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[192884_at] potassium channel protein

Description ZK1251.8 /REP_DB=WormBase Gene ID /WP=CE03855 /GEN=twk-21 /TR=Q23435 /GB=CAA92504.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_high1_10_10 > 10      4grp_high_8_8 > 24      6grp_total > 50      4grp_total > 55      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 85.8
(3)
P 		34.3
(-3)
P 		44.5
(-1.8)
P 		42.1
(-2.1)
A 		52
B 124
(3)
P 		42.8
(-3)
P 		91
(0.6)
P 		68.7
(-1.1)
P 		81
C 99.6
(3)
P 		44.6
(-3)
P 		60.6
(-1.2)
P 		44.3
(-3)
P 		55
avr 103.1
(3) 		40.6
(-3) 		65.4
(-0.6) 		51.7
(-1.9) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 111.3
(2.9)
P 		84.3
(0.9)
P 		94.3
(1.6)
P 		112.1
(3)
P 		34.1
(-3)
P 		49.3
(-1.8)
A 		78
B 89.3
(2.7)
P 		71.5
(0.3)
P 		86.6
(2.4)
P 		91.3
(3)
P 		86
(2.3)
P 		47.6
(-3)
P 		44
C 77.6
(1.2)
P 		90.6
(2.3)
P 		93.5
(2.6)
P 		97.9
(3)
P 		59.1
(-0.5)
P 		31
(-3)
P 		67
avr 92.7
(2.2) 		82.1
(1.1) 		91.5
(2.1) 		100.4
(3) 		59.7
(-1.2) 		42.6
(-3) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 31.3
(3)
P 		7.5
(-2.7)
P 		11.6
(-1.7)
P 		6.2
(-3)
A 		25
B 39.6
(3)
P 		10.6
(-3)
P 		21.1
(-0.8)
P 		14.6
(-2.2)
P 		29
C 33
(3)
P 		12.8
(-1.8)
P 		21.1
(0.2)
P 		7.5
(-3)
P 		26
avr 34.6
(3) 		10.3
(-2.8) 		17.9
(-1) 		9.4
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 20.5
(0.1)
P 		30
(3)
P 		20.5
(0.1)
P 		28.4
(2.5)
P 		10.3
(-3)
P 		12
(-2.5)
A 		20
B 31.1
(1.7)
P 		23.8
(-0.9)
P 		21
(-1.8)
P 		24.2
(-0.7)
P 		34.9
(3)
P 		17.7
(-3)
P 		17
C 28.8
(3)
P 		27.7
(2.6)
P 		23.8
(1.4)
P 		19.7
(0)
P 		19.1
(-0.2)
P 		10.4
(-3)
P 		18
avr 26.8
(2.8) 		27.2
(3) 		21.8
(0.7) 		24.1
(1.7) 		21.4
(0.5) 		13.4
(-3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192884_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10032
Sequence Type Exemplar sequence
Representative Public ID ZK1251.8 NCBI
Target Description ZK1251.8 /REP_DB=WormBase Gene ID /WP=CE03855 /GEN=twk-21 /TR=Q23435 /GB=CAA92504.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069323(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069323 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-9) (twk-9) mRNA, complete cds. 11/11 None
SNAP00000023388 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9693601:9696950:1 11/11 None
GENEFINDER00000023391 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9693179:9696832:1 11/11 None
ZK1251.8 ENSEMBL cdna:known chromosome:CEL140:IV:9693179:9696208:1 gene:ZK1251.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9693184-9696214(+) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein
Gene Symbol twk-9
Ensembl ZK1251.8
Entrez Gene 177803 Entrez gene
SwissProt Q23435 EMBL-EBI
WormBase CE03855 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92504 Hypothetical protein ZK1251.8 [Caenorhabditis elegans] ref|NP_501724.1| TWiK family of potassium channels family member (twk-9) [Caenorhabditis elegans] sp|Q23435|TWK9_CAEEL TWiK family of potassium channels protein 0.0
blast CAE74823 Hypothetical protein CBG22661 [Caenorhabditis briggsae] 0.0
blast CAE74656 Hypothetical protein CBG22456 [Caenorhabditis briggsae] 3.0E-71
blast CAE74656 Hypothetical protein CBG22456 [Caenorhabditis briggsae] 1.0E-70
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501724.1 6 15-37,161-183,193-215,317-336,346-363,370-392
ZK1251.8 6 15-37,161-183,193-215,317-336,346-363,370-392
SNAP00000023388 6 15-37,161-183,193-215,317-336,346-363,370-392
GENEFINDER00000023391 6 15-37,161-183,193-215,317-336,346-363,370-392
Sequence
Target Sequence 
>C. ELEGANS:192884_AT
gagaactgctagagaacctggagaaggtgatgaaattgaagtaattgaaaatggaaatga
tgagaatggaaaagaagaagatgaggaggagccggagaataatgaaccgagaaaaacgga
agaatcaatagctcttggaataacattcacatgctatctcgtggccggagcaaaaatttt
atcagtttacgagccagaaatggactttttcaaagctttatacttcaattttgtgacact
taccaccattggactgggagattttgtaccgaaaagtttcgattatcttctcattactct
aatttacattggaattggcttggctctgactacaatggcaattgaaattgcagctgattt
attgaaaaagttgcattatattggaagaaaaatggaaaatgttggtcaggcagttgtttg
gtttggaggaaaaaaaatgaccatgaaatcattggttaaacatttgggagatcaattcaa
cattccagaagaagaacttgcaaatttcgatatgtcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAACTGCTAGAGAACCTGGAGAA 515 391 834 Antisense
AACATTCACATGCTATCTCGTGGCC 195 139 975 Antisense
GCTATCTCGTGGCCGGAGCAAAAAT 686 303 986 Antisense
AATTTTATCAGTTTACGAGCCAGAA 372 175 1008 Antisense
ATACTTCAATTTTGTGACACTTACC 684 21 1053 Antisense
GACACTTACCACCATTGGACTGGGA 323 365 1068 Antisense
GTTTCGATTATCTTCTCATTACTCT 419 451 1109 Antisense
TTACATTGGAATTGGCTTGGCTCTG 654 681 1137 Antisense
GGCTTGGCTCTGACTACAATGGCAA 477 537 1150 Antisense
GTTGGTCAGGCAGTTGTTTGGTTTG 60 437 1234 Antisense
AACTTGCAAATTTCGATATGTCAGC 396 139 1328 Antisense
Sequence Source Affymetrix Proprietary Database