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[192794_at]

Description F55C5.3 /REP_DB=WormBase Gene ID /WP=CE11154 /GEN=twk-11 /TR=Q20817 /GB=CAB01568.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 42      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 90      6grp_total > 30      4grp_total > 21      6grp_highest_sub > 03      6grp_cgc > 73      6grp_high1_sub > 02_03      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 209
1 2 3 4 range
A 203.2
(3)
P 		94.8
(-1.9)
P 		69.3
(-3)
P 		198.5
(2.8)
P 		134
B 147.3
(-0.4)
P 		169.6
(0.2)
P 		57.5
(-3)
P 		266.6
(3)
P 		209
C 212.5
(2.4)
P 		153.5
(-0.9)
P 		117.8
(-3)
P 		222.1
(3)
P 		104
avr 187.7
(1.3) 		139.3
(-0.7) 		81.5
(-3) 		229.1
(3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 101.5
(-2.7)
P 		163.9
(0.5)
P 		213.3
(3)
P 		197.8
(2.2)
P 		172.6
(0.9)
P 		96.4
(-3)
P 		117
B 151.5
(-0.7)
P 		224.9
(2.7)
P 		230.8
(3)
P 		163.9
(-0.1)
P 		150.4
(-0.7)
P 		101.2
(-3)
P 		130
C 142.5
(-1.6)
P 		192.6
(2)
P 		180.2
(1.1)
P 		206.6
(3)
P 		147.3
(-1.2)
P 		122.6
(-3)
P 		84
avr 131.8
(-1.5) 		193.8
(2.2) 		208.1
(3) 		189.4
(1.9) 		156.8
(-0) 		106.7
(-3) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 74
(3)
P 		20.6
(-2.7)
P 		18.1
(-3)
P 		29
(-1.8)
P 		56
B 47
(1.6)
P 		41.9
(0.9)
P 		13.3
(-3)
P 		56.8
(3)
P 		44
C 70.4
(3)
P 		44.1
(-1.8)
P 		41
(-2.4)
P 		37.5
(-3)
P 		33
avr 63.8
(3) 		35.5
(-1.3) 		24.1
(-3) 		41.1
(-0.4) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 18.7
(-3)
P 		58.3
(3)
P 		46.5
(1.2)
P 		50.2
(1.8)
P 		52.1
(2.1)
P 		23.5
(-2.3)
P 		40
B 52.7
(-0.6)
P 		74.8
(3)
P 		56
(-0)
P 		43.4
(-2.1)
P 		61.1
(0.8)
P 		37.7
(-3)
P 		37
C 52.8
(1)
P 		58.8
(3)
P 		45.9
(-1.3)
P 		41.6
(-2.8)
P 		47.7
(-0.7)
P 		41
(-3)
P 		18
avr 41.4
(-1.5) 		64
(3) 		49.5
(0.1) 		45.1
(-0.8) 		53.6
(0.9) 		34.1
(-3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192794_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14032
Sequence Type Exemplar sequence
Representative Public ID F55C5.3 NCBI
Target Description F55C5.3 /REP_DB=WormBase Gene ID /WP=CE11154 /GEN=twk-11 /TR=Q20817 /GB=CAB01568.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028810(10), NM_001028809(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028810 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-24) (twk-24) mRNA, complete cds. 10/11 None
NM_001028809 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-24) (twk-24) mRNA, complete cds. 11/11 None
SNAP00000005344 ENSEMBL cdna:SNAP chromosome:CEL140:V:12269257:12271898:-1 9/11 None
GENEFINDER00000005363 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12268471:12272370:-1 10/11 None
F55C5.3b ENSEMBL cdna:known chromosome:CEL140:V:12268319:12271898:-1 gene:F55C5.3 10/11 None
F55C5.3a ENSEMBL cdna:known chromosome:CEL140:V:12268471:12271898:-1 gene:F55C5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000005343 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12268470-12271898(-) 97.95 100.0
Public Domain and Genome References
Ensembl F55C5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAH04700 Hypothetical protein F55C5.3b [Caenorhabditis elegans] ref|NP_001023981.1| TWiK family of potassium channels family member (twk-24) [Caenorhabditis elegans] 0.0
blast CAB01568 Hypothetical protein F55C5.3a [Caenorhabditis elegans] ref|NP_001023980.1| TWiK family of potassium channels family member (twk-24) [Caenorhabditis elegans] gb|AAC32865.1| putative potassium channel subunit n2P24 [Caenorhabditis elegans] 0.0
blast CAE75434 Hypothetical protein CBG23427 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023981.1 6 118-140,224-246,258-280,359-381,388-406,416-438
NP_001023980.1 6 118-140,224-246,258-280,359-381,388-406,416-438
F55C5.3b 6 118-140,224-246,258-280,359-381,388-406,416-438
F55C5.3a 6 118-140,224-246,258-280,359-381,388-406,416-438
SNAP00000005344 6 118-140,224-246,258-280,359-381,388-406,416-438
GENEFINDER00000005363 6 165-187,269-291,301-323,414-431,435-457,464-486
Sequence
Target Sequence 
>C. ELEGANS:192794_AT
gtaccaatcaaatcagcggcaatctttttctttctttggattatgatttccgcgtttatt
gtcagattatgggaatacgaatggacttatttcactgcattttactttttcttcacatct
ctaacaacaattggattaggagatgtggttacaaaaacaccaaattttatcatttttaat
ctggcaatgacattaattggactatcagttgttggattatgtgcagctattgtacaagca
aaagtcaaattggtattcgatcgaatgttgaggagtattgatgcacaatacagaattcgc
caagttgatcctcatgttgcaacaatgagtattgtagaagatgaagaagaaggtgtgaaa
cgattaattcaatcacaatcattagaagaccgaatcatatttttgtttgttgatgaacac
aagaaaagtatgctgaaagagcgaataactcaaacgtatccatcaaaagctgataaatat
gttcaaactggtcaacgagtatatgatcaacgtcaaggatcggacgaagatctgagtgat
gaagatgaagaagatgttgaatatgatgaacttggaaataagattattcctccacctatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACCAATCAAATCAGCGGCAATCT 643 457 1093 Antisense
CAGCGGCAATCTTTTTCTTTCTTTG 313 203 1106 Antisense
GATTATGATTTCCGCGTTTATTGTC 568 429 1131 Antisense
GAATGGACTTATTTCACTGCATTTT 231 333 1171 Antisense
ACTGCATTTTACTTTTTCTTCACAT 205 99 1186 Antisense
TTTCTTCACATCTCTAACAACAATT 141 673 1200 Antisense
GTTGTTGGATTATGTGCAGCTATTG 689 437 1300 Antisense
GAATTCGCCAAGTTGATCCTCATGT 652 323 1385 Antisense
GACCGAATCATATTTTTGTTTGTTG 674 381 1480 Antisense
GCGAATAACTCAAACGTATCCATCA 47 289 1533 Antisense
AAATAAGATTATTCCTCCACCTATT 284 117 1668 Antisense
Sequence Source Affymetrix Proprietary Database