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[192832_s_at] proteolipid protein PPA1 like protein

Description T01H3.1 /REP_DB=WormBase Gene ID /WP=CE03595 /TR=Q22087 /GB=CAA92686.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=proteolipid protein PPA1 like protein
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 33      4grp_high_8_8 > 59      6grp_highest_nonorm > 04      6grp_total > 23      4grp_total > 60      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3529
1 2 3 4 range
A 6687
(2.2)
P 		5634.1
(-0)
P 		7043.9
(3)
P 		4251.7
(-3)
P 		2792
B 7268
(3)
P 		6722.1
(2.1)
P 		6804.6
(2.2)
P 		3738.9
(-3)
P 		3529
C 6193
(2.4)
P 		6427
(3)
P 		6009.3
(2)
P 		3976.9
(-3)
P 		2450
avr 6716
(3) 		6261.1
(2) 		6619.3
(2.8) 		3989.2
(-3) 		2727

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2945
1 2 3 4 5 6 range
A 5187
(1.9)
P 		5645.2
(3)
P 		4816.8
(1.1)
P 		4914.1
(1.3)
P 		3278.1
(-2.4)
P 		3037.7
(-3)
P 		2608
B 5738.8
(3)
P 		5424.4
(2.3)
P 		3981.9
(-0.7)
P 		3637.5
(-1.5)
P 		3989.8
(-0.7)
P 		2927.4
(-3)
P 		2811
C 5380.6
(2.7)
P 		5503.7
(3)
P 		5378.6
(2.7)
P 		3725.1
(-0.9)
P 		3667.9
(-1.1)
P 		2794.3
(-3)
P 		2709
avr 5435.5
(2.8) 		5524.4
(3) 		4725.8
(1.2) 		4092.2
(-0.3) 		3645.3
(-1.3) 		2919.8
(-3) 		2605
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1812
1 2 3 4 range
A 2434.7
(3)
P 		1223
(-1)
P 		1841.4
(1)
P 		622.4
(-3)
P 		1812
B 2317.7
(3)
P 		1660.2
(0.4)
P 		1576.8
(0.1)
P 		796.8
(-3)
P 		1521
C 2050
(2.8)
P 		1847.8
(2)
P 		2094.4
(3)
P 		670.6
(-3)
P 		1424
avr 2267.5
(3) 		1577
(0.4) 		1837.5
(1.4) 		696.6
(-3) 		1571

