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[192834_at] Eukaryotic protein kinase domain [2]

Description Y81G3A.3 /REP_DB=WormBase Gene ID /WP=CE23118 /TR=Q9XVY4 /GB=CAA22515.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain (2)
Cluster 6grp_total > 37      4grp_total > 51      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 87.5
(-0.2)
A 		123
(3)
A 		55.8
(-3)
A 		76.4
(-1.2)
A 		67
B 32.9
(-3)
A 		64.7
(3)
A 		64.4
(2.9)
A 		49.4
(0.1)
A 		32
C 72.7
(2.3)
A 		61.1
(1.2)
A 		80.5
(3)
A 		14.3
(-3)
A 		66
avr 64.4
(-0.1) 		82.9
(3) 		66.9
(0.3) 		46.7
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 78.3
(0.5)
A 		63.5
(-0.7)
A 		53.7
(-1.5)
A 		35.8
(-3)
A 		50.4
(-1.8)
A 		108.9
(3)
P 		73
B 51.9
(1.2)
A 		65
(3)
A 		20.3
(-3)
A 		50.7
(1.1)
A 		42.8
(0)
A 		53
(1.4)
A 		45
C 127.2
(3)
P 		64.3
(-1.1)
A 		35.9
(-3)
A 		106.8
(1.7)
A 		55.3
(-1.7)
A 		37.3
(-2.9)
A 		91
avr 85.8
(3) 		64.3
(0.4) 		36.6
(-3) 		64.4
(0.4) 		49.5
(-1.4) 		66.4
(0.6) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 31.8
(3)
A 		26.7
(1.5)
A 		14.6
(-2)
A 		11.2
(-3)
A 		21
B 10.5
(-3)
A 		16
(3)
A 		14.9
(1.8)
A 		10.5
(-3)
A 		6
C 24.1
(2.1)
A 		17.6
(0.6)
A 		28
(3)
A 		2.4
(-3)
A 		26
avr 22.1
(3) 		20.1
(2.1) 		19.2
(1.8) 		8
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 14.4
(-1.2)
A 		22.6
(1.7)
A 		11.7
(-2.1)
A 		9.1
(-3)
A 		15.2
(-0.9)
A 		26.5
(3)
P 		17
B 18.1
(1.7)
A 		21.6
(3)
A 		4.9
(-3)
A 		13.4
(0.1)
A 		17.4
(1.5)
A 		19.8
(2.4)
A 		17
C 47.1
(3)
P 		19.6
(-1.3)
A 		9.1
(-3)
A 		21.5
(-1)
A 		17.9
(-1.6)
A 		12.5
(-2.5)
A 		38
avr 26.5
(3) 		21.3
(1.3) 		8.6
(-3) 		14.7
(-1) 		16.8
(-0.3) 		19.6
(0.7) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192834_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3317
Sequence Type Exemplar sequence
Representative Public ID Y81G3A.3 NCBI
Target Description Y81G3A.3 /REP_DB=WormBase Gene ID /WP=CE23118 /TR=Q9XVY4 /GB=CAA22515.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain (2)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064380(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064380 NCBI Caenorhabditis elegans Y81G3A.3 (Y81G3A.3) mRNA, complete cds. 11/11 None
SNAP00000006873 ENSEMBL cdna:SNAP chromosome:CEL140:II:13084065:13090957:1 11/11 None
GENEFINDER00000006878 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13084065:13090957:1 11/11 None
Y81G3A.3 ENSEMBL cdna:known chromosome:CEL140:II:13084065:13091382:1 gene:Y81G3A.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13084061-13090954(+) 97.82 100.0
Public Domain and Genome References
Gene Title Eukaryotic protein kinase domain [2]
Ensembl Y81G3A.3
Entrez Gene 174953 Entrez gene
SwissProt Q9XVY4 EMBL-EBI
WormBase CE23118 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251520_AT protein kinase family protein at
CANINE:1591208_S_AT similar to Eukaryotic translation initiation factor 2-alpha kinase 4 (GCN2-like protein) cfa
