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[192892_s_at] NADH:CoQ oxidoreductase subunit B18

Description D2030.4 /REP_DB=WormBase Gene ID /WP=CE09081 /TR=SW:P90789 /GB=CAA98116.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH:CoQ oxidoreductase subunit B18
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 41      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 16      6grp_total > 41      4grp_total > 49      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2243
1 2 3 4 range
A 6030
(-2.8)
P 		6559.9
(-0.7)
P 		7486.7
(3)
P 		5981.6
(-3)
P 		1505
B 5413.9
(-3)
P 		7656.5
(3)
P 		7284.7
(2)
P 		6509
(-0.1)
P 		2243
C 6374.5
(-0.8)
P 		7503.7
(3)
P 		7218.2
(2.1)
P 		5697.8
(-3)
P 		1806
avr 5939.5
(-3) 		7240
(2.6) 		7329.9
(3) 		6062.8
(-2.5) 		1390

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6541
1 2 3 4 5 6 range
A 5117
(-1.6)
P 		7050.2
(0.1)
P 		10168.1
(3)
P 		8060
(1.1)
P 		5018.6
(-1.7)
P 		3627
(-3)
P 		6541
B 6557.3
(0.1)
P 		7772.7
(1.6)
P 		8919.3
(3)
P 		7170.2
(0.9)
P 		6479.1
(0)
P 		3992.7
(-3)
P 		4927
C 6607.6
(1)
P 		7066.2
(1.7)
P 		7862.6
(3)
P 		7372.4
(2.2)
P 		5788.1
(-0.3)
P 		4126
(-3)
P 		3737
avr 6094
(-0.4) 		7296.4
(1) 		8983.3
(3) 		7534.2
(1.3) 		5761.9
(-0.8) 		3915.2
(-3) 		5068
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1640
1 2 3 4 range
A 2195.5
(3)
P 		1424
(-0.5)
P 		1957.2
(1.9)
P 		875.6
(-3)
P 		1320
B 1726.4
(1)
P 		1891
(3)
P 		1688
(0.6)
P 		1387.1
(-3)
P 		504
C 2110.1
(1.4)
P 		2157.4
(1.6)
P 		2515.8
(3)
P 		960.7
(-3)
P 		1555
avr 2010.7
(2.7) 		1824.1
(1.6) 		2053.7
(3) 		1074.5
(-3) 		979

