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[192754_at]

Description F12A10.3 /REP_DB=WormBase Gene ID /WP=CE01906 /TR=Q09945 /GB=AAA68296.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=aromatic-L-amino-acid decarboxylase
Cluster 6grp_total > 85      4grp_total > 01      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 2.4
(-3)
A 		2.6
(-3)
A 		3.3
(-2.9)
A 		54.9
(3)
A 		53
B 6.8
(3)
A 		3.9
(-1.4)
A 		3.3
(-2.3)
A 		2.8
(-3)
A 		4
C 4.1
(-2.8)
A 		2.8
(-3)
A 		13.2
(-1)
A 		34.2
(3)
A 		31
avr 4.4
(-2.7) 		3.1
(-3) 		6.6
(-2.2) 		30.6
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 19.9
(0.6)
A 		5.3
(-2.5)
A 		3.9
(-2.8)
A 		31.1
(3)
A 		3
(-3)
A 		16
(-0.2)
A 		28
B 14.1
(-1)
A 		4.5
(-2.6)
A 		1.8
(-3)
A 		33.6
(2.1)
A 		34.7
(2.3)
A 		38.9
(3)
A 		37
C 2.9
(-2.7)
A 		3.3
(-2.7)
A 		1.4
(-3)
A 		1.5
(-3)
A 		9.2
(-1.6)
A 		35.7
(3)
A 		34
avr 12.3
(-0.9) 		4.4
(-2.6) 		2.4
(-3) 		22.1
(1.3) 		15.6
(-0.2) 		30.2
(3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 0.9
(-2.8)
A 		0.6
(-3)
A 		0.9
(-2.8)
A 		8
(3)
A 		7
B 2.2
(3)
A 		1
(-1.5)
A 		0.8
(-2.3)
A 		0.6
(-3)
A 		2
C 1.3
(-2.4)
A 		0.8
(-3)
A 		4.6
(1.6)
A 		5.8
(3)
A 		5
avr 1.5
(-2) 		0.8
(-3) 		2.1
(-1.1) 		4.8
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 3.7
(-0.5)
A 		1.9
(-2.1)
A 		0.8
(-3)
A 		7.9
(3)
A 		0.9
(-2.9)
A 		3.9
(-0.4)
A 		7
B 4.9
(-1.1)
A 		1.5
(-2.5)
A 		0.4
(-3)
A 		8.9
(0.6)
A 		14.1
(2.8)
A 		14.5
(3)
A 		14
C 1.1
(-2.6)
A 		1
(-2.6)
A 		0.4
(-2.9)
A 		0.3
(-3)
A 		3
(-1.6)
A 		12
(3)
A 		12
avr 3.2
(-1.3) 		1.5
(-2.4) 		0.5
(-3) 		5.7
(0.3) 		6
(0.4) 		10.1
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192754_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4531
Sequence Type Exemplar sequence
Representative Public ID F12A10.3 NCBI
Target Description F12A10.3 /REP_DB=WormBase Gene ID /WP=CE01906 /TR=Q09945 /GB=AAA68296.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=aromatic-L-amino-acid decarboxylase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062644(11), AY669392(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062644 NCBI Caenorhabditis elegans F12A10.3 (F12A10.3) mRNA, complete cds. 11/11 None
AY669392 NCBI Caenorhabditis elegans truncated aromatic L-amino acid decarboxylase (F12A10.3) mRNA, complete cds, alternatively spliced. 10/11 None
GENEFINDER00000033536 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5491081:5493662:1 10/11 None
F12A10.3 ENSEMBL cdna:known chromosome:CEL140:II:5491843:5493135:1 gene:F12A10.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033520 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5491078-5493160(+) 100.0 100.0
Public Domain and Genome References
Ensembl F12A10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T16041 hypothetical protein F12A10.3 - Caenorhabditis elegans 0.0
blast AAC02720 Hypothetical protein C05D2.3 [Caenorhabditis elegans] ref|NP_498210.1| C05D2.3 [Caenorhabditis elegans] 1.0E-135
blast NP_495045 F12A10.3 [Caenorhabditis elegans] 1.0E-133
blast AAV58886 Biogenic amine synthesis related protein 1, isoform a [Caenorhabditis elegans] ref|NP_001021150.1| Biogenic Amine Synthesis related family member (bas-1) [Caenorhabditis elegans] 1.0E-130
blast T16041 hypothetical protein F12A10.3 - Caenorhabditis elegans 1.0E-108
blast AAU05397 truncated aromatic L-amino acid decarboxylase [Caenorhabditis elegans] gb|AAU05396.1| truncated aromatic L-amino acid decarboxylase [Caenorhabditis elegans] 3.0E-93
blast AAC02720 Hypothetical protein C05D2.3 [Caenorhabditis elegans] ref|NP_498210.1| C05D2.3 [Caenorhabditis elegans] 4.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 6.1E-28
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 1.7E-31
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 4.7E-32
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-23
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 7.2E-29
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 5.6E-21
Sequence
Target Sequence 
>C. ELEGANS:192754_AT
ggactttatgtacacgtggattcggcttatgctggtatttcagtttttcggaaaaaccct
acaattcaaactattaaaggttcgtatgcactttgtgaagagaatagatatttggtgcgc
ggagttgaagacgtggattcatacaacacaaatcttcataaagctggaatgattaatttt
gactgctcaccaatgtggtttaagaatggaatctatgcatctcgatactacaatgtgaat
cctgttcacttggttcatgaatatcaggcatctgcgacagattacagacatcttgaaatt
ccattgggacgtcgcttccgttctcttaaactttggtttacactgagaaactggggagtc
gaaaatatcaaaaattacatgagacaggtagaaagggaagaacattcaaatgcagaaaat
gaacgtttgtgtaatgcaattaacaaggattgtcgtatccacataattccatcatcggtt
catgatgtcttccttcttcgttttgctgttggaagtccgatgagcaacattggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACTTTATGTACACGTGGATTCGG 258 523 796 Antisense
GATTCGGCTTATGCTGGTATTTCAG 171 431 814 Antisense
GGTGCGCGGAGTTGAAGACGTGGAT 679 501 909 Antisense
TGATTAATTTTGACTGCTCACCAAT 54 565 965 Antisense
GGAATCTATGCATCTCGATACTACA 279 533 1003 Antisense
GAATCCTGTTCACTTGGTTCATGAA 176 331 1032 Antisense
GAAATTCCATTGGGACGTCGCTTCC 185 363 1090 Antisense
TTGTCGTATCCACATAATTCCATCA 703 705 1245 Antisense
CATCGGTTCATGATGTCTTCCTTCT 221 213 1268 Antisense
TCCTTCTTCGTTTTGCTGTTGGAAG 624 589 1286 Antisense
GGAAGTCCGATGAGCAACATTGGTG 89 531 1306 Antisense
Sequence Source Affymetrix Proprietary Database