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[192784_at] guanine nucleotide binding protein

Description C14B1.4 /REP_DB=WormBase Gene ID /WP=CE00901 /TR=SW:Q17963 /GB=CAA85487.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=guanine nucleotide binding protein
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 86      6grp_total > 59      4grp_total > 53      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 533
1 2 3 4 range
A 1191.6
(2.7)
P 		1216.9
(3)
P 		1189.7
(2.6)
P 		773.3
(-3)
P 		444
B 1277.4
(3)
P 		1108.5
(-0.2)
P 		959.7
(-3)
P 		987.5
(-2.5)
P 		318
C 1306.1
(3)
P 		1071.2
(-1)
P 		1030
(-1.7)
P 		951.7
(-3)
P 		354
avr 1258.4
(3) 		1132.2
(0.9) 		1059.8
(-0.4) 		904.2
(-3) 		354

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 579
1 2 3 4 5 6 range
A 1218.6
(1.5)
P 		1044.3
(-0.3)
P 		781.9
(-3)
P 		871.2
(-2.1)
P 		1093.5
(0.2)
P 		1360.9
(3)
P 		579
B 1119.5
(1.6)
P 		925.7
(-1.2)
P 		832
(-2.5)
P 		799.8
(-3)
P 		1155.5
(2.1)
P 		1216.7
(3)
P 		417
C 1162
(1.6)
P 		910.2
(-3)
P 		935.8
(-2.5)
P 		1024.6
(-0.9)
P 		927.8
(-2.7)
P 		1240.9
(3)
P 		331
avr 1166.7
(1.5) 		960.1
(-1.4) 		849.9
(-3) 		898.5
(-2.3) 		1058.9
(-0) 		1272.8
(3) 		423
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 321
1 2 3 4 range
A 433.9
(3)
P 		264.2
(-0.2)
P 		311
(0.7)
P 		113.2
(-3)
P 		321
B 407.4
(3)
P 		273.8
(-1.1)
P 		222.4
(-2.6)
P 		210.4
(-3)
P 		197
C 432.4
(3)
P 		308
(0.3)
P 		359
(1.4)
P 		160.5
(-3)
P 		272
avr 424.6
(3) 		282
(-0.3) 		297.5
(0.1) 		161.4
(-3) 		263

