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[192844_at] N-acetyllactosamine synthase

Description R31.3 /REP_DB=WormBase Gene ID /WP=CE27774 /GEN=osm-6 /TR=Q9TVX8 /GB=CAB61026.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=N-acetyllactosamine synthase
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 26      4grp_highest_8_8 > 54      4grp_high_8_8 > 18      6grp_highest_nonorm > 90      6grp_total > 15      4grp_total > 22      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 228.2
(2.2)
P 		172.4
(-0.5)
P 		120.1
(-3)
P 		243.7
(3)
P 		124
B 257.5
(3)
P 		100.1
(-3)
P 		108.8
(-2.7)
P 		192
(0.5)
P 		157
C 209.5
(2.4)
P 		121.2
(-1.3)
P 		79.9
(-3)
P 		223.1
(3)
P 		143
avr 231.7
(3) 		131.2
(-1.7) 		102.9
(-3) 		219.6
(2.4) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 179.8
(3)
P 		156.6
(1.5)
P 		127
(-0.5)
P 		89.9
(-3)
P 		131.3
(-0.2)
P 		99.1
(-2.4)
P 		90
B 138.8
(2.2)
P 		144.4
(3)
P 		122.9
(-0.1)
P 		108.2
(-2.2)
P 		102.5
(-3)
P 		117.3
(-0.9)
P 		42
C 177.9
(3)
P 		177.5
(3)
P 		130
(-0.6)
P 		131.8
(-0.4)
P 		97.3
(-3)
P 		119.7
(-1.3)
P 		81
avr 165.5
(3) 		159.5
(2.4) 		126.6
(-1.2) 		110
(-3) 		110.4
(-3) 		112
(-2.8) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 83.1
(3)
P 		37.4
(-2.3)
P 		31.4
(-3)
P 		35.7
(-2.5)
P 		52
B 82.1
(3)
P 		24.7
(-3)
P 		25.2
(-2.9)
P 		40.9
(-1.3)
P 		57
C 69.4
(3)
P 		34.8
(-2)
P 		27.9
(-3)
P 		37.6
(-1.6)
P 		42
avr 78.2
(3) 		32.3
(-2.5) 		28.2
(-3) 		38.1
(-1.8) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 33.2
(-1.1)
P 		55.7
(3)
P 		27.7
(-2.1)
P 		22.8
(-3)
P 		39.6
(0.1)
P 		24.1
(-2.8)
P 		33
B 48.3
(3)
P 		48.1
(2.9)
P 		29.9
(-2.6)
P 		28.6
(-3)
P 		41.6
(1)
P 		43.7
(1.6)
P 		20
C 66
(3)
P 		54.1
(1.2)
P 		33.1
(-2)
P 		26.5
(-3)
P 		31.5
(-2.2)
P 		40.1
(-0.9)
P 		40
avr 49.2
(2.2) 		52.6
(3) 		30.2
(-2.1) 		26
(-3) 		37.6
(-0.4) 		36
(-0.7) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192844_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14013
Sequence Type Exemplar sequence
Representative Public ID R31.3 NCBI
Target Description R31.3 /REP_DB=WormBase Gene ID /WP=CE27774 /GEN=osm-6 /TR=Q9TVX8 /GB=CAB61026.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=N-acetyllactosamine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171545(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171545 NCBI Caenorhabditis elegans OSMotic avoidance abnormal family member (osm-6) (osm-6) mRNA, complete cds. 11/11 None
SNAP00000005378 ENSEMBL cdna:SNAP chromosome:CEL140:V:11927258:11929418:-1 11/11 None
GENEFINDER00000005379 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11927258:11929418:-1 11/11 None
R31.3 ENSEMBL cdna:known chromosome:CEL140:V:11927209:11929994:-1 gene:R31.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11927257-11929994(-) 100.0 100.0
Public Domain and Genome References
Gene Title N-acetyllactosamine synthase
Gene Symbol osm-6
Ensembl R31.3
Entrez Gene 179631 Entrez gene
SwissProt Q9TVX8 EMBL-EBI
WormBase CE27774 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17576.1.S1_S_AT similar to NGD5 protein homolog cfa
CANINE_2:CFA.6194.1.A1_A_AT similar to NGD5 protein homolog cfa