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1749
1 2 3 4 5 6 range
A 943.3
(-2.8)
P 		2507.4
(3)
P 		2214.4
(1.9)
P 		2044.2
(1.3)
P 		1514.4
(-0.7)
P 		883.1
(-3)
P 		1624
B 2281.6
(1.2)
P 		2587.2
(2.8)
P 		2165.9
(0.6)
P 		1899.1
(-0.8)
P 		2632.4
(3)
P 		1488.9
(-3)
P 		1144
C 2449.1
(3)
P 		2156.1
(1.4)
P 		2001.2
(0.5)
P 		1482.6
(-2.4)
P 		1872.9
(-0.2)
P 		1381.2
(-3)
P 		1068
avr 1891.3
(0.3) 		2416.9
(3) 		2127.2
(1.5) 		1808.6
(-0.1) 		2006.6
(0.9) 		1251.1
(-3) 		1166
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192892_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.428
Sequence Type Exemplar sequence
Representative Public ID D2030.4 NCBI
Target Description D2030.4 /REP_DB=WormBase Gene ID /WP=CE09081 /TR=SW:P90789 /GB=CAA98116.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH:CoQ oxidoreductase subunit B18
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059718(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059718 NCBI Caenorhabditis elegans D2030.4 (D2030.4) mRNA, complete cds. 11/11 None
SNAP00000031273 ENSEMBL cdna:SNAP chromosome:CEL140:I:7589192:7589750:-1 11/11 None
GENEFINDER00000031286 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7589192:7589750:-1 11/11 None
D2030.4.2 ENSEMBL cdna:known chromosome:CEL140:I:7589072:7589755:-1 gene:D2030.4 11/11 None
D2030.4.1 ENSEMBL cdna:known chromosome:CEL140:I:7589081:7589757:-1 gene:D2030.4 11/11 None
D2030.4.3 ENSEMBL cdna:known chromosome:CEL140:I:7589083:7589761:-1 gene:D2030.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7589122-7589681(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADH:CoQ oxidoreductase subunit B18
Ensembl D2030.4
Entrez Gene 172513 Entrez gene
SwissProt P90789 EMBL-EBI
WormBase CE09081 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1588757_AT similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 cfa
CANINE_2:CFAAFFX.24925.1.S1_AT similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 cfa
DROSOPHILA_2:1637018_AT dm
DROSGENOME1:145097_AT dm
HG-U133_PLUS_2:202839_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U133A:202839_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-FOCUS:202839_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U133A_2:202839_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U133_PLUS_2:211407_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U133A:211407_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U133A_2:211407_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HU35KSUBD:RC_AA609299_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HUGENEFL:M33374_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
U133_X3P:G180232_3P_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
U133_X3P:G10764846_3P_X_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
U133_X3P:G10764846_3P_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U95AV2:35773_I_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
HG-U95AV2:35774_R_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa hs
MOE430A:1416417_A_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MOUSE430A_2:1416417_A_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MOUSE430_2:1416417_A_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MU11KSUBA:AA271599_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MG-U74AV2:95698_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MG-U74AV2:162323_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MOE430A:1448331_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MOUSE430A_2:1448331_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
MOUSE430_2:1448331_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 mm
RG-U34B:RC_AA850130_G_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RG-U34B:RC_AA850130_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RG-U34B:RC_AA958010_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RAE230A:1388343_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RAT230_2:1388343_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RAE230B:1382367_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RAT230_2:1382367_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
RG-U34C:RC_AI228502_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5739 mitochondrion inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3954 NADH dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
8137 NADH dehydrogenase (ubiquinone) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98116 Hypothetical protein D2030.4 [Caenorhabditis elegans] ref|NP_492119.1| D2030.4 [Caenorhabditis elegans] sp|P90789|NB8M_CAEEL Probable NADH-ubiquinone oxidoreductase B18 subunit (Complex I-B18) (CI-B18) 1.0E-58
blast CAE66624 Hypothetical protein CBG11960 [Caenorhabditis briggsae] 3.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR008698 EMBL-EBI NADH-ubiquinone oxidoreductase B18 subunit 4.3E-98
Sequence
Target Sequence 
>C. ELEGANS:192892_S_AT
gggaactaaactgtccgtctcattggaaggagccagtacaccagaaaccgcgccacgtgt
cgacagaccaccaactttcgatcctcagtatggattcgagcgaccgagaaaggtccgcga
gatgaaggctacttgggaagagatggagcagtggaagttgaagccagctcaacgcgatta
ttgtgctcaccacttgatttcactcatgaaatgccaaacccaaaatgcaccatttgccgg
acacgcgtgcgacggagaacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGAACTAAACTGTCCGTCTCATTG 421 493 15 Antisense
TGTCCGTCTCATTGGAAGGAGCCAG 40 557 26 Antisense
AAGGAGCCAGTACACCAGAAACCGC 250 159 41 Antisense
TGTCGACAGACCACCAACTTTCGAT 512 557 72 Antisense
CACCAACTTTCGATCCTCAGTATGG 696 199 83 Antisense
CTCAGTATGGATTCGAGCGACCGAG 570 227 98 Antisense
AAAGGTCCGCGAGATGAAGGCTACT 353 119 123 Antisense
GCTCAACGCGATTATTGTGCTCACC 64 303 181 Antisense
TGTGCTCACCACTTGATTTCACTCA 384 557 196 Antisense
CAAAATGCACCATTTGCCGGACACG 666 189 235 Antisense
CGGACACGCGTGCGACGGAGAACGT 315 243 252 Antisense
Sequence Source Affymetrix Proprietary Database