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[192743_s_at] Rho-associated kinase

Description C10H11.9 /REP_DB=WormBase Gene ID /WP=CE08098 /GEN=let-502 /TR=P92199 /GB=AAB42348.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=Rho-associated kinase
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_high_8_8 > 49      6grp_highest_nonorm > 81      6grp_total > 75      4grp_total > 45      6grp_cgc > 66      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 339.8
(3)
P 		248.7
(-0.6)
P 		188.7
(-3)
A 		196.8
(-2.7)
P 		151
B 270.5
(-1.9)
P 		299.3
(3)
P 		277.7
(-0.7)
P 		263.9
(-3)
P 		35
C 369.7
(3)
P 		333.3
(-0.9)
P 		314.2
(-3)
P 		316.4
(-2.8)
P 		56
avr 326.7
(3) 		293.8
(0.1) 		260.2
(-2.9) 		259
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 225
1 2 3 4 5 6 range
A 312.7
(-3)
P 		402.7
(0.6)
P 		335.4
(-2.1)
P 		365.5
(-0.9)
P 		342.5
(-1.8)
P 		461
(3)
P 		148
B 270.1
(-0.8)
P 		247.3
(-3)
P 		262.4
(-1.6)
P 		309.9
(3)
P 		267.3
(-1.1)
P 		295.7
(1.6)
P 		63
C 341.8
(1.2)
P 		236.5
(-3)
P 		387.9
(3)
P 		344.5
(1.3)
P 		314
(0.1)
P 		264.5
(-1.9)
P 		151
avr 308.2
(-1.3) 		295.5
(-3) 		328.6
(1.4) 		340
(2.9) 		307.9
(-1.3) 		340.4
(3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 123.7
(3)
P 		54
(-1.4)
P 		49.3
(-1.7)
A 		28.8
(-3)
P 		95
B 86.3
(3)
P 		73.9
(0.5)
P 		64.3
(-1.4)
P 		56.2
(-3)
P 		30
C 122.4
(3)
P 		95.8
(0.7)
P 		109.5
(1.9)
P 		53.4
(-3)
P 		69
avr 110.8
(3) 		74.6
(-0.4) 		74.4
(-0.4) 		46.1
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 57.6
(-3)
P 		143.2
(3)
P 		73
(-1.9)
P 		92.7
(-0.5)
P 		103.3
(0.2)
P 		112.2
(0.8)
P 		86
B 94
(0.9)
P 		82.3
(-0.6)
P 		63.7
(-3)
P 		82.1
(-0.6)
P 		108.6
(2.8)
P 		110.3
(3)
P 		47
C 126.7
(3)
P 		72.2
(-2.7)
P 		98.7
(0.1)
P 		69.3
(-3)
P 		101.6
(0.4)
P 		88.5
(-1)
P 		57
avr 92.8
(0.3) 		99.2
(1.8) 		78.5
(-3) 		81.4
(-2.3) 		104.5
(3) 		103.7
(2.8) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192743_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.781
Sequence Type Exemplar sequence
Representative Public ID C10H11.9 NCBI
Target Description C10H11.9 /REP_DB=WormBase Gene ID /WP=CE08098 /GEN=let-502 /TR=P92199 /GB=AAB42348.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=Rho-associated kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059039(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059039 NCBI Caenorhabditis elegans LEThal family member (let-502) (let-502) mRNA, complete cds. 11/11 A 
SNAP00000015956 ENSEMBL cdna:SNAP chromosome:CEL140:I:4738956:4744262:-1 11/11 None
GENEFINDER00000015961 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4738956:4747592:-1 11/11 None
C10H11.9 ENSEMBL cdna:known chromosome:CEL140:I:4738171:4744310:-1 gene:C10H11.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4738955-4744262(-) 100.0 100.0
Public Domain and Genome References
Gene Title Rho-associated kinase
Gene Symbol let-502
Ensembl C10H11.9
Entrez Gene 172088 Entrez gene
SwissProt P92199 EMBL-EBI
WormBase CE08098 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.6269.1.S1_S_AT similar to Rho-associated protein kinase 2 (Rho-associated, coiled-coil containing protein kinase 2) (p164 ROCK-2) (Rho kinase 2) cfa
CHICKEN:GGA.17943.1.S1_AT Rho-associated, coiled-coil containing protein kinase 2 gga
CHICKEN:GGA.3500.1.S1_AT Rho-associated, coiled-coil containing protein kinase 2 gga
CHICKEN:GGAAFFX.10528.1.S1_AT Rho-associated, coiled-coil containing protein kinase 2 gga
CHICKEN:GGAAFFX.10529.1.S1_AT Rho-associated, coiled-coil containing protein kinase 2 gga
HG-U133_PLUS_2:211504_X_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-U133A:211504_X_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-U133A_2:211504_X_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HU35KSUBC:RC_F13712_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HU35KSUBD:RC_AA287360_AT Rho-associated, coiled-coil containing protein kinase 2 hs
