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[192893_at]

Description R153.1 /REP_DB=WormBase Gene ID /WP=CE02038 /TR=SW:Q22000 /GB=AAA68292.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=cyclic nucleotide phosphodiesterase
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 04      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 51      6grp_total > 25      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 716
1 2 3 4 range
A 1057.4
(3)
P 		455.5
(-2)
P 		341.4
(-3)
P 		581.9
(-1)
P 		716
B 978.3
(3)
P 		481.5
(-2)
P 		385.6
(-3)
P 		487.1
(-2)
P 		593
C 924.5
(3)
P 		606.9
(-1.4)
P 		487.2
(-3)
P 		612.8
(-1.3)
P 		437
avr 986.7
(3) 		514.6
(-1.9) 		404.7
(-3) 		560.6
(-1.4) 		582

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 516
1 2 3 4 5 6 range
A 879.5
(3)
P 		688.4
(0.7)
P 		590.3
(-0.5)
P 		407.4
(-2.8)
P 		435.6
(-2.4)
P 		387.1
(-3)
P 		492
B 693.4
(1.8)
P 		778
(3)
P 		567.6
(-0)
P 		363.3
(-3)
P 		498.2
(-1)
P 		459.9
(-1.6)
P 		415
C 628.4
(1.6)
P 		702.1
(3)
P 		545.6
(-0.1)
P 		395.7
(-3)
P 		467.1
(-1.6)
P 		476.7
(-1.4)
P 		306
avr 733.8
(3) 		722.8
(2.8) 		567.8
(0.1) 		388.8
(-3) 		467
(-1.6) 		441.2
(-2.1) 		345
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 300
1 2 3 4 range
A 385
(3)
P 		98.9
(-2.7)
P 		89.3
(-2.9)
P 		85.2
(-3)
P 		300
B 312
(3)
P 		118.9
(-2.2)
P 		89.3
(-3)
P 		103.8
(-2.6)
P 		223
C 306
(3)
P 		174.5
(-0.9)
P 		169.8
(-1)
P 		103.3
(-3)
P 		203
avr 334.3
(3) 		130.8
(-2.2) 		116.1
(-2.5) 		97.4
(-3) 		237

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 162.1
(-0.3)
P 		244.8
(3)
P 		128.6
(-1.6)
P 		103.3
(-2.6)
P 		131.4
(-1.5)
P 		94.2
(-3)
P 		151
B 241.3
(2.4)
P 		258.9
(3)
P 		137.8
(-1.5)
P 		96.2
(-3)
P 		202.4
(0.9)
P 		171.5
(-0.2)
P 		163
C 232.9
(3)
P 		214.2
(2.3)
P 		138.9
(-0.7)
P 		79.6
(-3)
P 		151.2
(-0.2)
P 		159.6
(0.1)
P 		153
avr 212.1
(1.9) 		239.3
(3) 		135.1
(-1.3) 		93
(-3) 		161.7
(-0.2) 		141.8
(-1) 		146
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192893_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5702
Sequence Type Exemplar sequence
Representative Public ID R153.1 NCBI
Target Description R153.1 /REP_DB=WormBase Gene ID /WP=CE02038 /TR=SW:Q22000 /GB=AAA68292.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=cyclic nucleotide phosphodiesterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063199(11), NM_182146(11), NM_182144(11), NM_182145(11), NM_063200(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063199 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-4) (pde-4) mRNA, complete cds. 11/11 None
NM_182146 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-4) (pde-4) mRNA, complete cds. 11/11 None
NM_182144 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-4) (pde-4) mRNA, complete cds. 11/11 None
NM_182145 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-4) (pde-4) mRNA, complete cds. 11/11 None
NM_063200 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-4) (pde-4) mRNA, complete cds. 11/11 None
SNAP00000035257 ENSEMBL cdna:SNAP chromosome:CEL140:II:7627986:7630936:-1 11/11 None
GENEFINDER00000035261 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7627986:7635664:-1 11/11 None
R153.1b ENSEMBL cdna:known chromosome:CEL140:II:7627174:7649877:-1 gene:R153.1 11/11 A 
R153.1c ENSEMBL cdna:known chromosome:CEL140:II:7627986:7643072:-1 gene:R153.1 11/11 None
R153.1e ENSEMBL cdna:known chromosome:CEL140:II:7627986:7649907:-1 gene:R153.1 11/11 None
R153.1d ENSEMBL cdna:known chromosome:CEL140:II:7627986:7642081:-1 gene:R153.1 11/11 None
R153.1a ENSEMBL cdna:known chromosome:CEL140:II:7627986:7634153:-1 gene:R153.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174235 6 5 NM_063199 192893_at (11), 194175_x_at (11)
NM_063200 192893_at (11)
NM_182144 192893_at (11)
NM_182145 192893_at (11)
NM_182146 192893_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7627982-7634150(-) 100.0 100.0
Public Domain and Genome References
Ensembl R153.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK77625 Hypothetical protein R153.1b [Caenorhabditis elegans] ref|NP_495600.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] 0.0
blast AAO38608 Hypothetical protein R153.1e [Caenorhabditis elegans] ref|NP_871946.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] 0.0
blast AAO38607 Hypothetical protein R153.1d [Caenorhabditis elegans] ref|NP_871945.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] sp|Q22000|PDE4_CAEEL Probable 3',5'-cyclic phosphodiesterase pde-4 0.0
blast AAN60496 Hypothetical protein R153.1c [Caenorhabditis elegans] ref|NP_871944.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192893_AT
gaaagtgtccttgagcagcatcatctggcagttgcattcaagcttttgcaagattcaaac
tgtgatttccttgcaaatttgtccagaaaacaacggctgcaatttcgcaagattgtcatt
gatatggtacttgcaactgatatgtctaagcacatgtctcttcttgctgatctgaaaaca
atggtcgaagcaaagaaagttgctggaaacaatgtgattgtgctggacaagtacaatgac
aaaattcaggtactacaatcgatgattcatctcgctgatctctcaaatccaactaagcca
atcgaattataccaacaatggaatcaaaggataatggaagaatactggaggcagggagat
aaagagaaagagcttggacttgagatatcaccgatgtgcgatcgaggaaatgtgaccatt
gaaaaatcacaggtcggattcatcgactacattgttcatccattatacgaaacatgggca
gatcttgtctatccagatgctcaaaatatactcgatcaactcgaagaaaatcgagaatgg
taccaatcacgaatacctgaagaacctgacacagctcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGTGTCCTTGAGCAGCATCATC 418 361 1054 Antisense
GCAGCATCATCTGGCAGTTGCATTC 356 313 1068 Antisense
GTGATTTCCTTGCAAATTTGTCCAG 137 485 1115 Antisense
ACAACGGCTGCAATTTCGCAAGATT 243 113 1143 Antisense
TGTCTAAGCACATGTCTCTTCTTGC 175 559 1196 Antisense
GTCTCTTCTTGCTGATCTGAAAACA 511 469 1209 Antisense
TCGATGATTCATCTCGCTGATCTCT 118 603 1312 Antisense
CTCGCTGATCTCTCAAATCCAACTA 240 233 1324 Antisense
GGACTTGAGATATCACCGATGTGCG 468 525 1429 Antisense
GCAGATCTTGTCTATCCAGATGCTC 240 313 1531 Antisense
ACCTGAAGAACCTGACACAGCTCGT 199 89 1608 Antisense
Sequence Source Affymetrix Proprietary Database