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[192734_at] guanine-nucleotide releasing factor

Description F28B4.2 /REP_DB=WormBase Gene ID /WP=CE28222 /TR=Q19852 /GB=AAA83583.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanine-nucleotide releasing factor
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 20      4grp_high_8_8 > 21      6grp_highest_nonorm > 81      6grp_total > 50      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 266.8
(-3)
P 		333.7
(0.5)
P 		273.2
(-2.7)
P 		381.3
(3)
A 		115
B 270.1
(1.1)
P 		223.1
(-3)
P 		259.7
(0.2)
P 		291.3
(3)
P 		68
C 274.7
(-3)
P 		286.6
(3)
A 		278.2
(-1.2)
P 		279.2
(-0.7)
M 		12
avr 270.5
(-3) 		281.1
(-1.6) 		270.4
(-3) 		317.3
(3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 260.3
(-1.8)
P 		347.1
(3)
P 		319.6
(1.5)
P 		256.5
(-2)
P 		239.2
(-3)
P 		293.2
(0)
P 		108
B 328.8
(3)
P 		248.5
(-0.7)
P 		321.2
(2.7)
P 		319.4
(2.6)
P 		197.4
(-3)
P 		279.3
(0.7)
P 		131
C 260.9
(-0.9)
P 		252.5
(-1.3)
P 		263.7
(-0.7)
P 		336.3
(3)
P 		219.3
(-3)
P 		259.4
(-0.9)
P 		117
avr 283.3
(1.5) 		282.7
(1.5) 		301.5
(2.8) 		304.1
(3) 		218.6
(-3) 		277.3
(1.1) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 97.2
(3)
P 		72.4
(-0.6)
P 		71.4
(-0.7)
P 		55.8
(-3)
A 		41
B 86.1
(3)
P 		55.1
(-3)
P 		60.2
(-2)
P 		62.1
(-1.6)
P 		31
C 90.9
(2.3)
P 		82.4
(1.2)
A 		97
(3)
P 		47.1
(-3)
M 		50
avr 91.4
(3) 		70
(-0.5) 		76.2
(0.5) 		55
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 48
(-3)
P 		123.4
(3)
P 		69.6
(-1.3)
P 		65
(-1.6)
P 		72.2
(-1.1)
P 		71.4
(-1.1)
P 		75
B 114.4
(3)
P 		82.7
(-2.2)
P 		78
(-3)
P 		84.6
(-1.9)
P 		80.2
(-2.6)
P 		104.2
(1.3)
P 		36
C 96.7
(3)
P 		77
(-1)
P 		67.1
(-3)
P 		67.6
(-2.9)
P 		71
(-2.2)
P 		86.8
(1)
P 		30
avr 86.4
(0.9) 		94.4
(3) 		71.6
(-3) 		72.4
(-2.8) 		74.5
(-2.2) 		87.5
(1.2) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192734_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18379
Sequence Type Exemplar sequence
Representative Public ID F28B4.2 NCBI
Target Description F28B4.2 /REP_DB=WormBase Gene ID /WP=CE28222 /TR=Q19852 /GB=AAA83583.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanine-nucleotide releasing factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076149(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076149 NCBI Caenorhabditis elegans F28B4.2 (F28B4.2) mRNA, complete cds. 11/11 None
SNAP00000013188 ENSEMBL cdna:SNAP chromosome:CEL140:X:3210967:3215282:-1 11/11 None
GENEFINDER00000013196 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3210967:3220282:-1 11/11 None
F28B4.2 ENSEMBL cdna:known chromosome:CEL140:X:3210464:3220358:-1 gene:F28B4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3210967-3215481(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine-nucleotide releasing factor
Ensembl F28B4.2
Entrez Gene 180611 Entrez gene
SwissProt Q19852 EMBL-EBI
WormBase CE28222 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1599891_AT similar to Ral guanine nucleotide dissociation stimulator-like 1 (RalGDS-like 1) cfa
CANINE_2:CFAAFFX.20738.1.S1_S_AT similar to Ral guanine nucleotide dissociation stimulator-like 1 (RalGDS-like 1) cfa
DROSOPHILA_2:1628726_S_AT Ral guanine nucleotide exchange factor 2 dm
DROSGENOME1:148729_AT Ral guanine nucleotide exchange factor 2 dm
CHICKEN:GGAAFFX.12757.1.S1_S_AT ral guanine nucleotide dissociation stimulator-like 1 gga
CHICKEN:GGAAFFX.2934.3.S1_S_AT ral guanine nucleotide dissociation stimulator-like 1 gga
HG-U95B:43715_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U133A:209568_S_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U133_PLUS_2:209568_S_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U133A_2:209568_S_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HU35KSUBB:RC_AA169173_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HU35KSUBC:RC_W94479_AT ral guanine nucleotide dissociation stimulator-like 1 hs
