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[192831_s_at] galactoside binding lectin

Description C44F1.3 /REP_DB=WormBase Gene ID /WP=CE02163 /GEN=lec-4 /TR=Q18625 /GB=CAA88850.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=galactoside binding lectin
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 62      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 25      6grp_total > 62      4grp_total > 60      6grp_highest_sub > 03      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3562
1 2 3 4 range
A 3531.2
(1.8)
P
4231.4
(3)
P
3898.4
(2.4)
P
669.9
(-3)
P
3562
B 3801.3
(2.3)
P
4197.5
(3)
P
4109
(2.8)
P
774.7
(-3)
P
3423
C 3754.6
(2.3)
P
4165.8
(3)
P
3904.9
(2.5)
P
831.6
(-3)
P
3334
avr 3695.7
(2.1)
4198.2
(3)
3970.8
(2.6)
758.7
(-3)
3440

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3586
1 2 3 4 5 6 range
A 3208.5
(-3)
P
4422.5
(-1)
P
6794.4
(3)
P
5752
(1.3)
P
3956.4
(-1.7)
P
3281.6
(-2.9)
P
3586
B 3706.7
(-2.5)
P
4335.2
(-1.3)
P
6706.9
(3)
P
5925
(1.6)
P
4656.3
(-0.7)
P
3419.7
(-3)
P
3287
C 3599
(-2.7)
P
4165.8
(-1.6)
P
6377.2
(3)
P
5860.2
(1.9)
P
4711.7
(-0.4)
P
3467.1
(-3)
P
2910
avr 3504.7
(-2.8)
4307.8
(-1.3)
6626.2
(3)
5845.7
(1.6)
4441.5
(-1)
3389.5
(-3)
3237
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1263
1 2 3 4 range
A 1285.7
(3)
P
918.5
(1.1)
P
1019.1
(1.7)
P
98.1
(-3)
P
1188
B 1212.2
(3)
P
1036.7
(2)
P
952.1
(1.5)
P
165.1
(-3)
P
1047
C 1242.9
(2.4)
P
1197.7
(2.2)
P
1361
(3)
P
140.2
(-3)
P
1221
avr 1246.9
(3)
1051
(1.9)
1110.7
(2.3)
134.5
(-3)
1112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1300
1 2 3 4 5 6 range
A 591.5
(-3)
P
1572.9
(3)
P
1479.7
(2.4)
P
1458.8
(2.3)
P
1193.9
(0.7)
P
799
(-1.7)
P
981
B 1289.7
(-2.9)
P
1443
(-1.4)
P
1628.6
(0.4)
P
1569.3
(-0.1)
P
1891.8
(3)
P
1275.2
(-3)
P
617
C 1334
(-0.8)
P
1271.1
(-1.6)
P
1623.1
(3)
P
1178.5
(-2.8)
P
1524.6
(1.7)
P
1160.6
(-3)
P
463
avr 1071.7
(-3)
1429
(1.2)
1577.1
(3)
1402.2
(0.9)
1536.8
(2.5)
1078.3
(-2.9)
505
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192831_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6506
Sequence Type Exemplar sequence
Representative Public ID C44F1.3 NCBI
Target Description C44F1.3 /REP_DB=WormBase Gene ID /WP=CE02163 /GEN=lec-4 /TR=Q18625 /GB=CAA88850.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=galactoside binding lectin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065362(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_065362 NCBI Caenorhabditis elegans gaLECtin family member (lec-4) (lec-4) mRNA, complete cds. 11/11 None
SNAP00000024032 ENSEMBL cdna:SNAP chromosome:CEL140:III:3771500:3773200:-1 11/11 None
GENEFINDER00000024038 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3771500:3774886:-1 11/11 None
C44F1.3 ENSEMBL cdna:known chromosome:CEL140:III:3771453:3773282:-1 gene:C44F1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3771499-3773037(-) 99.77 99.77
Public Domain and Genome References
Gene Title galactoside binding lectin
Gene Symbol lec-4
Ensembl C44F1.3
Entrez Gene 175488 Entrez gene
SwissProt Q18625 EMBL-EBI
WormBase CE02163 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5529 sugar binding inferred from electronic annotation QuickGO AmiGO
16936 galactoside binding inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88850 Hypothetical protein C44F1.3 [Caenorhabditis elegans] ref|NP_497763.1| gaLECtin family member (lec-4) [Caenorhabditis elegans] 1.0E-166
blast BAB11970.1 galectin LEC-4 [Caenorhabditis elegans] 1.0E-165
blast CAA88850 Hypothetical protein C44F1.3 [Caenorhabditis elegans] ref|NP_497763.1| gaLECtin family member (lec-4) [Caenorhabditis elegans] 1.0E-165
blast BAB11970.1 galectin LEC-4 [Caenorhabditis elegans] 1.0E-164
blast CAE64495 Hypothetical protein CBG09221 [Caenorhabditis briggsae] 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.7E-26
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 3.0E-19
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.7E-26
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 3.0E-19
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.7E-26
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 3.0E-19
Sequence
Target Sequence
>C. ELEGANS:192831_S_AT
gaattcgatcttcgcatccgagttctcgacgaaggccttgaaatctcggctgacaacaag
aagatccacgagttcaaacatcgtcttccattccaatccattgaatatctttctgttcgt
ggtgacctttccttgaatggaattcattggggtggaagattctataaacttccatgggag
accgcattcccagctggatttcttgaaaagggacaacgtgttcatttgtatggtattcca
aagggtgatcgttggtcacttgatttggtcgctcgtaatcaggatattttgttccatttc
aatccaagaatcaaggataaggcggtcgtccgtaactctcaccgtaatggattctgggac
accgaggaacgtgaaggtggattcccattcaagaaggacgtcggattcgatttgaccatt
gtcaacgaagaatattccattcaaatcttcatcaatcgcgaacgcttcggaactttccag
caccgtacccagaatccaattggt


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GAATTCGATCTTCGCATCCGAGTTC 326 323 298 Antisense
CGAAGGCCTTGAAATCTCGGCTGAC 611 253 327 Antisense
CGAGTTCAAACATCGTCTTCCATTC 185 249 366 Antisense
ATATCTTTCTGTTCGTGGTGACCTT 73 21 402 Antisense
AAACTTCCATGGGAGACCGCATTCC 658 127 463 Antisense
CGCATTCCCAGCTGGATTTCTTGAA 387 255 480 Antisense
CGTTGGTCACTTGATTTGGTCGCTC 439 247 547 Antisense
GGATAAGGCGGTCGTCCGTAACTCT 18 511 612 Antisense
AACTCTCACCGTAATGGATTCTGGG 703 139 631 Antisense
ATCGCGAACGCTTCGGAACTTTCCA 104 35 752 Antisense
GCACCGTACCCAGAATCCAATTGGT 98 313 777 Antisense
Sequence Source Affymetrix Proprietary Database