CANINE_2:CFA.19193.1.S1_AT similar to Eukaryotic translation initiation factor 2-alpha kinase 4 (GCN2-like protein) cfa
CANINE_2:CFA.19193.1.S1_S_AT similar to Eukaryotic translation initiation factor 2-alpha kinase 4 (GCN2-like protein) cfa
DROSOPHILA_2:1632728_AT dm
DROSGENOME1:154131_AT dm
CHICKEN:GGA.17364.1.S1_S_AT similar to GCN2 eIF2alpha kinase gga
CHICKEN:GGAAFFX.22981.1.S1_AT similar to GCN2 eIF2alpha kinase gga
MOUSE430A_2:1421389_A_AT eukaryotic translation initiation factor 2 alpha kinase 4 mm
MOUSE430_2:1421389_A_AT eukaryotic translation initiation factor 2 alpha kinase 4 mm
MOE430A:1421389_A_AT eukaryotic translation initiation factor 2 alpha kinase 4 mm
MU11KSUBB:MSA.20151.0_S_AT eukaryotic translation initiation factor 2 alpha kinase 4 mm
MG-U74AV2:94941_AT eukaryotic translation initiation factor 2 alpha kinase 4 mm
MU19KSUBC:TC36303_AT Eukaryotic translation initiation factor 2 alpha kinase 4, mRNA (cDNA clone IMAGE:5321284) mm
RG-U34B:RC_AA924361_AT eukaryotic translation initiation factor 2 alpha kinase 4 (predicted) rn
RAE230A:1374224_AT eukaryotic translation initiation factor 2 alpha kinase 4 (predicted) rn
RAT230_2:1374224_AT eukaryotic translation initiation factor 2 alpha kinase 4 (predicted) rn
YEAST_2:1774468_AT Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA22515 Hypothetical protein Y81G3A.3 [Caenorhabditis elegans] ref|NP_496781.1| Y81G3A.3 [Caenorhabditis elegans] 0.0
blast CAE59423 Hypothetical protein CBG02792 [Caenorhabditis briggsae] 0.0
blast CAG10683 unnamed protein product [Tetraodon nigroviridis] 3.0E-68
Protein Families
Method ID Description E-Value
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 8.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-13
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-35
Pfam IPR006575 EMBL-EBI RWD 7.2E-37
Sequence
Target Sequence 
>C. ELEGANS:192834_AT
ggacgtggactcggcgatttcaagtgtgtggcgaggagtccaagctcttgatggtcaaag
tattcatatgactccatgtggtggtggaccgatctcttcaataagcactcctggagatgc
tcatcatcatgatgatcatcacccgggaactcctgttattgcgtccaaaagttcggtatc
aacaacagtggcgacgacgagcatccgaccgatcagtgcgacggtggcgaatctcaatgt
gatccttgtcacgtcggccgacagattccacaaagttatgaaggagaagaaacgagtgga
gagtcaagtcaggaaccatctcacagaatttgtggctcacttcacgagcaagaccaggat
cgaggtgttggtgtgtgatattccggctgatgtgatcaagaagatcgttagcgagctcac
gaagactagttcagaagctgagatcgacaagctattcgatcagctgatccagaaacatgg
aaaagtcgacctgtcaccgctccgaagacaatttcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACGTGGACTCGGCGATTTCAAGT 472 521 4497 Antisense
TGTGGCGAGGAGTCCAAGCTCTTGA 96 553 4523 Antisense
GACCGATCTCTTCAATAAGCACTCC 274 383 4583 Antisense
GCACTCCTGGAGATGCTCATCATCA 229 315 4601 Antisense
GGAACTCCTGTTATTGCGTCCAAAA 103 525 4642 Antisense
ATCAGTGCGACGGTGGCGAATCTCA 139 27 4708 Antisense
TTGTCACGTCGGCCGACAGATTCCA 640 701 4742 Antisense
GTCAAGTCAGGAACCATCTCACAGA 709 463 4799 Antisense
GTTGGTGTGTGATATTCCGGCTGAT 386 437 4863 Antisense
ATGGAAAAGTCGACCTGTCACCGCT 274 53 4973 Antisense
GTCACCGCTCCGAAGACAATTTCAC 613 465 4989 Antisense
Sequence Source Affymetrix Proprietary Database