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[192820_at] glutathione S-transferase

Description R03D7.6 /REP_DB=WormBase Gene ID /WP=CE01613 /GEN=gst-5 /TR=SW:Q09596 /GB=CAA86859.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione transferase
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 06      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 02      6grp_total > 07      4grp_total > 39      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1735
1 2 3 4 range
A 2517.5
(3)
P 		801.8
(-3)
P 		1004.2
(-2.3)
P 		1571.6
(-0.3)
P 		1716
B 2401.5
(3)
P 		997.6
(-3)
P 		1046.6
(-2.8)
P 		1464.4
(-1)
P 		1404
C 2536.8
(3)
P 		1249.3
(-1.8)
P 		917.2
(-3)
P 		1403.5
(-1.2)
P 		1620
avr 2485.3
(3) 		1016.2
(-2.9) 		989.3
(-3) 		1479.8
(-1) 		1496

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4650
1 2 3 4 5 6 range
A 5085.5
(3)
P 		2241.1
(-0.7)
P 		2473.4
(-0.4)
P 		1604
(-1.5)
P 		923.8
(-2.4)
P 		436
(-3)
P 		4650
B 4883.6
(3)
P 		2139.2
(-0.8)
P 		2621.4
(-0.1)
P 		1408.7
(-1.8)
P 		923.8
(-2.5)
P 		550.3
(-3)
P 		4333
C 4472.3
(3)
P 		2106.6
(-0.7)
P 		2526.1
(-0)
P 		1627.5
(-1.4)
P 		812
(-2.7)
P 		614.7
(-3)
P 		3858
avr 4813.8
(3) 		2162.3
(-0.7) 		2540.3
(-0.2) 		1546.7
(-1.6) 		886.5
(-2.5) 		533.7
(-3) 		4280
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 743
1 2 3 4 range
A 916.6
(3)
P 		174.1
(-3)
P 		262.5
(-2.3)
P 		230.1
(-2.5)
P 		743
B 765.8
(3)
P 		246.4
(-3)
P 		242.5
(-3)
P 		312.1
(-2.2)
P 		523
C 839.7
(3)
P 		359.2
(-1.8)
P 		319.7
(-2.2)
P 		236.6
(-3)
P 		603
avr 840.7
(3) 		259.9
(-3) 		274.9
(-2.8) 		259.6
(-3) 		581

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1593
1 2 3 4 5 6 range
A 937.5
(3)
P 		797
(2)
P 		538.7
(0.1)
P 		406.8
(-0.8)
P 		278.8
(-1.8)
P 		106.1
(-3)
P 		831
B 1699.3
(3)
P 		712.1
(-1)
P 		636.6
(-1.3)
P 		373.1
(-2.3)
P 		375.3
(-2.3)
P 		205.2
(-3)
P 		1494
C 1657.7
(3)
P 		642.8
(-1.2)
P 		642.9
(-1.2)
P 		327.3
(-2.5)
P 		262.7
(-2.8)
P 		205.8
(-3)
P 		1452
avr 1431.5
(3) 		717.3
(-0.4) 		606.1
(-0.9) 		369.1
(-2.1) 		305.6
(-2.4) 		172.4
(-3) 		1259
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192820_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3167
Sequence Type Exemplar sequence
Representative Public ID R03D7.6 NCBI
Target Description R03D7.6 /REP_DB=WormBase Gene ID /WP=CE01613 /GEN=gst-5 /TR=SW:Q09596 /GB=CAA86859.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063956(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063956 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-5) (gst-5) mRNA, complete cds. 11/11 None
SNAP00000040571 ENSEMBL cdna:SNAP chromosome:CEL140:II:10950324:10951474:1 11/11 None
GENEFINDER00000040580 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10950324:10951474:1 11/11 None
R03D7.6 ENSEMBL cdna:known chromosome:CEL140:II:10950324:10951514:1 gene:R03D7.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10950320-10951471(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-5
Ensembl R03D7.6
Entrez Gene 187537 Entrez gene
SwissProt Q09596 EMBL-EBI
WormBase CE01613 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86859 Hypothetical protein R03D7.6 [Caenorhabditis elegans] ref|NP_496357.1| Glutathione S-Transferase family member (gst-5) [Caenorhabditis elegans] sp|Q09596|GST5_CAEEL Probable glutathione S-transferase 5 (GST class-sigma) pdb|1ZL9|B Chain B, Crystal Structure Of A Major Nematode C.Elegans Specific Gst (Ce01613) pdb|1ZL9|A Chain A, Crystal Structure Of A Major Nematode C.Elegans Specific Gst (Ce01613) 1.0E-117
blast CAE73226 Hypothetical protein CBG20632 [Caenorhabditis briggsae] 1.0E-94
blast CAE58992 Hypothetical protein CBG02265 [Caenorhabditis briggsae] emb|CAE56959.1| Hypothetical protein CBG24809 [Caenorhabditis briggsae] 2.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 3.1E-6
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 6.1E-19
Sequence
Target Sequence 
>C. ELEGANS:192820_AT
gacctacttcaatggacgtggcgctggagaagtgtctcgtcagattttcgcctatgccgg
acaacaatacgaggataatagagtcactcaggaacaatggccagcattgaaagaaacctg
cgctgctccattcggacaacttccattcctcgaagtcgacggtaagaagcttgctcaatc
ccacgcgattgctcgtttcttggctcgtgagttcaagctcaacggaaaaaccgcctggga
agaggctcaagtgaactctcttgccgatcaatacaaggattattcaagtgaggctcgtcc
atatttctacgctgtcatgggattcggtccaggagacgttgaaactttgaagaaagacat
cttccttccagcatttgaaaagttctacggattcttggtcaacttcttgaaggcttcggg
atccggattccttgtcggagactctttgacctggattgacttggctattgcccaacattc
agctgatttgattgccaagggaggtgatttcagcaagttcccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCTACTTCAATGGACGTGGCGCT 541 381 30 Antisense
AGAAGTGTCTCGTCAGATTTTCGCC 486 75 57 Antisense
TTGAAAGAAACCTGCGCTGCTCCAT 363 705 136 Antisense
TTCGGACAACTTCCATTCCTCGAAG 418 695 160 Antisense
GAGGCTCAAGTGAACTCTCTTGCCG 219 405 271 Antisense
AAGTGAGGCTCGTCCATATTTCTAC 603 157 315 Antisense
TCTACGCTGTCATGGGATTCGGTCC 643 611 335 Antisense
TCGGGATCCGGATTCCTTGTCGGAG 296 605 445 Antisense
GAGACTCTTTGACCTGGATTGACTT 496 391 467 Antisense
GGCTATTGCCCAACATTCAGCTGAT 155 539 492 Antisense
GAGGTGATTTCAGCAAGTTCCCAGA 230 403 530 Antisense
Sequence Source Affymetrix Proprietary Database