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[192879_s_at]

Description ZK899.8E /REP_DB=WormBase Gene ID /WP=CE23474 /GEN=gap-2 /TR=Q9TVE1 /GB=CAA86037.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=GTPase-activating protein
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 15      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 40      6grp_total > 05      4grp_total > 54      6grp_cgc > 03      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 767
1 2 3 4 range
A 1183.8
(3)
P 		857.5
(0.4)
P 		626.4
(-1.4)
P 		427.3
(-3)
P 		757
B 1005.9
(3)
P 		782.1
(0.7)
P 		651.3
(-0.6)
P 		417.2
(-3)
P 		589
C 1051.4
(3)
P 		761
(0.2)
P 		597.2
(-1.3)
P 		421.2
(-3)
P 		630
avr 1080.4
(3) 		800.2
(0.4) 		625
(-1.1) 		421.9
(-3) 		659

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 657
1 2 3 4 5 6 range
A 926.4
(2.3)
P 		987.7
(3)
P 		492.2
(-3)
P 		614.7
(-1.5)
P 		639
(-1.2)
P 		532.3
(-2.5)
P 		496
B 1069.2
(3)
P 		931.8
(1.6)
P 		475.5
(-3)
P 		480.9
(-2.9)
P 		668.8
(-1)
P 		480.5
(-2.9)
P 		594
C 1047.3
(3)
P 		869
(1.3)
P 		622.6
(-1)
P 		497.2
(-2.2)
P 		719.6
(-0.1)
P 		412.4
(-3)
P 		635
avr 1014.3
(3) 		929.5
(2.1) 		530.1
(-2.4) 		530.9
(-2.4) 		675.8
(-0.8) 		475.1
(-3) 		539
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 368
1 2 3 4 range
A 431
(3)
P 		186.1
(-1)
P 		163.8
(-1.4)
P 		62.6
(-3)
P 		368
B 320.8
(3)
P 		193.2
(-0.3)
P 		150.9
(-1.4)
P 		88.9
(-3)
P 		232
C 348
(3)
P 		218.8
(0.2)
P 		208.1
(-0)
P 		71
(-3)
P 		277
avr 366.6
(3) 		199.4
(-0.4) 		174.3
(-0.9) 		74.2
(-3) 		292