U133_X3P:G4520224_3P_A_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HU35KSUBB:RC_AA228121_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HU35KSUBC:RC_AA133756_AT Rho-associated, coiled-coil containing protein kinase 2 hs
U133_X3P:HS.58617.0.S2_3P_X_AT Rho-associated, coiled-coil containing protein kinase 2 hs
U133_X3P:HS.58617.0.S2_3P_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-U133A_2:202762_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-FOCUS:202762_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-U133_PLUS_2:202762_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-U133A:202762_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HG-U95AV2:35335_AT Rho-associated, coiled-coil containing protein kinase 2 hs
HU35KSUBB:RC_R08165_AT Rho-associated, coiled-coil containing protein kinase 2 hs
MU11KSUBA:AA423575_S_AT Rho-associated coiled-coil forming kinase 2 mm
MU11KSUBB:MSA.2966.0_S_AT Rho-associated coiled-coil forming kinase 2 mm
MG-U74AV2:98504_AT Rho-associated coiled-coil forming kinase 2 mm
MOE430A:1423592_AT Rho-associated coiled-coil forming kinase 2 mm
MOUSE430A_2:1423592_AT Rho-associated coiled-coil forming kinase 2 mm
MOUSE430_2:1423592_AT Rho-associated coiled-coil forming kinase 2 mm
MOE430A:1451041_AT Rho-associated coiled-coil forming kinase 2 mm
MOUSE430A_2:1451041_AT Rho-associated coiled-coil forming kinase 2 mm
MOUSE430_2:1451041_AT Rho-associated coiled-coil forming kinase 2 mm
MU19KSUBB:TC31219_AT Rho-associated coiled-coil forming kinase 2 (Rock2), mRNA mm
MU19KSUBC:TC37940_AT Rho-associated coiled-coil forming kinase 2 (Rock2), mRNA mm
RG-U34A:U38481_AT Rho-associated coiled-coil forming kinase 2 rn
RAE230A:1387379_AT Rho-associated coiled-coil forming kinase 2 rn
RAT230_2:1387379_AT Rho-associated coiled-coil forming kinase 2 rn
RAE230A:1372371_AT Rho-associated coiled-coil forming kinase 2 rn
RAT230_2:1372371_AT Rho-associated coiled-coil forming kinase 2 rn
RAE230B:1382512_AT Rho-associated coiled-coil forming kinase 2 rn
RAT230_2:1382512_AT Rho-associated coiled-coil forming kinase 2 rn
RG-U34B:RC_AA926209_AT Rho-associated coiled-coil forming kinase 2 rn
RG-U34B:RC_AI009363_AT Rho-associated coiled-coil forming kinase 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from sequence similarity QuickGO AmiGO
5524 ATP binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42348 Lethal protein 502 [Caenorhabditis elegans] ref|NP_491440.1| LEThal family member (let-502) [Caenorhabditis elegans] gb|AAB42081.1| LET-502 [Caenorhabditis elegans] 0.0
blast CAE60362 Hypothetical protein CBG03960 [Caenorhabditis briggsae] 0.0
blast BAA31594.2 KIAA0619 protein [Homo sapiens] 0.0
Protein Families
Method ID Description E-Value
hanks 1.9.1 AGC group; AGC Other; DM 5.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 5.3E-72
Pfam IPR000719 EMBL-EBI Protein kinase 5.3E-72
Sequence
Target Sequence 
>C. ELEGANS:192743_S_AT
tggaccaactgctttgttgcgcttaacgagtattcattcacaatctatgtcgacgaaaaa
gccgtgtctgtgattcttttgattgaagccggagctatggctcatgttcgacacgtcact
gctgctgatttgagaaatgtcgacgatagtcagttacctaagatattccatatcatgtat
gacgacgcgtcgtgcaattcgtcacgacacgcctccaattcagatctctcaatgattgaa
tcgttccgagaagaaagttggaagcggcacgatttccaagagctctcctatcacatacgc
acgttttgtgatgtctgcaataagaaattgtctgacattatccgaccgacgcctgcattc
gaatgcaaaaattgccatttcaagacacacaaggatcatgttgctcaaggatcgttgcca
atgtgtcgatacaacacgggtctctctcgtgaactcgttcttatggctccacaaacggat
aactgcaataaatgggtatcacaacttcgacgcttcattgaatcatcacgatcagcggca
gtttcagtgtctcgtgtctcttcacgacgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGACCAACTGCTTTGTTGCGCTTA 156 549 2923 Antisense
AGCCGTGTCTGTGATTCTTTTGATT 585 83 2982 Antisense
ACACGTCACTGCTGCTGATTTGAGA 249 109 3033 Antisense
GACGACGCGTCGTGCAATTCGTCAC 692 377 3103 Antisense
ATTTCCAAGAGCTCTCCTATCACAT 523 17 3194 Antisense
TTGTCTGACATTATCCGACCGACGC 201 707 3250 Antisense
ACAACACGGGTCTCTCTCGTGAACT 353 111 3353 Antisense
AACTCGTTCTTATGGCTCCACAAAC 587 141 3374 Antisense
ATCACAACTTCGACGCTTCATTGAA 603 27 3420 Antisense
ATCAGCGGCAGTTTCAGTGTCTCGT 260 27 3453 Antisense
TGTCTCGTGTCTCTTCACGACGACA 703 557 3470 Antisense
Sequence Source Affymetrix Proprietary Database