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[192857_s_at] probable G protein coupled receptor

Description ZC84.4 /REP_DB=WormBase Gene ID /WP=CE24711 /TR=SW:Q03613 /GB=CAA79566.3 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=probable G protein coupled receptor
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 54      4grp_high_8_8 > 18      6grp_highest_nonorm > 60      6grp_total > 15      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 443
1 2 3 4 range
A 626.2
(3)
P 		348.8
(-1.2)
P 		233.4
(-3)
P 		627.3
(3)
P 		394
B 661.2
(3)
P 		347.7
(-1.4)
P 		232
(-3)
P 		552.8
(1.5)
P 		429
C 668.1
(3)
P 		330.5
(-1.6)
P 		225.3
(-3)
P 		526.4
(1.1)
P 		443
avr 651.8
(3) 		342.3
(-1.4) 		230.2
(-3) 		568.8
(1.8) 		422

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 487
1 2 3 4 5 6 range
A 702.5
(3)
P 		505.8
(0.3)
P 		372.9
(-1.6)
P 		317.5
(-2.3)
P 		341.7
(-2)
P 		269.9
(-3)
P 		433
B 670.4
(3)
P 		510.8
(0.5)
P 		339.7
(-2.2)
P 		399.8
(-1.2)
P 		287.9
(-3)
P 		292.6
(-2.9)
P 		383
C 500.3
(2.8)
P 		509.9
(3)
P 		311.2
(-1.1)
P 		426.4
(1.3)
P 		215.6
(-3)
P 		301.8
(-1.2)
P 		294
avr 624.4
(3) 		508.8
(1) 		341.3
(-2) 		381.2
(-1.3) 		281.7
(-3) 		288.1
(-2.9) 		343
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 228
(3)
P 		75.7
(-2.5)
P 		61
(-3)
P 		91.8
(-1.9)
P 		167
B 210.9
(3)
P 		85.9
(-1.8)
P 		53.8
(-3)
P 		117.8
(-0.6)
P 		157
C 221.2
(3)
P 		95
(-2.3)
P 		78.5
(-3)
P 		88.8
(-2.6)
P 		143
avr 220
(3) 		85.5
(-2.2) 		64.4
(-3) 		99.5
(-1.6) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 129.5
(0.4)
P 		179.9
(3)
P 		81.2
(-2.2)
P 		80.5
(-2.2)
P 		103.1
(-1)
P 		65.7
(-3)
P 		114
B 233.3
(3)
P 		170
(0.5)
P 		82.5
(-3)
P 		105.9
(-2.1)
P 		117
(-1.6)
P 		109.1
(-1.9)
P 		151
C 185.4
(3)
P 		155.6
(1.5)
P 		79.2
(-2.5)
P 		85.7
(-2.2)
P 		69.8
(-3)
P 		101
(-1.4)
P 		116
avr 182.7
(3) 		168.5
(2.2) 		81
(-3) 		90.7
(-2.4) 		96.6
(-2.1) 		91.9
(-2.4) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192857_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7229
Sequence Type Exemplar sequence
Representative Public ID ZC84.4 NCBI
Target Description ZC84.4 /REP_DB=WormBase Gene ID /WP=CE24711 /TR=SW:Q03613 /GB=CAA79566.3 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=probable G protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066637(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066637 NCBI Caenorhabditis elegans ZC84.4 (ZC84.4) mRNA, complete cds. 11/11 None
GENEFINDER00000020259 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9208229:9210346:-1 11/11 None
ZC84.4 ENSEMBL cdna:known chromosome:CEL140:III:9208209:9215344:-1 gene:ZC84.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020253 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9208224-9215335(-) 100.0 100.0
Public Domain and Genome References
Gene Title probable G protein coupled receptor
Ensembl ZC84.4
Entrez Gene 176300 Entrez gene
SwissProt Q03613 EMBL-EBI
WormBase CE24711 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA79566 Hypothetical protein ZC84.4 [Caenorhabditis elegans] ref|NP_499038.1| ZC84.4 [Caenorhabditis elegans] sp|Q03613|YN84_CAEEL Probable G-protein coupled receptor ZC84.4 0.0
blast CAE65266 Hypothetical protein CBG10160 [Caenorhabditis briggsae] 0.0
blast CAE69520 Hypothetical protein CBG15729 [Caenorhabditis briggsae] 6.0E-37
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499038.1 7 38-60,67-89,115-134,147-169,203-225,253-272,287-309
ZC84.4 7 38-60,67-89,115-134,147-169,203-225,253-272,287-309
GENEFINDER00000020259 7 34-56,63-85,111-130,143-165,199-221,249-268,283-305
Sequence
Target Sequence 
>C. ELEGANS:192857_S_AT
caacttgatcgcttgcatcacttcctatgcagtccctcttttcggaattatttactggta
tgtctcggtaccctttttcctgaaacggcgggcgctgacgacactggtcgcctcaagttc
aatggatgcagctcttcgaaaagtgatcacaactgttctccttctcacagtcatttatgt
tctctgttggacaccttactgggtcagtatgtttgctaatcgcatctggattatggagaa
aaagagcatcatcatcatttcctatttcatccatcttcttccatacatcagttgtgtcgc
ttatccattgatctttacattgctcaatcgagggattcgatcggctcacgcgaagattgt
agctgatcagagaagacgcttcagaagtcttactgatgaagcaagctcgcaaattcggac
ggcaataagaaccatccctggcacgaaaatgaagaaaaacgaatttttaactcgtacaga
agagatatcttccgacaagattgcttcagaggccgtatcagagttccgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACTTGATCGCTTGCATCACTTCC 164 189 624 Antisense
TGCAGTCCCTCTTTTCGGAATTATT 645 577 651 Antisense
GGTCGCCTCAAGTTCAATGGATGCA 103 503 729 Antisense
AAAGTGATCACAACTGTTCTCCTTC 486 121 763 Antisense
TTCTCCTTCTCACAGTCATTTATGT 319 693 779 Antisense
TATGTTCTCTGTTGGACACCTTACT 287 657 799 Antisense
TCCATCTTCTTCCATACATCAGTTG 152 591 893 Antisense
AGTTGTGTCGCTTATCCATTGATCT 314 65 913 Antisense
GCAAGCTCGCAAATTCGGACGGCAA 178 321 1024 Antisense
GGCAATAAGAACCATCCCTGGCACG 545 533 1044 Antisense
TCAGAGGCCGTATCAGAGTTCCGAG 705 609 1129 Antisense
Sequence Source Affymetrix Proprietary Database