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 288
1 2 3 4 5 6 range
A 170.8
(-1.4)
P 		351.3
(3)
P 		107.2
(-3)
P 		155.9
(-1.8)
P 		192.8
(-0.9)
P 		129.6
(-2.4)
P 		244
B 372
(3)
P 		310.1
(1.6)
P 		115.5
(-3)
P 		127.4
(-2.7)
P 		271.7
(0.7)
P 		179.2
(-1.5)
P 		257
C 388.2
(3)
P 		265.1
(0.4)
P 		158.5
(-1.8)
P 		100
(-3)
P 		232.9
(-0.2)
P 		138.1
(-2.2)
P 		288
avr 310.3
(3) 		308.8
(3) 		127.1
(-3) 		127.8
(-3) 		232.5
(0.5) 		149
(-2.3) 		183
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192879_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17192
Sequence Type Exemplar sequence
Representative Public ID ZK899.8E NCBI
Target Description ZK899.8E /REP_DB=WormBase Gene ID /WP=CE23474 /GEN=gap-2 /TR=Q9TVE1 /GB=CAA86037.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=GTPase-activating protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077193(11), NM_077194(11), NM_077195(11), NM_077196(11), NM_077197(11), NM_171753(11), NM_077198(11), NM_171754(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077193 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 A 
NM_077194 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
NM_077195 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
NM_077196 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
NM_077197 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
NM_171753 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
NM_077198 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
NM_171754 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-2) (gap-2) mRNA, complete cds. 11/11 None
GENEFINDER00000005693 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9510470:9515821:1 11/11 None
SNAP00000005685 ENSEMBL cdna:SNAP chromosome:CEL140:X:9512751:9515821:1 11/11 None
ZK899.8a ENSEMBL cdna:known chromosome:CEL140:X:9472399:9516374:1 gene:ZK899.8 11/11 A 
ZK899.8b ENSEMBL cdna:known chromosome:CEL140:X:9475193:9515821:1 gene:ZK899.8 11/11 None
ZK899.8c ENSEMBL cdna:known chromosome:CEL140:X:9478939:9516372:1 gene:ZK899.8 11/11 A 
ZK899.8d ENSEMBL cdna:known chromosome:CEL140:X:9480597:9515821:1 gene:ZK899.8 11/11 None
ZK899.8e ENSEMBL cdna:known chromosome:CEL140:X:9487310:9515821:1 gene:ZK899.8 11/11 None
ZK899.8h ENSEMBL cdna:known chromosome:CEL140:X:9495225:9515821:1 gene:ZK899.8 11/11 None
ZK899.8f ENSEMBL cdna:known chromosome:CEL140:X:9501550:9515821:1 gene:ZK899.8 11/11 None
ZK899.8g ENSEMBL cdna:known chromosome:CEL140:X:9512745:9515821:1 gene:ZK899.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9487326-9515821(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK899.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86416 Hypothetical protein ZK899.8a [Caenorhabditis elegans] emb|CAA86034.1| Hypothetical protein ZK899.8a [Caenorhabditis elegans] emb|CAA85503.1| Hypothetical protein ZK899.8a [Caenorhabditis elegans] ref|NP_509594.3| GTPase Activating Protein family member (gap-2) [Caenorhabditis elegans] sp|Q8MLZ5|GAP2_CAEEL Ras GTPase-activating protein gap-2 (GTPase-activating protein 2) 0.0
blast CAA86417 Hypothetical protein ZK899.8b [Caenorhabditis elegans] emb|CAA86035.1| Hypothetical protein ZK899.8b [Caenorhabditis elegans] ref|NP_509595.1| GTPase Activating Protein family member (gap-2) [Caenorhabditis elegans] 0.0
blast CAA86419 Hypothetical protein ZK899.8e [Caenorhabditis elegans] emb|CAA86037.1| Hypothetical protein ZK899.8e [Caenorhabditis elegans] ref|NP_509598.1| GTPase Activating Protein family member (gap-2) [Caenorhabditis elegans] 0.0
blast CAA86418 Hypothetical protein ZK899.8d [Caenorhabditis elegans] emb|CAA86036.1| Hypothetical protein ZK899.8d [Caenorhabditis elegans] ref|NP_509597.1| GTPase Activating Protein family member (gap-2) [Caenorhabditis elegans] 0.0
blast CAA86415 Hypothetical protein ZK899.8c [Caenorhabditis elegans] emb|CAA86033.1| Hypothetical protein ZK899.8c [Caenorhabditis elegans] ref|NP_509596.1| GTPase Activating Protein family member (gap-2) [Caenorhabditis elegans] 0.0
blast BAA24960.1 GAP-2-4 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-7
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 6.9E-49
Sequence
Target Sequence 
>C. ELEGANS:192879_S_AT
aatccttcgagatccagctactcaaactcttcgagttcatctccagttgaacgaatggcc
gctctatcaattgctaacccagtctttggaccaggcccatcatctggatatgctatacct
gcagagccaaaggaaatcgtataccaaaagcgagcaagcccaccaccatacgatcccgat
gtgcacaactatcattatcaaccgatgcaggtctacgctgttccaccagattgtcaggtg
tccccaagaacgcaggcaacaggcggtgtcaatgctcagaatcggttaagtctgccacgg
actaatccacgagcttcgaggaattcaacacttttgcgtccgagtgtcgtaaatgttccg
gatgactgggatagaacaagtgattactggagggaccgaggcgagaacaactaccggagt
caactggaaagccaagtggaaagtcaagctcgagaaattgaacgcttgatgagagagaac
attgagctgaagagtaaaatgatgtcttcaacaaaaactgtggattccaagcgatctgac
agtggtgctagtgaggattcctacgattctttgagttcactcgatcgtccatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCCTTCGAGATCCAGCTACTCAA 340 167 2536 Antisense
AAACTCTTCGAGTTCATCTCCAGTT 625 127 2559 Antisense
TGCTAACCCAGTCTTTGGACCAGGC 526 577 2607 Antisense
GGACCAGGCCCATCATCTGGATATG 287 523 2623 Antisense
TACGATCCCGATGTGCACAACTATC 200 649 2704 Antisense
CAACCGATGCAGGTCTACGCTGTTC 30 187 2734 Antisense
TACGCTGTTCCACCAGATTGTCAGG 438 649 2749 Antisense
CAGGCGGTGTCAATGCTCAGAATCG 269 209 2795 Antisense
GGAATTCAACACTTTTGCGTCCGAG 222 533 2855 Antisense
GGATTCCTACGATTCTTTGAGTTCA 178 509 3090 Antisense
TTTGAGTTCACTCGATCGTCCATCA 297 667 3105 Antisense
Sequence Source Affymetrix Proprietary Database