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 299
1 2 3 4 5 6 range
A 224.6
(-1.4)
P 		371.4
(3)
P 		170.3
(-3)
P 		220.9
(-1.5)
P 		330
(1.8)
P 		331.3
(1.8)
P 		201
B 389.5
(1.2)
P 		308.1
(-0.6)
P 		202
(-3)
P 		211.8
(-2.8)
P 		469.5
(3)
P 		453.7
(2.6)
P 		268
C 430.7
(3)
P 		277.7
(-1.1)
P 		238.2
(-2.1)
P 		206.1
(-3)
P 		300.2
(-0.5)
P 		415.4
(2.6)
P 		225
avr 348.3
(1.4) 		319.1
(0.5) 		203.5
(-3) 		212.9
(-2.7) 		366.6
(2) 		400.1
(3) 		197
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192784_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8095
Sequence Type Exemplar sequence
Representative Public ID C14B1.4 NCBI
Target Description C14B1.4 /REP_DB=WormBase Gene ID /WP=CE00901 /TR=SW:Q17963 /GB=CAA85487.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=guanine nucleotide binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065348(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065348 NCBI Caenorhabditis elegans C14B1.4 (C14B1.4) mRNA, complete cds. 11/11 None
SNAP00000040960 ENSEMBL cdna:SNAP chromosome:CEL140:III:3705265:3706555:1 11/11 None
GENEFINDER00000040972 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3705265:3712953:1 11/11 None
C14B1.4 ENSEMBL cdna:known chromosome:CEL140:III:3705265:3706785:1 gene:C14B1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3705264-3706555(+) 99.56 100.0
Public Domain and Genome References
Gene Title guanine nucleotide binding protein
Ensembl C14B1.4
Entrez Gene 175474 Entrez gene
SwissProt Q17963 EMBL-EBI
WormBase CE00901 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:252273_AT transducin family protein / WD-40 repeat family protein at
CANINE_2:CFAAFFX.30346.1.S1_S_AT similar to WD repeat domain 5 cfa
DROSOPHILA_2:1640099_AT will die slowly dm
DROSGENOME1:144410_AT will die slowly dm
CHICKEN:GGA.2117.1.S1_AT WD repeat domain 5 gga
CHICKEN:GGAAFFX.12591.1.S1_AT WD repeat domain 5 gga
CHICKEN:GGAAFFX.12591.1.S1_S_AT WD repeat domain 5 gga
HU35KSUBA:AA147144_AT WD repeat domain 5 hs
HU35KSUBD:RC_AA421266_AT WD repeat domain 5 hs
U133_X3P:HS.323518.0.S1_3P_S_AT WD repeat domain 5 hs
U133_X3P:HS.13889.0.S1_3P_X_AT WD repeat domain 5 hs
U133_X3P:HS.13889.0.S1_3P_AT WD repeat domain 5 hs
HG-U133_PLUS_2:223308_S_AT WD repeat domain 5 hs
HG-U133B:223308_S_AT WD repeat domain 5 hs
HG-U133_PLUS_2:225170_AT WD repeat domain 5 hs
HG-U133B:225170_AT WD repeat domain 5 hs
HG-U95B:43017_AT WD repeat domain 5 hs
HG-U95B:43018_G_AT WD repeat domain 5 hs
HG-U95B:43410_AT WD repeat domain 5 hs
HG-U95B:43411_G_AT WD repeat domain 5 hs
HG-U95E:78481_AT WD repeat domain 5 hs
HG-U95E:89596_AT WD repeat domain 5 hs
MU11KSUBA:AA039038_S_AT WD repeat domain 5 mm
MG-U74AV2:97845_AT WD repeat domain 5 mm
MOE430A:1416581_AT WD repeat domain 5 mm
MOUSE430A_2:1416581_AT WD repeat domain 5 mm
MOUSE430_2:1416581_AT WD repeat domain 5 mm
MOE430A:1448389_AT WD repeat domain 5 mm
MOUSE430A_2:1448389_AT WD repeat domain 5 mm
MOUSE430_2:1448389_AT WD repeat domain 5 mm
MOUSE430_2:1446644_AT WD repeat domain 5, mRNA (cDNA clone MGC:36316 IMAGE:5132525) mm
MOE430B:1446644_AT WD repeat domain 5, mRNA (cDNA clone MGC:36316 IMAGE:5132525) mm
MU19KSUBA:TC16820_AT WD repeat domain 5, mRNA (cDNA clone MGC:36316 IMAGE:5132525) mm
MU19KSUBA:TC21615_AT WD repeat domain 5, mRNA (cDNA clone MGC:36316 IMAGE:5132525) mm
MU19KSUBB:TC29038_AT WD repeat domain 5, mRNA (cDNA clone MGC:36316 IMAGE:5132525) mm
MU19KSUBB:TC31423_AT WD repeat domain 5, mRNA (cDNA clone MGC:36316 IMAGE:5132525) mm
RAE230A:1388897_AT WD repeat domain 5 (predicted) rn
RAT230_2:1388897_AT WD repeat domain 5 (predicted) rn
RAE230B:1383297_AT WD repeat domain 5 (predicted) rn
RAT230_2:1383297_AT WD repeat domain 5 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA85487 Hypothetical protein C14B1.4 [Caenorhabditis elegans] ref|NP_497749.1| C14B1.4 [Caenorhabditis elegans] sp|Q17963|YKY4_CAEEL Hypothetical WD-repeat protein C14B1.4 in chromosome III 0.0
blast CAB63431 Hypothetical protein C14B1.10 [Caenorhabditis elegans] ref|NP_497751.1| C14B1.10 [Caenorhabditis elegans] 0.0
blast CAA85493 Hypothetical protein C14B1.5 [Caenorhabditis elegans] ref|NP_497750.1| C14B1.5 [Caenorhabditis elegans] sp|P49958|YKY5_CAEEL Hypothetical protein C14B1.5 in chromosome III 0.0
blast CAE64482 Hypothetical protein CBG09206 [Caenorhabditis briggsae] 1.0E-157
blast CAB72292 EG:BACR25B3.7 [Drosophila melanogaster] gb|AAF45791.1| CG17437-PA [Drosophila melanogaster] gb|AAM48415.1| RE31658p [Drosophila melanogaster] ref|NP_524984.1| CG17437-PA [Drosophila melanogaster] gb|AAF43418.1| WDS [Drosophila melanogaster] sp|Q9V3J8|WDS_DROME Will die slowly protein 1.0E-131
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.5E-12
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 7.2E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.8E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.7E-14
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.5E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.5E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.022
Pfam IPR002728 EMBL-EBI Diphthamide synthesis DPH2 protein 1.0E-126
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 1.1E-10
Sequence
Target Sequence 
>C. ELEGANS:192784_AT
acaataactacgtcttctgctgcaatttcaacccacaatcatcgctcgttgtctccggat
cattcgatgaatccgttcgtatctgggatgtcaagacaggaatgtgcataaaaaccctcc
cagcacactcagatccagtgtcagcagtgtctttcaacagagacggcagcctcatcgcat
cgggatcctacgatggtctagttcgtatctgggacactgccaatgggcaatgcatcaaga
ctctggtcgacgacgagaatcctccggtggcatttgtaaagttctctccaaacggaaaat
acatcctcgcatcgaacctcgacagcactttgaaattgtgggatttctcgaaaggaaaaa
ccctgaaacaatacactggacacgagaatagcaaatattgcatcttcgccaacttctctg
ttaccggtggaaaatggatcatcagtggttccgaagattgtaagatctacatctggaatc
tccagactcgggaaatagttcaatgtcttgaaggacacacacaacccgtgctagcctccg
attgtcatccagttcagaacatcattgcctcaggagctctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAATAACTACGTCTTCTGCTGCAA 412 115 521 Antisense
TCAACCCACAATCATCGCTCGTTGT 253 629 548 Antisense
GAATGTGCATAAAAACCCTCCCAGC 40 333 620 Antisense
TCCAGTGTCAGCAGTGTCTTTCAAC 631 589 654 Antisense
CAACAGAGACGGCAGCCTCATCGCA 323 189 675 Antisense
GTATCTGGGACACTGCCAATGGGCA 44 455 725 Antisense
GGGCAATGCATCAAGACTCTGGTCG 545 491 745 Antisense
AAAATACATCCTCGCATCGAACCTC 637 133 816 Antisense
ATCGAACCTCGACAGCACTTTGAAA 224 37 831 Antisense
AAATATTGCATCTTCGCCAACTTCT 452 115 913 Antisense
GAACATCATTGCCTCAGGAGCTCTC 229 351 1077 Antisense
Sequence Source Affymetrix Proprietary Database