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[192915_at] potassium channel protein

Description R04F11.4 /REP_DB=WormBase Gene ID /WP=CE06235 /GEN=twk-15 /TR=Q21729 /GB=CAA98957.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 23      4grp_high_8_8 > 41      6grp_highest_nonorm > 90      6grp_total > 12      4grp_total > 54      6grp_highest_sub > 02      6grp_cgc > 43      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 238
(3)
P 		154.6
(-2)
P 		138.9
(-3)
P 		138.5
(-3)
A 		100
B 294.2
(3)
P 		225.1
(0.4)
P 		166.6
(-1.8)
P 		133.8
(-3)
P 		160
C 254.1
(3)
P 		152.1
(-1.6)
P 		175
(-0.6)
P 		121.6
(-3)
P 		133
avr 262.1
(3) 		177.3
(-0.9) 		160.2
(-1.7) 		131.3
(-3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 139.5
(-2.9)
P 		229.2
(3)
P 		160
(-1.6)
P 		143.5
(-2.7)
P 		138.4
(-3)
P 		142.5
(-2.7)
P 		91
B 221.7
(2.1)
P 		240.8
(3)
P 		217.8
(1.9)
P 		145.7
(-1.6)
P 		123.5
(-2.7)
P 		117.7
(-3)
P 		123
C 194.1
(1)
P 		231.7
(3)
P 		153.8
(-1.1)
P 		148.2
(-1.4)
P 		137.8
(-1.9)
P 		117.1
(-3)
P 		115
avr 185.1
(0.3) 		233.9
(3) 		177.2
(-0.1) 		145.8
(-1.9) 		133.2
(-2.6) 		125.8
(-3) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 86.7
(3)
P 		33.6
(-1.8)
P 		36.3
(-1.6)
P 		20.3
(-3)
A 		66
B 93.8
(3)
P 		55.6
(-0.5)
P 		38.6
(-2.1)
P 		28.5
(-3)
P 		65
C 84.1
(3)
P 		43.7
(-0.8)
P 		61
(0.8)
P 		20.5
(-3)
P 		64
avr 88.2
(3) 		44.3
(-1) 		45.3
(-1) 		23.1
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 25.7
(-3)
P 		81.5
(3)
P 		34.8
(-2)
P 		36.4
(-1.8)
P 		41.8
(-1.3)
P 		34.7
(-2)
P 		56
B 77.1
(2.6)
P 		80.2
(3)
P 		52.9
(-0.9)
P 		38.6
(-3)
P 		50.2
(-1.3)
P 		43.9
(-2.2)
P 		42
C 71.9
(3)
P 		70.7
(2.8)
P 		39.1
(-1.7)
P 		29.8
(-3)
P 		44.6
(-0.9)
P 		39.2
(-1.7)
P 		42
avr 58.2
(0.3) 		77.5
(3) 		42.3
(-2) 		34.9
(-3) 		45.5
(-1.5) 		39.3
(-2.4) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192915_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13524
Sequence Type Exemplar sequence
Representative Public ID R04F11.4 NCBI
Target Description R04F11.4 /REP_DB=WormBase Gene ID /WP=CE06235 /GEN=twk-15 /TR=Q21729 /GB=CAA98957.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073690(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073690 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-13) (twk-13) mRNA, complete cds. 11/11 None
SNAP00000007841 ENSEMBL cdna:SNAP chromosome:CEL140:V:12323148:12327197:-1 11/11 None
GENEFINDER00000007845 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12323148:12331018:-1 11/11 None
R04F11.4 ENSEMBL cdna:known chromosome:CEL140:V:12322775:12334777:-1 gene:R04F11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12323147-12327827(-) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein
Gene Symbol twk-13
Ensembl R04F11.4
Entrez Gene 192077 Entrez gene
SwissProt Q21729 EMBL-EBI
WormBase CE30561 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98957 Hypothetical protein R04F11.4 [Caenorhabditis elegans] ref|NP_506091.2| TWiK family of potassium channels family member (twk-13) [Caenorhabditis elegans] 0.0
blast CAE75458 Hypothetical protein CBG23453 [Caenorhabditis briggsae] 0.0
blast CAA92504 Hypothetical protein ZK1251.8 [Caenorhabditis elegans] ref|NP_501724.1| TWiK family of potassium channels family member (twk-9) [Caenorhabditis elegans] sp|Q23435|TWK9_CAEEL TWiK family of potassium channels protein 1.0E-29
blast AAU87822 Twik family of potassium channels protein 7 [Caenorhabditis elegans] ref|NP_498903.3| TWiK family of potassium channels family member (twk-7) [Caenorhabditis elegans] sp|P34410|TWK7_CAEEL TWiK family of potassium channels protein 2.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 1.0E-10
Pfam IPR005821 EMBL-EBI Ion transport protein 1.0E-10
Pfam IPR005821 EMBL-EBI Ion transport protein 1.0E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506091.2 7 51-73,157-179,188-207,222-244,338-360,364-386,393-415
R04F11.4 7 51-73,157-179,188-207,222-244,338-360,364-386,393-415
SNAP00000007841 5 31-53,63-85,212-234,238-260,267-289
GENEFINDER00000007845 6 112-134,215-237,247-269,396-418,422-444,451-473
Sequence
Target Sequence 
>C. ELEGANS:192915_AT
gggtttggtgacattgtacctgcaaatgttgattggcttccagcaacacttgcatatatt
gtctttggactcataatcacaactatgtgcattgacttggtcggaagtgaatatatccga
gatattcatttttacggacgaagtcttggtcgtcaattcatgacaattggtggaaaagtg
gtacatcttggagaagtatttggatacgtggcattccttcaaaagaattatggattgact
gctgagcaacttacaaaattatctcaattaccagaagaatatctattagattgtctgata
aatggaagacagcctgatttgaattggattggaggacgaccatatgtaccaccagatata
tattatttcaaatggattgaacatccaaggacactttcatttgcatctgatcgagttttg
cagagtatggagagtcttgatttgaatactagcagatgctccaccgctcgaacattgaca
cctcgagaatattatcagaagattcttttccagtactgtaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTTTGGTGACATTGTACCTGCAA 206 495 1024 Antisense
TTGATTGGCTTCCAGCAACACTTGC 17 705 1052 Antisense
ACTTGCATATATTGTCTTTGGACTC 567 101 1071 Antisense
AACTATGTGCATTGACTTGGTCGGA 202 139 1104 Antisense
GAAGTCTTGGTCGTCAATTCATGAC 688 335 1163 Antisense
ATTTGGATACGTGGCATTCCTTCAA 248 19 1221 Antisense
GACGACCATATGTACCACCAGATAT 91 379 1358 Antisense
AACATCCAAGGACACTTTCATTTGC 579 137 1403 Antisense
CACTTTCATTTGCATCTGATCGAGT 472 197 1415 Antisense
ACCGCTCGAACATTGACACCTCGAG 654 87 1486 Antisense
AGAAGATTCTTTTCCAGTACTGTAA 126 75 1520 Antisense
Sequence Source Affymetrix Proprietary Database