CANINE_2:CFA.6194.1.A1_S_AT similar to NGD5 protein homolog cfa
DROSOPHILA_2:1640025_AT dm
DROSGENOME1:145907_AT dm
CHICKEN:GGA.5715.1.S1_S_AT similar to hypothetical protein MGC47065 gga
CHICKEN:GGAAFFX.11838.1.S1_AT similar to hypothetical protein MGC47065 gga
HG-U133_PLUS_2:233532_X_AT chromosome 20 open reading frame 9 hs
HG-U133B:233532_X_AT chromosome 20 open reading frame 9 hs
HU35KSUBA:RC_AA025911_AT chromosome 20 open reading frame 9 hs
HG-U133A:218709_S_AT chromosome 20 open reading frame 9 hs
HG-U133_PLUS_2:218709_S_AT chromosome 20 open reading frame 9 hs
HG-U133A_2:218709_S_AT chromosome 20 open reading frame 9 hs
U133_X3P:G7705768_3P_A_AT chromosome 20 open reading frame 9 hs
HG-U95C:65606_AT chromosome 20 open reading frame 9 hs
HG-U95D:85628_R_AT Chromosome 20 open reading frame 9 hs
U133_X3P:HS.306952.0.S1_3P_AT Chromosome 20 open reading frame 9 hs
MG-U74BV2:111332_AT cDNA sequence BC037708 mm
MOE430B:1434696_AT cDNA sequence BC037708 mm
MOUSE430_2:1434696_AT cDNA sequence BC037708 mm
MU19KSUBB:TC30997_AT CDNA sequence BC037708, mRNA (cDNA clone MGC:47065 IMAGE:4222723) mm
RAE230A:1372035_AT Similar to NGD5 protein homolog (CGI-53) (predicted) rn
RAT230_2:1372035_AT Similar to NGD5 protein homolog (CGI-53) (predicted) rn
RAE230B:1380761_AT Similar to NGD5 protein homolog (CGI-53) (predicted) rn
RAT230_2:1380761_AT Similar to NGD5 protein homolog (CGI-53) (predicted) rn
RG-U34C:RC_AI235421_AT Similar to NGD5 protein homolog (CGI-53) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB61026 Hypothetical protein R31.3 [Caenorhabditis elegans] emb|CAB61020.2| Hypothetical protein R31.3 [Caenorhabditis elegans] ref|NP_741633.1| OSMotic avoidance abnormal family member (osm-6) [Caenorhabditis elegans] emb|CAA03975.1| osm-6 [Caenorhabditis elegans] 0.0
blast CAE75349 Hypothetical protein CBG23329 [Caenorhabditis briggsae] 0.0
blast T24240 hypothetical protein R31.3 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:192844_AT
tgaacacgatagatgctgcagagccggaaattaatgattacacaaatattccagatcata
ttcatatgtcccagcagatcaagtttacaataagttttagtgtaaaacatcatttctact
tgaataaacatttttattctagattgtatgaagcattgaatctttcacctccaccactta
cccttgttgaacctcaatttgaacttccaatgcctccatttcaacctgcagtatttccac
caacatttcaagaattaccaatgcctcctcttgaattatttgatcttgatgaacagttca
gctctccggaaattcaactttctcagctcgcaaatagaagcgaggaagaagatctcatat
tctttattgaaaaagctggtgaaattactggaatatctgctgaattgacaagaagtgaac
gaacccccaagaaaataattgagttggcagtgagcaaactcatgttattcaaacgttcaa
tgatggatggagagttggaagttgcatcagcgttcgatatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACACGATAGATGCTGCAGAGCC 577 573 767 Antisense
CATATTCATATGTCCCAGCAGATCA 562 213 823 Antisense
CACTTACCCTTGTTGAACCTCAATT 306 193 941 Antisense
AATTTGAACTTCCAATGCCTCCATT 204 175 962 Antisense
CCTCCATTTCAACCTGCAGTATTTC 360 267 979 Antisense
TGCAGTATTTCCACCAACATTTCAA 5 579 993 Antisense
ACCAATGCCTCCTCTTGAATTATTT 83 83 1023 Antisense
AACAGTTCAGCTCTCCGGAAATTCA 151 123 1058 Antisense
TCAACTTTCTCAGCTCGCAAATAGA 295 631 1080 Antisense
GACAAGAAGTGAACGAACCCCCAAG 386 335 1173 Antisense
GGAAGTTGCATCAGCGTTCGATATT 482 531 1263 Antisense
Sequence Source Affymetrix Proprietary Database