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[192880_at] G-protein coupled receptor

Description C39E6.6 /REP_DB=WormBase Gene ID /WP=CE06941 /GEN=npr-1 /TR=Q18534 /GB=AAA93419.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_total > 24      4grp_total > 22      6grp_cgc > 33      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 216
1 2 3 4 range
A 183.2
(2.2)
P 		51
(-3)
A 		64.5
(-2.5)
A 		204.1
(3)
P 		153
B 266.7
(3)
P 		94
(-2.4)
A 		75
(-3)
A 		183.1
(0.4)
P 		192
C 189.3
(1.1)
P 		96.8
(-2.6)
A 		87.3
(-3)
A 		234.9
(3)
P 		148
avr 213.1
(3) 		80.6
(-2.8) 		75.6
(-3) 		207.4
(2.8) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 90.8
(-0.7)
P 		145.6
(3)
P 		135.2
(2.3)
P 		71.9
(-1.9)
A 		61
(-2.7)
A 		56
(-3)
A 		90
B 201.7
(3)
P 		168.5
(1.6)
P 		85
(-2.1)
A 		95.2
(-1.6)
A 		76.5
(-2.4)
P 		63.2
(-3)
P 		139
C 156.1
(2.8)
P 		158.6
(3)
P 		145.8
(2.2)
P 		102.8
(-0.5)
A 		62.6
(-3)
A 		64.3
(-2.9)
P 		96
avr 149.5
(2.5) 		157.6
(3) 		122
(0.8) 		90
(-1.2) 		66.7
(-2.7) 		61.2
(-3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 66.7
(3)
P 		11.1
(-3)
A 		16.9
(-2.4)
A 		29.9
(-1)
P 		56
B 85
(3)
P 		23.2
(-2.5)
A 		17.4
(-3)
A 		39
(-1.1)
P 		68
C 62.7
(3)
P 		27.8
(-3)
A 		30.4
(-2.6)
A 		39.6
(-1)
P 		35
avr 71.5
(3) 		20.7
(-3) 		21.6
(-2.9) 		36.2
(-1.2) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 16.7
(-2.5)
P 		51.8
(3)
P 		29.4
(-0.5)
P 		18.2
(-2.3)
A 		18.4
(-2.2)
A 		13.6
(-3)
A 		38
B 70.2
(3)
P 		56.1
(1.3)
P 		20.6
(-3)
A 		25.2
(-2.4)
A 		31.1
(-1.7)
P 		23.6
(-2.6)
P 		50
C 57.9
(3)
P 		48.4
(1.5)
P 		37.1
(-0.3)
P 		20.7
(-2.9)
A 		20.3
(-3)
A 		21.5
(-2.8)
P 		38
avr 48.3
(2.3) 		52.1
(3) 		29
(-1.3) 		21.4
(-2.7) 		23.3
(-2.3) 		19.6
(-3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192880_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16754
Sequence Type Exemplar sequence
Representative Public ID C39E6.6 NCBI
Target Description C39E6.6 /REP_DB=WormBase Gene ID /WP=CE06941 /GEN=npr-1 /TR=Q18534 /GB=AAA93419.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076415(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076415 NCBI Caenorhabditis elegans Neuropeptide Receptor family member (npr-1) (npr-1) mRNA, complete cds. 11/11 None
GENEFINDER00000034202 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4767232:4769953:-1 11/11 None
C39E6.6 ENSEMBL cdna:known chromosome:CEL140:X:4767146:4769953:-1 gene:C39E6.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034200 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4767232-4769943(-) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Gene Symbol npr-1
Ensembl C39E6.6
Entrez Gene 180752 Entrez gene
SwissProt Q18534 EMBL-EBI
WormBase CE06941 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7155.1.S1_AT similar to Orexigenic neuropeptide QRFP receptor (G-protein coupled receptor 103) (SP9155) (AQ27) cfa
CHICKEN:GGA.12883.1.S1_AT similar to Orexigenic neuropeptide QRFP receptor (G protein-coupled receptor GPR103) (SP9155) (AQ27) gga
U133_X3P:HS2AFFX.1.354.S1_3P_AT G protein-coupled receptor 103 hs
HG-U133_PLUS_2:1555533_AT G protein-coupled receptor 103 hs
HG-U95D:67682_AT G protein-coupled receptor 103 hs
MOE430B:1457048_AT G protein-coupled receptor 103 mm
MOUSE430_2:1457048_AT G protein-coupled receptor 103 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
4983 neuropeptide Y receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA93419 Neuropeptide receptor family protein 1 [Caenorhabditis elegans] ref|NP_508816.1| Neuropeptide Receptor family member (npr-1) [Caenorhabditis elegans] 0.0
blast CAE68647 Hypothetical protein CBG14540 [Caenorhabditis briggsae] 0.0
blast CAD59155 Hypothetical protein T05A1.1a [Caenorhabditis elegans] emb|CAD59147.1| Hypothetical protein T05A1.1a [Caenorhabditis elegans] ref|NP_501701.2| Neuropeptide Receptor family member (npr-2) [Caenorhabditis elegans] 3.0E-82
blast CAD59155 Hypothetical protein T05A1.1a [Caenorhabditis elegans] emb|CAD59147.1| Hypothetical protein T05A1.1a [Caenorhabditis elegans] ref|NP_501701.2| Neuropeptide Receptor family member (npr-2) [Caenorhabditis elegans] 8.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 7.2E-57
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 7.2E-57
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508816.1 6 20-42,63-85,105-127,140-162,197-219,278-300
C39E6.6 6 20-42,63-85,105-127,140-162,197-219,278-300
GENEFINDER00000034202 6 25-47,67-89,109-131,144-166,201-223,282-304
Sequence
Target Sequence 
>C. ELEGANS:192880_AT
gtcaatcctagttaccatggttgtctggtttgggataacttggctgccacataacgtcat
ttctttgattattgaatatgatgacacacaatcgtttttccgactttatggcagagatga
ttacgatatcagttatttactgaaccttttcactcacagtattgccatgtcgaacaatgt
tttaaacccggtactctatgcgtggctgaacccaagtttccgtcaactggtcataaagac
atattttggagaccggcgtaaaagtgacagaataatcaatcaaacatcagtttacaaaac
aaagatcgtgcatgatacgaagcacttgaatggaagagccaaaattggcggtggtggtag
ccacgaggcgttgaaggagagggagctgaactcgtgttcggaaaatttaagctatcatgt
gaacggtcatacgaggactcctacaccggaagttcagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAATCCTAGTTACCATGGTTGTC 606 463 840 Antisense
GGTTTGGGATAACTTGGCTGCCACA 445 505 866 Antisense
CTGCCACATAACGTCATTTCTTTGA 140 237 883 Antisense
GACACACAATCGTTTTTCCGACTTT 68 367 922 Antisense
GAACCTTTTCACTCACAGTATTGCC 610 345 981 Antisense
TTAAACCCGGTACTCTATGCGTGGC 371 681 1021 Antisense
TATGCGTGGCTGAACCCAAGTTTCC 319 655 1036 Antisense
ACCCAAGTTTCCGTCAACTGGTCAT 461 89 1049 Antisense
GGAGAGGGAGCTGAACTCGTGTTCG 629 509 1215 Antisense
GAACGGTCATACGAGGACTCCTACA 40 349 1260 Antisense
GGACTCCTACACCGGAAGTTCAGTT 108 523 1274 Antisense
Sequence Source Affymetrix Proprietary Database