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1268
1 2 3 4 5 6 range
A 956.2
(-2)
P 		2007.7
(3)
P 		1049
(-1.5)
P 		1246.3
(-0.6)
P 		989.2
(-1.8)
P 		739.6
(-3)
P 		1268
B 1996.8
(3)
P 		1805.6
(1.9)
P 		966.9
(-3)
P 		963.5
(-3)
P 		1621
(0.8)
P 		1091.6
(-2.3)
P 		1033
C 1994.4
(3)
P 		1679.3
(1.5)
P 		1368.9
(-0)
P 		749.1
(-3)
P 		1186.9
(-0.9)
P 		935.4
(-2.1)
P 		1245
avr 1649.1
(1.8) 		1830.9
(3) 		1128.3
(-1.6) 		986.3
(-2.6) 		1265.7
(-0.7) 		922.2
(-3) 		909
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192832_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4188
Sequence Type Exemplar sequence
Representative Public ID T01H3.1 NCBI
Target Description T01H3.1 /REP_DB=WormBase Gene ID /WP=CE03595 /TR=Q22087 /GB=CAA92686.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=proteolipid protein PPA1 like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063258(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063258 NCBI Caenorhabditis elegans T01H3.1 (T01H3.1) mRNA, complete cds. 11/11 None
SNAP00000018637 ENSEMBL cdna:SNAP chromosome:CEL140:II:7878334:7879234:-1 11/11 None
GENEFINDER00000018643 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7878334:7879234:-1 11/11 None
T01H3.1.2 ENSEMBL cdna:known chromosome:CEL140:II:7878027:7879237:-1 gene:T01H3.1 11/11 None
T01H3.1.1 ENSEMBL cdna:known chromosome:CEL140:II:7878027:7879257:-1 gene:T01H3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7878330-7879231(-) 100.0 100.0
Public Domain and Genome References
Gene Title proteolipid protein PPA1 like protein
Ensembl T01H3.1
Entrez Gene 174272 Entrez gene
SwissProt Q22087 EMBL-EBI
WormBase CE03595 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:19133_AT vacuolar ATP synthase, putative / V-ATPase, putative at
ATH1-121501:253442_AT vacuolar ATP synthase, putative / V-ATPase, putative at
CANINE_2:CFA.21006.1.S1_A_AT similar to ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c cfa
CANINE_2:CFA.21006.2.S1_S_AT similar to ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c cfa
DROSOPHILA_2:1637024_AT dm
DROSGENOME1:142158_AT dm
CHICKEN:GGA.4592.1.S1_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' gga
PLASMODIUM_ANOPHELES:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HG-U133B:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HG-U133A:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HG-U133_PLUS_2:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HG-U133A_2:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HG-FOCUS:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
U133_X3P:200078_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HUGENEFL:D89052_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
U133_X3P:G13543437_3P_S_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
HG-U95AV2:36167_AT ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'' hs
MU11KSUBA:AA396633_S_AT ATPase, H+ transporting, V0 subunit B mm
MG-U74AV2:98104_AT ATPase, H+ transporting, V0 subunit B mm
MOE430A:1425448_X_AT ATPase, H+ transporting, V0 subunit B mm
MOUSE430A_2:1425448_X_AT ATPase, H+ transporting, V0 subunit B mm
MOUSE430_2:1425448_X_AT ATPase, H+ transporting, V0 subunit B mm
MOE430A:1437013_X_AT ATPase, H+ transporting, V0 subunit B mm
MOUSE430A_2:1437013_X_AT ATPase, H+ transporting, V0 subunit B mm
MOUSE430_2:1437013_X_AT ATPase, H+ transporting, V0 subunit B mm
MOE430A:1416769_S_AT ATPase, H+ transporting, V0 subunit B mm
MOUSE430A_2:1416769_S_AT ATPase, H+ transporting, V0 subunit B mm
MOUSE430_2:1416769_S_AT ATPase, H+ transporting, V0 subunit B mm
MU19KSUBC:TC40957_S_AT ATPase, H+ transporting, V0 subunit B, mRNA (cDNA clone MGC:40642 IMAGE:4922226) mm
RAT230_2:1398930_AT ATPase, H+ transporting, V0 subunit B (predicted) rn
RAE230A:1398930_AT ATPase, H+ transporting, V0 subunit B (predicted) rn
RG-U34C:RC_AI177593_AT ATPase, H+ transporting, V0 subunit B (predicted) rn
RAE230B:1398554_AT ATPase, H+ transporting, V0 subunit B (predicted) rn
RAT230_2:1398554_AT ATPase, H+ transporting, V0 subunit B (predicted) rn
RG-U34B:RC_AI030761_AT ATPase, H+ transporting, V0 subunit B (predicted) rn
YEAST_2:1779820_AT Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
15986 ATP synthesis coupled proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16469 proton-transporting two-sector ATPase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
46933 hydrogen-transporting ATP synthase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
46961 hydrogen-transporting ATPase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92686 Hypothetical protein T01H3.1 [Caenorhabditis elegans] ref|NP_495659.1| T01H3.1 [Caenorhabditis elegans] dbj|BAA22597.1| VHA-4 [Caenorhabditis elegans] 2.0E-89
blast CAE59826 Hypothetical protein CBG03298 [Caenorhabditis briggsae] 3.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR002379 EMBL-EBI H+-transporting two-sector ATPase, C subunit 1.7E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495659.1 5 13-35,50-72,93-115,144-166,179-201
T01H3.1.2 5 13-35,50-72,93-115,144-166,179-201
T01H3.1.1 5 13-35,50-72,93-115,144-166,179-201
SNAP00000018637 5 13-35,50-72,93-115,144-166,179-201
GENEFINDER00000018643 5 13-35,50-72,93-115,144-166,179-201
Sequence
Target Sequence 
>C. ELEGANS:192832_S_AT
tcacaaccctgatcattcttggaacagggttgttttacatgctcagtggacaaggacatc
gatttgacattggatggttcttgacatcgacatcaccacatatgtgggctggtcttggta
tcggattctcgctttcgctttccgtcttgggagccggatggggaatcttcacaacgggat
cttcaattctcggaggaggagttaaggcaccaagaattagaacaaagaatcttgtttcta
ttattttctgcgaagctgttgctattttcggtattatcatggctttcgtgttcgtcggaa
agctcgctgaattccgtcgtgaagatcttcctgatactgaagacggaatggcaatccttg
ctcgtaacctcgcttcaggatacatgatctttggaggtggtctcaccgtcggactctcca
accttgtctgtggtcttgctgtcggaatcgtcggatctggagctgccattgctgatgccg
ccaatccagctcttttcgtcaagattctcattattgaaattttcgcttccgctattggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACAACCCTGATCATTCTTGGAAC 382 627 59 Antisense
GGGGAATCTTCACAACGGGATCTTC 187 497 218 Antisense
TATTATCATGGCTTTCGTGTTCGTC 471 661 330 Antisense
GAATTCCGTCGTGAAGATCTTCCTG 143 325 367 Antisense
GAATGGCAATCCTTGCTCGTAACCT 407 331 404 Antisense
CTCGTAACCTCGCTTCAGGATACAT 359 233 419 Antisense
TTTGGAGGTGGTCTCACCGTCGGAC 702 661 448 Antisense
GTCTTGCTGTCGGAATCGTCGGATC 18 469 491 Antisense
AATCGTCGGATCTGGAGCTGCCATT 256 169 504 Antisense
CCAATCCAGCTCTTTTCGTCAAGAT 526 277 539 Antisense
GAAATTTTCGCTTCCGCTATTGGAC 557 363 574 Antisense
Sequence Source Affymetrix Proprietary Database