U133_X3P:G7682470_3P_A_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U95AV2:37539_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U95E:74957_AT ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U95C:49532_AT Ral guanine nucleotide dissociation stimulator-like 1 hs
HG-U95D:90041_AT Ral guanine nucleotide dissociation stimulator-like 1 hs
HU35KSUBD:RC_AA620828_AT Ral guanine nucleotide dissociation stimulator-like 1 hs
MOUSE430_2:1418535_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MOUSE430A_2:1418535_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MOE430A:1418535_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MU11KSUBA:U14103_S_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MG-U74AV2:92671_F_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MG-U74BV2:112484_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MG-U74AV2:161260_F_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MOE430A:1449124_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MOUSE430A_2:1449124_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MOUSE430_2:1449124_AT ral guanine nucleotide dissociation stimulator,-like 1 mm
MOUSE430_2:1440662_AT Ral guanine nucleotide dissociation stimulator,-like 1, mRNA (cDNA clone MGC:18430 IMAGE:4241244) mm
MOE430B:1440662_AT Ral guanine nucleotide dissociation stimulator,-like 1, mRNA (cDNA clone MGC:18430 IMAGE:4241244) mm
MU19KSUBA:TC15047_AT Ral guanine nucleotide dissociation stimulator,-like 1, mRNA (cDNA clone MGC:18430 IMAGE:4241244) mm
MU19KSUBB:TC31953_AT Ral guanine nucleotide dissociation stimulator,-like 1, mRNA (cDNA clone MGC:18430 IMAGE:4241244) mm
MU19KSUBB:TC31953_G_AT Ral guanine nucleotide dissociation stimulator,-like 1, mRNA (cDNA clone MGC:18430 IMAGE:4241244) mm
RAT230_2:1394472_AT Ral guanine nucleotide dissociation stimulator,-like 1 (predicted) rn
RAE230B:1394472_AT Ral guanine nucleotide dissociation stimulator,-like 1 (predicted) rn
RAE230B:1395145_AT Ral guanine nucleotide dissociation stimulator,-like 1 (predicted) rn
RAT230_2:1395145_AT Ral guanine nucleotide dissociation stimulator,-like 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5085 guanyl-nucleotide exchange factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83583 Hypothetical protein F28B4.2 [Caenorhabditis elegans] ref|NP_508550.1| F28B4.2 [Caenorhabditis elegans] 0.0
blast CAE68393 Hypothetical protein CBG14158 [Caenorhabditis briggsae] 0.0
blast XP_393803 PREDICTED: similar to ENSANGP00000026631 [Apis mellifera] 4.0E-90
Protein Domains
Database ID Description E-Value
Pfam IPR000159 EMBL-EBI RA domain 1.9E-21
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 2.6E-39
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 3.4E-13
Pfam IPR000159 EMBL-EBI RA domain 1.9E-21
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 2.6E-39
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 3.4E-13
Sequence
Target Sequence 
>C. ELEGANS:192734_AT
aactagtggaacccaatcgctgtcccggagtgctacacctatcagaacgcgacttgccga
agtattcgattcgaaagtgttttcacatcagaaaaagggcagcgacgcttcaagttgctc
atcttcatcttctctctctgcaaatggaaattcgcaagcacaaaattcgttttatctagc
aagagtcggacttgacgatgatcttcagaatactgacggtgcaaattataaatgcattaa
gatagaaaatggagatcgaatgccgcagttggtagctcgagcgttggaaaaacatttgat
agaggatgataaaaatacgtatctgctcgtgcaacttttgccacgtggcggtgaatttgt
ccttcccgataattgcaacccattctatgcgatggctccggacccgacgtcgccgatgtt
aaacttattgttaagaaaacgtgacggaaacgggaccattgaaggtggtgcctcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTAGTGGAACCCAATCGCTGTCC 575 139 1986 Antisense
TCCCGGAGTGCTACACCTATCAGAA 710 597 2008 Antisense
ACCTATCAGAACGCGACTTGCCGAA 437 87 2022 Antisense
CAGCGACGCTTCAAGTTGCTCATCT 319 203 2085 Antisense
ATGCCGCAGTTGGTAGCTCGAGCGT 468 39 2245 Antisense
ATCTGCTCGTGCAACTTTTGCCACG 591 33 2306 Antisense
GCCACGTGGCGGTGAATTTGTCCTT 316 275 2325 Antisense
TTTGTCCTTCCCGATAATTGCAACC 124 667 2341 Antisense
GCAACCCATTCTATGCGATGGCTCC 297 321 2360 Antisense
CGACGTCGCCGATGTTAAACTTATT 518 203 2390 Antisense
GGGACCATTGAAGGTGGTGCCTCAC 121 495 2437 Antisense
Sequence Source Affymetrix Proprietary Database