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[192739_at]

Description B0334.2 /REP_DB=WormBase Gene ID /WP=CE02933 /GEN=twk-1 /TR=Q17481 /GB=CAA91376.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 89      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 91      6grp_total > 99      4grp_total > 02      6grp_highest_sub > In      6grp_cgc > 79      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2950
1 2 3 4 range
A 254.9
(-2.6)
P 		81.5
(-3)
P 		131.8
(-2.9)
P 		2502.2
(3)
P 		2421
B 277.6
(-2.7)
P 		163.9
(-2.9)
P 		107.7
(-3)
P 		3031.9
(3)
P 		2924
C 262.1
(-2.7)
P 		142.4
(-2.9)
P 		111.1
(-3)
P 		2808.5
(3)
P 		2697
avr 264.9
(-2.7) 		129.3
(-3) 		116.9
(-3) 		2780.9
(3) 		2664

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 170.3
(-2.2)
P 		158.4
(-3)
P 		202.2
(-0.2)
P 		160.8
(-2.8)
P 		253
(3)
P 		247.2
(2.6)
P 		95
B 225.7
(-0.6)
P 		172.6
(-3)
P 		185.6
(-2.4)
P 		180.7
(-2.6)
P 		307.6
(3)
P 		303.8
(2.8)
P 		135
C 224.2
(-2)
P 		213.5
(-2.6)
P 		205.7
(-3)
P 		242.1
(-1.1)
P 		300.9
(2)
P 		319.1
(3)
P 		113
avr 206.7
(-1.6) 		181.5
(-3) 		197.8
(-2.1) 		194.5
(-2.3) 		287.2
(2.8) 		290
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 628
1 2 3 4 range
A 92.8
(-1.7)
P 		17.7
(-3)
P 		34.4
(-2.7)
P 		366.3
(3)
P 		349
B 88.5
(-2.4)
P 		40.5
(-2.9)
P 		25
(-3)
P 		646.1
(3)
P 		621
C 86.7
(-2.3)
P 		40.9
(-3)
P 		38.7
(-3)
P 		473.6
(3)
P 		435
avr 89.3
(-2.3) 		33
(-3) 		32.7
(-3) 		495.3
(3) 		463

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 31.4
(-3)
P 		56.3
(0.3)
P 		44
(-1.3)
P 		40.8
(-1.7)
P 		76.3
(3)
P 		60.2
(0.8)
P 		45
B 78.5
(-0.5)
P 		57.5
(-2.1)
P 		45.1
(-3)
P 		47.9
(-2.8)
P 		125
(3)
P 		113.3
(2.1)
P 		80
C 83.1
(0.6)
P 		65.2
(-1.3)
P 		52.4
(-2.6)
P 		48.7
(-3)
P 		97.4
(2)
P 		106.8
(3)
P 		58
avr 64.3
(-0.9) 		59.7
(-1.4) 		47.2
(-2.8) 		45.8
(-3) 		99.6
(3) 		93.4
(2.3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192739_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3973
Sequence Type Exemplar sequence
Representative Public ID B0334.2 NCBI
Target Description B0334.2 /REP_DB=WormBase Gene ID /WP=CE02933 /GEN=twk-1 /TR=Q17481 /GB=CAA91376.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026725(11), NM_001026724(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026725 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-5) (twk-5) mRNA, complete cds. 11/11 None
NM_001026724 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-5) (twk-5) mRNA, complete cds. 11/11 None
SNAP00000019010 ENSEMBL cdna:SNAP chromosome:CEL140:II:11483193:11485121:-1 11/11 None
GENEFINDER00000019031 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11483193:11485121:-1 11/11 None
B0334.2b ENSEMBL cdna:known chromosome:CEL140:II:11483126:11485215:-1 gene:B0334.2 11/11 None
B0334.2a ENSEMBL cdna:known chromosome:CEL140:II:11483193:11484752:-1 gene:B0334.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11483189-11484749(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0334.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91376 Hypothetical protein B0334.2a [Caenorhabditis elegans] ref|NP_001021895.1| TWiK family of potassium channels family member (twk-5) [Caenorhabditis elegans] 1.0E-158
blast CAI46547 Hypothetical protein B0334.2b [Caenorhabditis elegans] ref|NP_001021896.1| TWiK family of potassium channels family member (twk-5) [Caenorhabditis elegans] 1.0E-152
blast CAA91376 Hypothetical protein B0334.2a [Caenorhabditis elegans] ref|NP_001021895.1| TWiK family of potassium channels family member (twk-5) [Caenorhabditis elegans] 1.0E-148
blast CAI46547 Hypothetical protein B0334.2b [Caenorhabditis elegans] ref|NP_001021896.1| TWiK family of potassium channels family member (twk-5) [Caenorhabditis elegans] 1.0E-148
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021896.1 5 13-35,50-67,74-96,127-149,185-207
NP_001021895.1 5 20-42,54-71,81-103,136-158,192-214
B0334.2b 5 13-35,50-67,74-96,127-149,185-207
B0334.2a 5 20-42,54-71,81-103,136-158,192-214
SNAP00000019010 5 13-35,50-67,74-96,127-149,185-207
GENEFINDER00000019031 5 13-35,50-67,74-96,127-149,185-207
Sequence
Target Sequence 
>C. ELEGANS:192739_AT
cacgagttttctcaatattcttctcaattttggggattcctcttcttgtagtgacccttg
gaaattttggaaaatatttgacaaaattctactggaaaactcatggttggattttctcgg
aaagaaccgaaagcgaactagtaaatgacaaggatatgccaggtatcgtaatcgcctgcc
tctaccttctaacatttgcaattggcttcttctttattccacactcgggagctgcgtatt
ccattgatgactgctatttcagcttcatcagttttgcaacagttggtttcggagacaaag
tgcctcaaatcgatacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGAGTTTTCTCAATATTCTTCTC 699 199 257 Antisense
TTCTCAATTTTGGGGATTCCTCTTC 624 689 277 Antisense
TCCTCTTCTTGTAGTGACCCTTGGA 319 593 294 Antisense
AACTCATGGTTGGATTTTCTCGGAA 633 139 354 Antisense
TGCCAGGTATCGTAATCGCCTGCCT 275 585 413 Antisense
CCTCTACCTTCTAACATTTGCAATT 702 267 435 Antisense
GCAATTGGCTTCTTCTTTATTCCAC 549 323 454 Antisense
CTTCTTTATTCCACACTCGGGAGCT 450 219 465 Antisense
GAGCTGCGTATTCCATTGATGACTG 253 387 485 Antisense
ATTTCAGCTTCATCAGTTTTGCAAC 587 15 512 Antisense
GACAAAGTGCCTCAAATCGATACAT 110 365 550 Antisense
Sequence Source Affymetrix Proprietary Database