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[192799_s_at]

Description F49E11.1B /REP_DB=WormBase Gene ID /WP=CE19878 /TR=Q9XTF3 /GB=CAB03351.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase (2 domains)
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 29      4grp_highest_8_8 > 0      4grp_high_8_8 > 37      6grp_highest_nonorm > 82      6grp_total > 38      4grp_total > 43      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 233
1 2 3 4 range
A 418.1
(-2.2)
P 		549.3
(3)
P 		397.8
(-3)
P 		469.1
(-0.2)
P 		152
B 419.2
(-2.1)
P 		412.4
(-3)
P 		459.9
(3)
P 		456.1
(2.5)
P 		48
C 372.4
(-1.2)
P 		506.9
(3)
P 		385.9
(-0.8)
P 		316.3
(-3)
P 		191
avr 403.2
(-3) 		489.5
(3) 		414.5
(-2.2) 		413.8
(-2.3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 478
1 2 3 4 5 6 range
A 373.3
(0.2)
P 		493.2
(2.1)
P 		174.4
(-3)
P 		357.7
(-0.1)
P 		327.1
(-0.6)
P 		549.2
(3)
P 		375
B 356.4
(1.3)
P 		385.3
(3)
P 		292.9
(-2.4)
P 		283
(-3)
P 		317.8
(-1)
P 		294.4
(-2.3)
P 		102
C 651.9
(3)
P 		365.1
(-1.3)
P 		355.9
(-1.4)
P 		247.1
(-3)
P 		396
(-0.8)
P 		350.2
(-1.5)
P 		405
avr 460.5
(3) 		414.5
(1.5) 		274.4
(-3) 		295.9
(-2.3) 		347
(-0.7) 		397.9
(1) 		186
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 152.2
(3)
P 		119.2
(0.6)
P 		104
(-0.5)
P 		68.7
(-3)
P 		84
B 133.7
(3)
P 		101.9
(-2.2)
P 		106.6
(-1.5)
P 		97.2
(-3)
P 		37
C 123.3
(1.5)
P 		145.7
(3)
P 		134.5
(2.3)
P 		53.3
(-3)
P 		92
avr 136.4
(3) 		122.3
(1.7) 		115
(1) 		73.1
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 68.8
(-1.7)
P 		175.4
(3)
P 		38
(-3)
P 		90.7
(-0.7)
P 		98.7
(-0.3)
P 		133.7
(1.2)
P 		137
B 124
(2.5)
P 		128.3
(2.9)
P 		71.1
(-3)
P 		75
(-2.6)
P 		129.1
(3)
P 		109.8
(1)
P 		58
C 241.6
(3)
P 		111.4
(-1.1)
P 		90.6
(-1.7)
P 		49.7
(-3)
P 		128.1
(-0.5)
P 		117.2
(-0.9)
P 		192
avr 144.8
(3) 		138.4
(2.5) 		66.6
(-3) 		71.8
(-2.6) 		118.6
(1) 		120.2
(1.1) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192799_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8548
Sequence Type Exemplar sequence
Representative Public ID F49E11.1B NCBI
Target Description F49E11.1B /REP_DB=WormBase Gene ID /WP=CE19878 /TR=Q9XTF3 /GB=CAB03351.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028037(11), NM_001028036(11), NM_070091(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028037 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 None
NM_001028036 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 None
NM_070091 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 A 
SNAP00000011564 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13031304:13033921:1 11/11 None
GENEFINDER00000011587 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13031449:13034986:1 11/11 None
F49E11.1c ENSEMBL cdna:known chromosome:CEL140:IV:13009105:13035916:1 gene:F49E11.1 11/11 A 
F49E11.1b ENSEMBL cdna:known chromosome:CEL140:IV:13009122:13035916:1 gene:F49E11.1 11/11 A 
F49E11.1a ENSEMBL cdna:known chromosome:CEL140:IV:13031206:13034085:1 gene:F49E11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13009133-13034494(+) 100.0 100.0
Public Domain and Genome References
Ensembl F49E11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03528 Hypothetical protein F49E11.1b [Caenorhabditis elegans] emb|CAB03351.1| Hypothetical protein F49E11.1b [Caenorhabditis elegans] emb|CAA94353.1| Hypothetical protein F49E11.1b [Caenorhabditis elegans] ref|NP_001023207.1| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] 0.0
blast CAB54274 Hypothetical protein F49E11.1c [Caenorhabditis elegans] emb|CAB54308.2| Hypothetical protein F49E11.1c [Caenorhabditis elegans] emb|CAB54254.2| Hypothetical protein F49E11.1c [Caenorhabditis elegans] ref|NP_001023208.1| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] gb|AAM09088.1| minibrain kinase 2 [Caenorhabditis elegans] 0.0
blast CAA94352 Hypothetical protein F49E11.1a [Caenorhabditis elegans] ref|NP_502492.2| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] 0.0
blast CAE56332 Hypothetical protein CBG23998 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-61
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-61
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-63
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-63
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 5.0E-63
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 3.0E-63
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 2.0E-66
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 9.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 9.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 9.2E-68
Sequence
Target Sequence 
>C. ELEGANS:192799_S_AT
gttcatcttgcttcgacgatcaacgtatatacacatatattcagtccagattctacagag
ctccagaagtgattcttgggacaaaatacggaatgccaatagacatgtggtcacttggat
gtattcttgccgagcttctcaccggatatccattattgcctggagaggatgagaatgacc
agctggcacttatcattgaactactcggaatgccaccaccgaaaagtttagaaacagcga
aacgtgcgcgtactttcatcacatcgaaaggatatccacgttattgcacggccacatcga
tgccagatggttcggtagttctcgccggagcacgatcgaagcgaggaaagatgcgtggac
caccggcttcgcggtcgtggtcgacagcgttgaagaacatgggtgacgagttattcgtgg
atttcttgaagagatgtctcgattgggatccggagaccagaatgactccagctcaagctt
tgaaacacaagtggcttcgtcgcaggttacccaatccaccacgggatggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCATCTTGCTTCGACGATCAACG 537 443 1838 Antisense
CAGTCCAGATTCTACAGAGCTCCAG 33 205 1879 Antisense
AGCTTCTCACCGGATATCCATTATT 307 79 1970 Antisense
GACCAGCTGGCACTTATCATTGAAC 678 383 2014 Antisense
TTGAACTACTCGGAATGCCACCACC 646 703 2033 Antisense
GATATCCACGTTATTGCACGGCCAC 437 425 2108 Antisense
CACGGCCACATCGATGCCAGATGGT 90 199 2124 Antisense
TTCTCGCCGGAGCACGATCGAAGCG 247 689 2156 Antisense
GAAAGATGCGTGGACCACCGGCTTC 46 361 2183 Antisense
AGACCAGAATGACTCCAGCTCAAGC 228 71 2291 Antisense
TTACCCAATCCACCACGGGATGGAC 254 679 2344 Antisense
Sequence Source Affymetrix Proprietary Database