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[192821_at]

Description C14A11.3 /REP_DB=WormBase Gene ID /WP=CE08151 /TR=O02134 /GB=AAB52870.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanine-nucleotide releasing factor
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 70      6grp_total > 04      4grp_total > 54      6grp_cgc > 41      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 361.2
(3)
P 		208.4
(-1.1)
P 		177.2
(-2)
P 		138.2
(-3)
P 		223
B 324.6
(3)
P 		249.6
(0.4)
P 		203.4
(-1.2)
P 		149.5
(-3)
P 		175
C 285.4
(3)
P 		190
(-1)
P 		206.1
(-0.4)
P 		144
(-3)
P 		141
avr 323.7
(3) 		216
(-0.6) 		195.6
(-1.3) 		143.9
(-3) 		180

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 295
1 2 3 4 5 6 range
A 299
(3)
P 		248.4
(0.6)
P 		181.4
(-2.5)
P 		229.5
(-0.3)
P 		269.8
(1.6)
P 		171.7
(-3)
P 		127
B 321.8
(3)
P 		300.5
(2)
P 		218.6
(-1.9)
P 		302.4
(2.1)
P 		196.2
(-3)
P 		198.1
(-2.9)
P 		126
C 467.1
(3)
P 		314.6
(-0.4)
P 		283.5
(-1.1)
P 		269.2
(-1.4)
P 		276.2
(-1.2)
P 		195.7
(-3)
P 		271
avr 362.6
(3) 		287.8
(0.4) 		227.8
(-1.6) 		267
(-0.3) 		247.4
(-1) 		188.5
(-3) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 131.5
(3)
P 		45.2
(-1.7)
P 		46.3
(-1.6)
P 		20.2
(-3)
P 		111
B 103.5
(3)
P 		61.6
(-0.5)
P 		47.1
(-1.7)
P 		31.9
(-3)
P 		72
C 94.5
(3)
P 		54.6
(-0.4)
P 		71.8
(1.1)
P 		24.3
(-3)
P 		70
avr 109.8
(3) 		53.8
(-1) 		55.1
(-0.9) 		25.5
(-3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 55.1
(-1.1)
P 		88.3
(3)
P 		39.5
(-3)
P 		58.2
(-0.7)
P 		81.4
(2.2)
P 		41.8
(-2.7)
P 		49
B 112
(3)
P 		100
(1.8)
P 		53.1
(-3)
P 		80.1
(-0.2)
P 		79.7
(-0.3)
P 		73.9
(-0.9)
P 		59
C 173.1
(3)
P 		96
(-0.9)
P 		72.2
(-2.1)
P 		54.1
(-3)
P 		89.4
(-1.2)
P 		65.5
(-2.4)
P 		119
avr 113.4
(3) 		94.8
(1.1) 		54.9
(-3) 		64.1
(-2.1) 		83.5
(-0.1) 		60.4
(-2.4) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192821_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18505
Sequence Type Exemplar sequence
Representative Public ID C14A11.3 NCBI
Target Description C14A11.3 /REP_DB=WormBase Gene ID /WP=CE08151 /TR=O02134 /GB=AAB52870.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanine-nucleotide releasing factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171641(11), NM_171642(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171641 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-150) (tag-150) mRNA, complete cds. 11/11 None
NM_171642 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-150) (tag-150) mRNA, complete cds. 11/11 None
GENEFINDER00000039990 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2795707:2798763:1 11/11 None
SNAP00000039980 ENSEMBL cdna:SNAP chromosome:CEL140:X:2796056:2798763:1 11/11 None
C14A11.3b ENSEMBL cdna:known chromosome:CEL140:X:2785594:2799224:1 gene:C14A11.3 11/11 None
C14A11.3a ENSEMBL cdna:known chromosome:CEL140:X:2793929:2798763:1 gene:C14A11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2794004-2798763(+) 100.0 100.0
Public Domain and Genome References
Ensembl C14A11.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52871 Temporarily assigned gene name protein 150, isoform b [Caenorhabditis elegans] ref|NP_741736.1| Temporarily Assigned Gene name family member (tag-150) [Caenorhabditis elegans] 0.0
blast AAB52870 Temporarily assigned gene name protein 150, isoform a [Caenorhabditis elegans] ref|NP_741737.1| Temporarily Assigned Gene name family member (tag-150) [Caenorhabditis elegans] 0.0
blast CAE58834 Hypothetical protein CBG02046 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 5.1E-8
Pfam IPR000219 EMBL-EBI DH domain 1.6E-15
Pfam IPR001849 EMBL-EBI Pleckstrin-like 5.1E-8
Pfam IPR000219 EMBL-EBI DH domain 1.6E-15
Pfam IPR001849 EMBL-EBI Pleckstrin-like 5.1E-8
Pfam IPR000219 EMBL-EBI DH domain 1.6E-15
Pfam IPR001849 EMBL-EBI Pleckstrin-like 5.1E-8
Pfam IPR000219 EMBL-EBI DH domain 1.6E-15
Sequence
Target Sequence 
>C. ELEGANS:192821_AT
agatctgaaatcgctcggactgcttcgtcttcaaacggaatgcgatgtgttcacctacaa
taggaagaagaaggccaagctgtcacgtgctcagaaaagattcatcttcttttttgatgg
agccgttatgttctgcaagaaacgcgtcagcaatccaggaactactctcaactcggagcc
tgaatactttgagcacaagttctgtattccgatcatctctctgggctacgatacatcttc
tcgcactggcgcctcacgatttgaagtttgggacgacgcgaaaaccgacgcctacgtcat
tgaaactattgatcaaacggctcgcaccaaatggattcagcgtctcggaaaaagtgagac
tgcacaagacgcttgcctggaaaacaggcaaaggccaaagagctgggcgagcactgtgtc
gaacgagagttcgtgctcgtcgagcacccgtgaatcggacagcactgatagcacaatgga
caccaatgggaatacacagacgacgcaagtggacgttccttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATCTGAAATCGCTCGGACTGCTT 437 67 777 Antisense
GGACTGCTTCGTCTTCAAACGGAAT 613 523 793 Antisense
AAGAAGGCCAAGCTGTCACGTGCTC 178 153 844 Antisense
ACTACTCTCAACTCGGAGCCTGAAT 634 99 937 Antisense
AAGTTCTGTATTCCGATCATCTCTC 44 157 973 Antisense
CTGGGCTACGATACATCTTCTCGCA 710 239 997 Antisense
ACTGGCGCCTCACGATTTGAAGTTT 106 97 1021 Antisense
CAAACGGCTCGCACCAAATGGATTC 596 189 1090 Antisense
CTGGGCGAGCACTGTGTCGAACGAG 614 239 1179 Antisense
GTCGAGCACCCGTGAATCGGACAGC 104 475 1215 Antisense
GACGACGCAAGTGGACGTTCCTTAC 515 379 1275 Antisense
Sequence Source Affymetrix Proprietary Database