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[192795_at] 3-oxo-5-alpha-steroid 4-dehydrogenase

Description F19H6.4 /REP_DB=WormBase Gene ID /WP=CE03235 /TR=Q19605 /GB=CAA92168.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=3-oxo-5-alpha-steroid 4-dehydrogenase
Cluster 6grp_total > 89      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 66
(3)
A 		20.9
(-3)
A 		60
(2.2)
A 		65.4
(2.9)
A 		45
B 68.7
(1.5)
A 		55.1
(-3)
A 		69.1
(1.7)
A 		73.1
(3)
A 		18
C 27.1
(-0.2)
A 		5.6
(-3)
A 		51.5
(3)
A 		21.7
(-0.9)
A 		46
avr 53.9
(1.9) 		27.2
(-3) 		60.2
(3) 		53.4
(1.8) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 108
(-0.2)
A 		47.3
(-3)
A 		71.5
(-1.9)
A 		70.5
(-1.9)
A 		136.8
(1.1)
P 		178
(3)
P 		131
B 49.5
(-1.3)
A 		77.2
(-0.2)
A 		8.6
(-3)
A 		61.3
(-0.8)
A 		155.4
(3)
P 		150.7
(2.8)
P 		147
C 87.3
(-2.3)
A 		76.9
(-2.7)
A 		90.7
(-2.1)
A 		71
(-3)
A 		170.4
(1.4)
P 		206.5
(3)
P 		136
avr 81.6
(-1.8) 		67.1
(-2.5) 		56.9
(-3) 		67.6
(-2.5) 		154.2
(1.8) 		178.4
(3) 		122
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 24
(3)
A 		4.5
(-3)
A 		15.7
(0.4)
A 		9.6
(-1.4)
A 		20
B 21.9
(3)
A 		13.6
(-3)
A 		16
(-1.3)
A 		15.6
(-1.6)
A 		8
C 9
(-0.3)
A 		1.6
(-3)
A 		18
(3)
A 		3.7
(-2.2)
A 		16
avr 18.3
(3) 		6.6
(-3) 		16.6
(2.1) 		9.6
(-1.5) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 19.9
(-2.1)
A 		16.8
(-2.7)
A 		15.6
(-3)
A 		17.9
(-2.5)
A 		41.3
(2.6)
P 		43.3
(3)
P 		28
B 17.2
(-1.5)
A 		25.7
(-0.7)
A 		2.1
(-3)
A 		16.2
(-1.6)
A 		63.1
(3)
P 		56.2
(2.3)
P 		61
C 32.4
(-1)
A 		23.5
(-2)
A 		23.1
(-2)
A 		14.3
(-3)
A 		55.1
(1.5)
P 		69.1
(3)
P 		55
avr 23.2
(-1.6) 		22
(-1.8) 		13.6
(-3) 		16.1
(-2.6) 		53.2
(2.6) 		56.2
(3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192795_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16160
Sequence Type Exemplar sequence
Representative Public ID F19H6.4 NCBI
Target Description F19H6.4 /REP_DB=WormBase Gene ID /WP=CE03235 /TR=Q19605 /GB=CAA92168.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=3-oxo-5-alpha-steroid 4-dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077676(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077676 NCBI Caenorhabditis elegans F19H6.4 (F19H6.4) mRNA, complete cds. 11/11 None
SNAP00000003013 ENSEMBL cdna:SNAP chromosome:CEL140:X:12365609:12367036:1 11/11 None
GENEFINDER00000003019 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12365630:12367036:1 11/11 None
F19H6.4 ENSEMBL cdna:known chromosome:CEL140:X:12365609:12367036:1 gene:F19H6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12365608-12367036(+) 100.0 100.0
Public Domain and Genome References
Gene Title 3-oxo-5-alpha-steroid 4-dehydrogenase
Ensembl F19H6.4
Entrez Gene 184699 Entrez gene
SwissProt Q19605 EMBL-EBI
WormBase CE03235 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582956_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) cfa
CANINE_2:CFA.3579.1.S1_S_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) cfa
CANINE:1582955_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) cfa
HG-U133_PLUS_2:206938_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
