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[192760_at] potassium channel protein

Description M04B2.5 /REP_DB=WormBase Gene ID /WP=CE12392 /GEN=twk-13 /TR=Q21505 /GB=CAB01238.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 60      4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_highest_nonorm > 61      6grp_total > 30      4grp_total > 04      6grp_highest_sub > 04      6grp_cgc > 73      6grp_high1_sub > 04      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 353.3
(-0.5)
P 		228.2
(-3)
P 		236.7
(-2.8)
P 		529
(3)
P 		301
B 290.6
(-3)
P 		297.5
(-2.8)
P 		305
(-2.6)
P 		530.1
(3)
P 		240
C 370
(0.3)
P 		229.8
(-3)
P 		279.4
(-1.8)
P 		483.5
(3)
P 		254
avr 338
(-1) 		251.8
(-3) 		273.7
(-2.5) 		514.2
(3) 		262

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 405
1 2 3 4 5 6 range
A 353.7
(-1)
P 		522
(1.9)
P 		461
(0.8)
P 		587.7
(3)
P 		352.4
(-1)
P 		239.1
(-3)
P 		349
B 373.9
(0.2)
P 		511.4
(2.6)
P 		531.2
(3)
P 		528.4
(2.9)
P 		316.5
(-0.8)
P 		195.4
(-3)
P 		336
C 409.9
(-0)
P 		548.4
(2.2)
P 		494.3
(1.3)
P 		600.6
(3)
P 		340.4
(-1.1)
P 		224
(-3)
P 		377
avr 379.2
(-0.3) 		527.3
(2.2) 		495.5
(1.7) 		572.2
(3) 		336.4
(-1) 		219.5
(-3) 		353
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 128.6
(3)
P 		49.5
(-3)
P 		61.9
(-2.1)
P 		77.4
(-0.9)
P 		79
B 92.7
(0.1)
P 		73.5
(-2.6)
P 		70.7
(-3)
P 		113
(3)
P 		42
C 122.5
(3)
P 		66.1
(-3)
P 		97.4
(0.3)
P 		81.5
(-1.4)
P 		56
avr 114.6
(3) 		63
(-3) 		76.7
(-1.4) 		90.6
(0.2) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 65.2
(-2.7)
P 		185.7
(3)
P 		100.4
(-1)
P 		149.1
(1.3)
P 		106.3
(-0.7)
P 		58.2
(-3)
P 		128
B 130.1
(0.5)
P 		170.2
(3)
P 		129
(0.5)
P 		140
(1.1)
P 		128.6
(0.4)
P 		72.9
(-3)
P 		97
C 151.9
(2)
P 		167.3
(3)
P 		125.8
(0.3)
P 		120.8
(-0)
P 		110.2
(-0.7)
P 		75
(-3)
P 		92
avr 115.7
(-0.3) 		174.4
(3) 		118.4
(-0.2) 		136.6
(0.9) 		115
(-0.4) 		68.7
(-3) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192760_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10185
Sequence Type Exemplar sequence
Representative Public ID M04B2.5 NCBI
Target Description M04B2.5 /REP_DB=WormBase Gene ID /WP=CE12392 /GEN=twk-13 /TR=Q21505 /GB=CAB01238.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069769(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069769 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-25) (twk-25) mRNA, complete cds. 11/11 None
SNAP00000026175 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11506613:11509543:1 11/11 None
GENEFINDER00000026186 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11505025:11509543:1 11/11 None
M04B2.5 ENSEMBL cdna:known chromosome:CEL140:IV:11506435:11509543:1 gene:M04B2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11506440-11509549(+) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein
Gene Symbol twk-25
Ensembl M04B2.5
Entrez Gene 192076 Entrez gene
SwissProt Q21505 EMBL-EBI
WormBase CE36554 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01238 Hypothetical protein M04B2.5 [Caenorhabditis elegans] ref|NP_502170.2| TWiK family of potassium channels family member (twk-25) [Caenorhabditis elegans] 0.0
blast CAE62108 Hypothetical protein CBG06143 [Caenorhabditis briggsae] 0.0
blast CAE61731 Hypothetical protein CBG05682 [Caenorhabditis briggsae] 1.0E-114
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502170.2 7 136-158,238-260,265-284,288-310,322-344,359-378,385-402
M04B2.5 7 136-158,238-260,265-284,288-310,322-344,359-378,385-402
SNAP00000026175 7 115-137,217-239,244-263,267-289,301-323,338-357,364-381
GENEFINDER00000026186 6 231-253,333-355,365-384,417-439,454-473,480-497
Sequence
Target Sequence 
>C. ELEGANS:192760_AT
atgatattatacttactctccgctacaatgtttatattcgaatacgacgaactgtctgga
ccacctgattctggaatttcatttttccatgctttctacttttcattcatttcaatgtca
actatcggtttaggagatgttatgccaaataatgtaacattctctccattaatcactatt
atgtttttctttggaatgccgattcttaaagttgtcaatagagttacgtatatttgtctg
gaaaatggagtttttggatcgatgacagtgcttgaaaatcgactagataccatttggagc
aaggcaacagtattaccaacaggccaagtgatttctgagccaccaacgatagatgttgca
agtcggaaaacgtcgatgttgagtgaaggaatgattccagatgaaaacgatggttcggtt
ccaaatgaatacctgaacaacttcacaattcgttccattgcaacattcatgaaagccaac
ggagatgtatacggaggtgcatttggtcgtgtgaatatccgtcgaggtgacatacggaac
ccagggtctgctgctgataatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATATTATACTTACTCTCCGCTA 196 47 1027 Antisense
CTTACTCTCCGCTACAATGTTTATA 222 221 1038 Antisense
ATACGACGAACTGTCTGGACCACCT 339 15 1068 Antisense
TTTTCCATGCTTTCTACTTTTCATT 484 677 1109 Antisense
GTAACATTCTCTCCATTAATCACTA 703 461 1180 Antisense
GGCCAAGTGATTTCTGAGCCACCAA 94 545 1348 Antisense
ACAACTTCACAATTCGTTCCATTGC 696 111 1463 Antisense
TACGGAGGTGCATTTGGTCGTGTGA 694 647 1516 Antisense
GGTCGTGTGAATATCCGTCGAGGTG 245 499 1531 Antisense
GAGGTGACATACGGAACCCAGGGTC 584 399 1550 Antisense
AACCCAGGGTCTGCTGCTGATAATC 78 147 1564 Antisense
Sequence Source Affymetrix Proprietary Database