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[192914_at] dihydropyrimidinase

Description R06C7.3 /REP_DB=WormBase Gene ID /WP=CE28484 /GEN=dhp-1 /TR=SW:Q21773 /GB=CAA95842.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Rat TOAD-64 protein like
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 56      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 85      6grp_total > 46      4grp_total > 10      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2992
1 2 3 4 range
A 1147.8
(-2.8)
P 		1216.8
(-2.7)
P 		1067.5
(-3)
P 		3847
(3)
P 		2780
B 1190.6
(-2.9)
P 		1476.4
(-2.3)
P 		1138.9
(-3)
P 		4059.1
(3)
P 		2920
C 1255.7
(-3)
P 		1408
(-2.6)
P 		1272.1
(-3)
P 		3286.6
(3)
P 		2031
avr 1198
(-2.9) 		1367.1
(-2.5) 		1159.5
(-3) 		3730.9
(3) 		2571

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 830
1 2 3 4 5 6 range
A 726.4
(-0.6)
P 		810.9
(0.2)
P 		629.3
(-1.5)
P 		503.1
(-2.8)
P 		480.8
(-3)
P 		1090.6
(3)
P 		610
B 1051.5
(1.6)
P 		834.4
(-0.1)
P 		938.1
(0.7)
P 		518
(-2.5)
P 		452.5
(-3)
P 		1239.8
(3)
P 		787
C 956
(1.2)
P 		936.8
(1)
P 		728.8
(-0.6)
P 		548.6
(-1.9)
P 		410.2
(-3)
P 		1191.4
(3)
P 		781
avr 911.3
(0.8) 		860.7
(0.4) 		765.4
(-0.4) 		523.2
(-2.4) 		447.8
(-3) 		1173.9
(3) 		726
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 601
1 2 3 4 range
A 417.9
(0.1)
P 		264.1
(-3)
P 		279.1
(-2.7)
P 		563.1
(3)
P 		299
B 379.7
(-1.8)
P 		364.6
(-2)
P 		263.9
(-3)
P 		865
(3)
P 		601
C 415.7
(-2.6)
P 		404.8
(-3)
P 		443.4
(-1.4)
P 		554.2
(3)
P 		149
avr 404.4
(-1.6) 		344.5
(-2.7) 		328.8
(-3) 		660.8
(3) 		332

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 352
1 2 3 4 5 6 range
A 133.9
(-2.8)
P 		288.4
(3)
P 		137.1
(-2.6)
P 		127.6
(-3)
P 		145.1
(-2.3)
P 		265.5
(2.1)
P 		161
B 365.9
(1.2)
P 		277.7
(-0.4)
P 		227.8
(-1.3)
P 		137.2
(-3)
P 		183.8
(-2.1)
P 		462.3
(3)
P 		325
C 354.3
(2.1)
P 		285.8
(0.6)
P 		185.5
(-1.4)
P 		110.3
(-3)
P 		132.7
(-2.5)
P 		398.8
(3)
P 		289
avr 284.7
(0.8) 		284
(0.8) 		183.5
(-1.6) 		125
(-3) 		153.9
(-2.3) 		375.5
(3) 		251
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192914_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1896
Sequence Type Exemplar sequence
Representative Public ID R06C7.3 NCBI
Target Description R06C7.3 /REP_DB=WormBase Gene ID /WP=CE28484 /GEN=dhp-1 /TR=SW:Q21773 /GB=CAA95842.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Rat TOAD-64 protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026412(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026412 NCBI Caenorhabditis elegans R06C7.3 (dihydropyrimidinase) mRNA, complete cds. 11/11 None
GENEFINDER00000036121 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7247215:7251884:-1 11/11 None
SNAP00000036111 ENSEMBL cdna:SNAP chromosome:CEL140:I:7248371:7251884:-1 9/11 None
R06C7.3.1 ENSEMBL cdna:known chromosome:CEL140:I:7247125:7251894:-1 gene:R06C7.3 11/11 None
R06C7.3.2 ENSEMBL cdna:known chromosome:CEL140:I:7247215:7252098:-1 gene:R06C7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7248301-7252052(-) 100.0 100.0
Public Domain and Genome References
Gene Title dihydropyrimidinase
Gene Symbol dhp-1
Ensembl R06C7.3
Entrez Gene 172464 Entrez gene
SwissProt Q21773 EMBL-EBI
WormBase CE30293 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6208 pyrimidine base catabolism inferred from direct assay QuickGO AmiGO
46113 nucleobase catabolism inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
4157 dihydropyrimidinase activity inferred from sequence similarity QuickGO AmiGO
16812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD24483 Hypothetical protein R06C7.3 [Caenorhabditis elegans] ref|NP_001021583.1| R06C7.3 [Caenorhabditis elegans] sp|Q21773|DHP1_CAEEL Dihydropyrimidinase 1 (CeCRMP/DHP-1) (UlipB) dbj|BAB21560.1| CeCRMP/DHP-1 [Caenorhabditis elegans] 0.0
blast Q60Q85 Dihydropyrimidinase 1 emb|CAE74237.1| Hypothetical protein CBG21922 [Caenorhabditis briggsae] 0.0
blast T23968 hypothetical protein R06C7.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-10
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.3E-10
Pfam IPR006680 EMBL-EBI Amidohydrolase 3.6E-22
Pfam IPR006680 EMBL-EBI Amidohydrolase 6.7E-22
Sequence
Target Sequence 
>C. ELEGANS:192914_AT
gttttggctgctcaagctaattgtccagtttatatcgttcatgtaatgacaaaaggagcc
gcatcagcaatttcccatcaccgagctcaaggttccattgtttttggagagccaatagct
gctggattagctcttgatggatcacattattataatgaagattggctccatgctgcaaga
tatgtcatgtctccaccattaagcagagatccaactacacctgaattacttatgaaatta
cttgcggccggggaacttcacctgacaggaacagacaattgcacatatgattgcaggcaa
aaatctcttggaaaaggaaacttcacaaaaattccaaatggaatcaatggtgtcgaggat
cgaatgtcagttgtatgggaaaaaggggtacattctggaataattgatccaatgagatat
gtctcaataaccagttcaacagctgcaaaaatttttaatatttatccaagaaagggaaga
attgcagttggttccgatgcagatattgtaattttcaatccaaatgccactagaactatt
tcaaaggacacacatcatcataacctcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTGGCTGCTCAAGCTAATTGTC 369 447 1018 Antisense
AATTGTCCAGTTTATATCGTTCATG 488 179 1036 Antisense
AAAGGAGCCGCATCAGCAATTTCCC 580 121 1069 Antisense
CCATCACCGAGCTCAAGGTTCCATT 90 271 1092 Antisense
GCTGGATTAGCTCTTGATGGATCAC 256 295 1138 Antisense
GAAGATTGGCTCCATGCTGCAAGAT 609 337 1174 Antisense
ATATGTCATGTCTCCACCATTAAGC 405 21 1197 Antisense
GAGATCCAACTACACCTGAATTACT 137 397 1223 Antisense
TGAAATTACTTGCGGCCGGGGAACT 259 571 1250 Antisense
CGGGGAACTTCACCTGACAGGAACA 651 241 1266 Antisense
GGACACACATCATCATAACCTCGAT 254 527 1563 Antisense
Sequence Source Affymetrix Proprietary Database