
P 		609.1
(3)
P 		101
avr 476.2
(-3) 		477.3
(-2.9) 		576.4
(3) 		573.7
(2.8) 		575.6
(3) 		555.4
(1.7) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 194.9
(3)
M 		122.7
(0.3)
A 		137.4
(0.8)
P 		35
(-3)
A 		160
B 160.9
(3)
A 		133.6
(0.3)
P 		122.3
(-0.8)
P 		99.2
(-3)
P 		62
C 167.6
(2)
A 		136.1
(0.6)
M 		189.5
(3)
P 		57.2
(-3)
P 		132
avr 174.5
(3) 		130.8
(0.6) 		149.7
(1.7) 		63.8
(-3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 151
1 2 3 4 5 6 range
A 92.3
(-3)
A 		172.2
(3)
P 		118.1
(-1.1)
P 		169.5
(2.8)
P 		168.4
(2.7)
P 		139
(0.5)
P 		80
B 143.9
(-2.8)
P 		146.4
(-2.6)
P 		158.3
(-1.9)
P 		140.2
(-3)
P 		243.2
(3)
P 		181.3
(-0.6)
P 		103
C 190.8
(2.2)
P 		155
(0)
P 		136.2
(-1.1)
P 		105.2
(-3)
P 		184.4
(1.8)
P 		203.9
(3)
P 		99
avr 142.3
(-2.5) 		157.9
(-1) 		137.5
(-3) 		138.3
(-2.9) 		198.7
(3) 		174.7
(0.6) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192916_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5657
Sequence Type Exemplar sequence
Representative Public ID C33F10.3 NCBI
Target Description C33F10.3 /REP_DB=WormBase Gene ID /WP=CE02530 /CHR=2 /FEA=Sanger Annotation /DEF=E. coli orf_f226; yeast yej4 protein (ST.LOUIS) TR:Q18381 protein_id:AAA93393.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026840(11), NM_001026842(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026840 NCBI Caenorhabditis elegans C33F10.4a (C33F10.4) mRNA, complete cds. 11/11 None
NM_001026842 NCBI Caenorhabditis elegans C33F10.4c (C33F10.4) mRNA, complete cds. 11/11 None
SNAP00000016465 ENSEMBL cdna:SNAP chromosome:CEL140:II:4797362:4798068:1 11/11 None
GENEFINDER00000016477 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4797362:4798068:1 11/11 None
C33F10.4a ENSEMBL cdna:known chromosome:CEL140:II:4795800:4798103:1 gene:C33F10.4 11/11 None
C33F10.4c ENSEMBL cdna:known chromosome:CEL140:II:4797362:4798068:1 gene:C33F10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173827 3 2 NM_001026840 192916_at (11)
NM_001026841 184960_at (11)
NM_001026842 192916_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4797360-4798067(+) 100.0 100.0
Public Domain and Genome References
Ensembl C33F10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022011 C33F10.4a [Caenorhabditis elegans] gb|AAK31483.1| Hypothetical protein C33F10.4a [Caenorhabditis elegans] 0.0
blast CAE73203 Hypothetical protein CBG20607 [Caenorhabditis briggsae] 1.0E-174
blast NP_001022012 C33F10.4b [Caenorhabditis elegans] gb|AAV58878.1| Hypothetical protein C33F10.4b [Caenorhabditis elegans] pir||T15744 hypothetical protein C33F10.4 - Caenorhabditis elegans 1.0E-117
blast NP_001022013 C33F10.4c [Caenorhabditis elegans] gb|AAV58879.1| Hypothetical protein C33F10.4c [Caenorhabditis elegans] 1.0E-117
blast NP_001022011 C33F10.4a [Caenorhabditis elegans] gb|AAK31483.1| Hypothetical protein C33F10.4a [Caenorhabditis elegans] 1.0E-117
Protein Domains
Database ID Description E-Value
Pfam IPR000534 EMBL-EBI Semialdehyde dehydrogenase 3.7E-8
Pfam IPR000534 EMBL-EBI Semialdehyde dehydrogenase 3.7E-8
Sequence
Target Sequence 
>C. ELEGANS:192916_AT
agctgttggttctgagctcgtcaagctgcttgccgagtctacaaagttttcaaaagtggt
tgttctcgctagaagaccagtcgacggagccactggagacaagctgattcaaaaaactgt
cgactttgataagcttgaagagaacgccgaggatattcaaggagttgacgtggcattctg
tgctttgggaactacacgtggaaagtccggtgctgatggattctacaaggtcgatcacga
ttatgtgatgagcgcggcaaagatggcaaaggaaaatggagtcaaacaattcgtccttgt
tagttccgttggcgccgacgctagttctcgattcttgtacccgaaaaccaagggagaagt
tgaaaaagagatcggagagctcaattttgaaaagttcgtcataatgcgtccaggattgat
tgaagcgaaacgtccagaattccgaatcggagagttcctcggaaaaattgtcactgcacc
actcggacttttctccaatcgtttttcaagttctgccacagccattgctcaagcgatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTGTTGGTTCTGAGCTCGTCAAG 422 79 45 Antisense
GCTCGTCAAGCTGCTTGCCGAGTCT 333 299 60 Antisense
GTTCTCGCTAGAAGACCAGTCGACG 259 443 106 Antisense
GTCGACGGAGCCACTGGAGACAAGC 99 475 124 Antisense
GTTGACGTGGCATTCTGTGCTTTGG 223 439 208 Antisense
CAATTCGTCCTTGTTAGTTCCGTTG 144 173 331 Antisense
CCGACGCTAGTTCTCGATTCTTGTA 7 265 359 Antisense
AAGTTCGTCATAATGCGTCCAGGAT 585 155 436 Antisense
CGGAAAAATTGTCACTGCACCACTC 576 241 504 Antisense
GACTTTTCTCCAATCGTTTTTCAAG 584 371 530 Antisense
GCCACAGCCATTGCTCAAGCGATGA 157 279 559 Antisense
Sequence Source Affymetrix Proprietary Database