HG-U133A:206938_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
HG-FOCUS:206938_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
HG-U133A_2:206938_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
HG-U95AV2:565_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
HC-G110:565_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
HUGENEFL:M74047_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
U133_X3P:G4557854_3P_AT steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) hs
MOUSE430A_2:1422960_AT steroid 5 alpha-reductase 2 mm
MOE430A:1422960_AT steroid 5 alpha-reductase 2 mm
MOUSE430_2:1422960_AT steroid 5 alpha-reductase 2 mm
RT-U34:M95058COMPLETE_SEQ_AT steroid 5-alpha-reductase 2 rn
RG-U34A:M95058COMPLETE_SEQ_AT steroid 5-alpha-reductase 2 rn
RAE230A:1387580_AT steroid 5-alpha-reductase 2 rn
RAT230_2:1387580_AT steroid 5-alpha-reductase 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92168 Hypothetical protein F19H6.4 [Caenorhabditis elegans] ref|NP_510077.1| F19H6.4 [Caenorhabditis elegans] 1.0E-144
blast CAA92168 Hypothetical protein F19H6.4 [Caenorhabditis elegans] ref|NP_510077.1| F19H6.4 [Caenorhabditis elegans] 1.0E-141
blast CAA92173 Hypothetical protein F42F12.3 [Caenorhabditis elegans] ref|NP_510071.1| F42F12.3 [Caenorhabditis elegans] 1.0E-101
blast CAA92173 Hypothetical protein F42F12.3 [Caenorhabditis elegans] ref|NP_510071.1| F42F12.3 [Caenorhabditis elegans] 9.0E-99
blast CAE57261 Hypothetical protein CBG00143 [Caenorhabditis briggsae] 1.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR001104 EMBL-EBI 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal 2.6E-98
Pfam IPR001104 EMBL-EBI 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal 2.6E-98
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510077.1 3 4-23,99-121,191-213
F19H6.4 3 4-23,99-121,191-213
SNAP00000003013 3 4-23,99-121,191-213
GENEFINDER00000003019 3 5-27,92-114,184-206
Sequence
Target Sequence 
>C. ELEGANS:192795_AT
atcaatcccaaactcgcgtggttcattcaagaggctcccgctttcttcattcccctttac
tacctgagacgcggaccttccaattctggatatgaactgaacgctgctttcctcttccac
tactttttcagagcgttgatctatccttgccgcatccgatcgactacaaaatctccagtc
gcaattgtcgccgcagcaacattcttctgtgcctacaatggatttttgcaaggatattgg
aatgctttttatcaaccggaggaaccttggaccacgcgtaaactcgttgggtcgctcatg
tattgcactggaatgtacatcaaccacaagtcggacacgattcttcgagagctacgggct
gatggtgggactggatacaagattccaactgggttcttgtacgaatatatctcttgcccc
aattatgccggagagatcatggagtggatcggctactcaatccttgcctggaatcttcct
gcactagcattcgccatcttcacgattgccaacattggtccaagagccgttgcacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAATCCCAAACTCGCGTGGTTCA 313 25 136 Antisense
ATATGAACTGAACGCTGCTTTCCTC 61 21 225 Antisense
CTTCCACTACTTTTTCAGAGCGTTG 73 219 249 Antisense
CAGAGCGTTGATCTATCCTTGCCGC 29 207 264 Antisense
CAAAATCTCCAGTCGCAATTGTCGC 95 191 302 Antisense
GTAAACTCGTTGGGTCGCTCATGTA 655 461 413 Antisense
AGCTACGGGCTGATGGTGGGACTGG 86 65 485 Antisense
TTGTACGAATATATCTCTTGCCCCA 660 707 532 Antisense
CTTGCCCCAATTATGCCGGAGAGAT 19 219 548 Antisense
ACTCAATCCTTGCCTGGAATCTTCC 637 99 590 Antisense
CATTGGTCCAAGAGCCGTTGCACAT 239 215 648 Antisense
Sequence Source Affymetrix Proprietary Database