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[192822_s_at]

Description T13A10.11 /REP_DB=WormBase Gene ID /WP=CE07492 /TR=SW:Q27522 /GB=AAB38126.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=S-adenosylmethionine synthetase
Cluster 6grp_total > 98      4grp_total > 09      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 3.6
(-3)
A 		5.1
(-2.1)
A 		5.3
(-2)
A 		14.1
(3)
A 		11
B 2.6
(-3)
A 		4.4
(-1.4)
A 		6.1
(0.1)
A 		9.4
(3)
A 		7
C 2.8
(-3)
A 		6.5
(-2.4)
A 		2.5
(-3)
A 		43.3
(3)
A 		41
avr 3
(-3) 		5.3
(-2.3) 		4.6
(-2.5) 		22.3
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 553
1 2 3 4 5 6 range
A 6.4
(-3)
A 		2.6
(-3)
A 		5.4
(-3)
A 		117.1
(-1.7)
P 		407.3
(1.6)
P 		533.4
(3)
P 		531
B 2.8
(-3)
A 		3.9
(-3)
A 		4.9
(-3)
A 		154.5
(-1.4)
P 		492.2
(2.3)
P 		555.5
(3)
P 		553
C 3.5
(-3)
A 		3.9
(-3)
A 		3.1
(-3)
A 		139.1
(-1.4)
P 		404.2
(1.8)
P 		508.8
(3)
P 		506
avr 4.2
(-3) 		3.5
(-3) 		4.5
(-3) 		136.9
(-1.5) 		434.6
(1.9) 		532.6
(3) 		529
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.3
(-1.8)
A 		1.1
(-3)
A 		1.4
(-1.2)
A 		2.1
(3)
A 		1
B 0.8
(-3)
A 		1.1
(-1.5)
A 		1.4
(-0)
A 		2
(3)
A 		1
C 0.9
(-3)
A 		1.9
(-2.1)
A 		0.9
(-3)
A 		7.3
(3)
A 		6
avr 1
(-3) 		1.4
(-2.1) 		1.2
(-2.6) 		3.8
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 1.2
(-3)
A 		0.9
(-3)
A 		1.2
(-3)
A 		29.7
(-1.7)
P 		122.9
(2.7)
P 		129.9
(3)
P 		129
B 1
(-3)
A 		1.3
(-3)
A 		1.2
(-3)
A 		40.9
(-1.8)
P 		200
(2.8)
P 		207.2
(3)
P 		206
C 1.3
(-3)
A 		1.2
(-3)
A 		0.8
(-3)
A 		28
(-2)
P 		130.8
(1.6)
P 		170.3
(3)
P 		170
avr 1.2
(-3) 		1.1
(-3) 		1.1
(-3) 		32.9
(-1.9) 		151.2
(2.4) 		169.1
(3) 		168
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192822_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10430
Sequence Type Exemplar sequence
Representative Public ID T13A10.11 NCBI
Target Description T13A10.11 /REP_DB=WormBase Gene ID /WP=CE07492 /TR=SW:Q27522 /GB=AAB38126.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=S-adenosylmethionine synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171354(11), NM_171355(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171354 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-32) (tag-32) mRNA, complete cds. 11/11 None
NM_171355 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-32) (tag-32) mRNA, complete cds. 11/11 None
SNAP00000039096 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6277465:6279126:-1 11/11 None
GENEFINDER00000039103 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6277465:6284351:-1 11/11 None
T13A10.11a.1 ENSEMBL cdna:known chromosome:CEL140:IV:6277441:6279497:-1 gene:T13A10.11 11/11 None
T13A10.11a.2 ENSEMBL cdna:known chromosome:CEL140:IV:6277441:6279168:-1 gene:T13A10.11 11/11 None
T13A10.11b ENSEMBL cdna:known chromosome:CEL140:IV:6277465:6279515:-1 gene:T13A10.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6277470-6279132(-) 100.0 100.0
Public Domain and Genome References
Ensembl T13A10.11
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741415 Temporarily Assigned Gene name family member (tag-32) [Caenorhabditis elegans] sp|Q27522|METN_CAEEL Probable S-adenosylmethionine synthetase T13A10.11 (Methionine adenosyltransferase) (AdoMet synthetase) gb|AAB38126.2| Temporarily assigned gene name protein 32, isoform a [Caenorhabditis elegans] 0.0
blast T16856 hypothetical protein T13A10.11 - Caenorhabditis elegans 0.0
blast AAA82279 Hypothetical protein C06E7.3a [Caenorhabditis elegans] ref|NP_500871.1| C06E7.3a [Caenorhabditis elegans] sp|P50306|METL_CAEEL Probable S-adenosylmethionine synthetase C06E7.3 (Methionine adenosyltransferase) (AdoMet synthetase) 0.0
blast NP_741416 Temporarily Assigned Gene name family member (tag-32) [Caenorhabditis elegans] gb|AAM97949.1| Temporarily assigned gene name protein 32, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.3E-66
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.1E-97
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 6.2E-91
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.8E-27
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.1E-97
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 6.2E-91
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.3E-52
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.5E-7
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.1E-97
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 6.2E-91
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.3E-52
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.5E-7
Pfam IPR005040 EMBL-EBI Caenorhabditis protein of unknown function DUF280 1.0E-126
Pfam IPR005040 EMBL-EBI Caenorhabditis protein of unknown function DUF280 1.0E-126
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.1E-97
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 6.2E-91
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000039103 12 15-37,50-72,101-123,136-158,187-209,230-247,262-284,468-490,513-535,599-621,636-658,671-693
Sequence
Target Sequence 
>C. ELEGANS:192822_S_AT
ttatcgacaacgacaccgtctaccatctcaatccatgcggatcatttgtcgttggtggac
caatgggtgatgctggacttactggacgtaagatcatcgtagacacctacggaggatggg
gagctcacggaggaggtgctttctctggaaaggacccaaccaaagtcgatcgttctgccg
cttatgctgctcgctgggttgccaagtcacttgtcaaggctggtctctgccgtcgttgtc
tcgttcaagtttcctatgctatcggagttgctaagccgctttcggtgatggtcttctcgt
ttggaacttctgcactcaacgaggctgaactcttgaagattgtcaacgacaactttgact
tgagaccaggaatgatcatcaaaaatctggacttgaagagagccatctatgagccaaccg
ctgaaaatggtcacttcggtcacaacgattttccatgggagcaggctcgtaagctcaaga
ttgataaagatctccgcgccaaatccaaaggacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATCGACAACGACACCGTCTACCA 287 669 725 Antisense
CATGCGGATCATTTGTCGTTGGTGG 306 209 758 Antisense
TCACTTGTCAAGGCTGGTCTCTGCC 554 625 931 Antisense
TGTCTCGTTCAAGTTTCCTATGCTA 125 559 961 Antisense
AAGCCGCTTTCGGTGATGGTCTTCT 263 149 997 Antisense
CTCGTTTGGAACTTCTGCACTCAAC 263 231 1020 Antisense
TGCACTCAACGAGGCTGAACTCTTG 588 577 1035 Antisense
AAGAGAGCCATCTATGAGCCAACCG 582 153 1120 Antisense
GAAAATGGTCACTTCGGTCACAACG 59 355 1147 Antisense
GGTCACAACGATTTTCCATGGGAGC 71 503 1162 Antisense
TCTCCGCGCCAAATCCAAAGGACCA 161 611 1215 Antisense
Sequence Source Affymetrix Proprietary Database