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[192725_at] phorbol ester and DAG binding domain

Description F25B3.3 /REP_DB=WormBase Gene ID /WP=CE05713 /TR=Q19770 /GB=CAA94755.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=phorbol ester and DAG binding domain
Cluster 6grp_total > 34      4grp_total > 13      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 36.1
(0.8)
A 		9.4
(-2.3)
A 		3.2
(-3)
A 		55.3
(3)
P 		52
B 27.6
(1.7)
P 		16.6
(-0.6)
A 		5.5
(-3)
A 		33.5
(3)
A 		28
C 28.9
(-0.3)
P 		24.2
(-1.4)
A 		17.3
(-3)
A 		42.8
(3)
A 		26
avr 30.9
(0.8) 		16.7
(-1.6) 		8.7
(-3) 		43.9
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 37.4
(2.6)
M 		5
(-3)
A 		40
(3)
M 		13.3
(-1.6)
A 		10.8
(-2)
A 		13.5
(-1.5)
A 		35
B 23.7
(3)
A 		9.4
(-2.5)
A 		22.9
(2.7)
A 		11.2
(-1.8)
A 		8
(-3)
A 		21.9
(2.3)
A 		16
C 14.9
(-1)
A 		40
(3)
P 		5.1
(-2.5)
A 		2
(-3)
A 		4.5
(-2.6)
A 		10.4
(-1.7)
A 		38
avr 25.3
(3) 		18.1
(0.5) 		22.7
(2.1) 		8.8
(-2.7) 		7.8
(-3) 		15.3
(-0.4) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 13.1
(3)
A 		2
(-2.4)
A 		0.8
(-3)
A 		8.1
(0.6)
P 		12
B 8.8
(3)
P 		4.1
(-0.8)
A 		1.3
(-3)
A 		7.1
(1.6)
A 		8
C 9.6
(3)
P 		7
(-1.3)
A 		6
(-3)
A 		7.2
(-1)
A 		4
avr 10.5
(3) 		4.4
(-1.7) 		2.7
(-3) 		7.5
(0.7) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 6.9
(1.4)
M 		1.8
(-3)
A 		8.7
(3)
M 		3.4
(-1.6)
A 		3.3
(-1.7)
A 		3.3
(-1.7)
A 		7
B 8.3
(3)
A 		3.1
(-2.9)
A 		5.6
(-0.1)
A 		3
(-3)
A 		3.3
(-2.7)
A 		8.2
(2.9)
A 		5
C 5.5
(-0.4)
A 		12.2
(3)
P 		1.3
(-2.5)
A 		0.4
(-3)
A 		1.4
(-2.5)
A 		3.5
(-1.4)
A 		12
avr 6.9
(3) 		5.7
(1.4) 		5.2
(0.8) 		2.3
(-3) 		2.7
(-2.5) 		5
(0.5) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192725_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12096
Sequence Type Exemplar sequence
Representative Public ID F25B3.3 NCBI
Target Description F25B3.3 /REP_DB=WormBase Gene ID /WP=CE05713 /TR=Q19770 /GB=CAA94755.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=phorbol ester and DAG binding domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073068(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073068 NCBI Caenorhabditis elegans F25B3.3 (F25B3.3) mRNA, complete cds. 11/11 None
SNAP00000011931 ENSEMBL cdna:SNAP chromosome:CEL140:V:9560173:9561979:-1 9/11 None
GENEFINDER00000011941 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9559300:9564192:-1 11/11 None
F25B3.3 ENSEMBL cdna:known chromosome:CEL140:V:9559300:9564192:-1 gene:F25B3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9559299-9564192(-) 100.0 100.0
Public Domain and Genome References
Gene Title phorbol ester and DAG binding domain
Ensembl F25B3.3
Entrez Gene 179344 Entrez gene
SwissProt Q19770 EMBL-EBI
WormBase CE34003 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.9761.1.S1_AT similar to RAS guanyl releasing protein 3 (calcium and DAG-regulated) cfa
CHICKEN:GGA.1287.1.S1_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) gga
CHICKEN:GGAAFFX.12948.1.S1_S_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) gga
HG-U133_PLUS_2:205801_S_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U133A:205801_S_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U133A_2:205801_S_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HU35KSUBB:RC_AA002227_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HU35KSUBB:RC_R26124_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
U133_X3P:G7662333_3P_S_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U95AV2:34748_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U95C:52707_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
U133_X3P:HS.147800.0.A1_3P_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U133_PLUS_2:244526_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U133B:244526_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U95C:62375_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U95C:50697_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HG-U95C:51323_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
HU35KSUBA:N48010_AT RAS guanyl releasing protein 3 (calcium and DAG-regulated) hs
MOE430B:1438031_AT RAS, guanyl releasing protein 3 mm
MOUSE430_2:1438031_AT RAS, guanyl releasing protein 3 mm
MG-U74BV2:116602_AT RAS, guanyl releasing protein 3 mm
MG-U74BV2:116603_AT RAS, guanyl releasing protein 3 mm
MOE430B:1438030_AT RAS, guanyl releasing protein 3 mm
MOUSE430_2:1438030_AT RAS, guanyl releasing protein 3 mm
MG-U74CV2:134302_AT MKIAA0846 protein mm
MU19KSUBC:TC36600_AT MKIAA0846 protein mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5085 guanyl-nucleotide exchange factor activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94755 Hypothetical protein F25B3.3 [Caenorhabditis elegans] ref|NP_505469.3| F25B3.3 [Caenorhabditis elegans] 0.0
blast CAE72201 Hypothetical protein CBG19309 [Caenorhabditis briggsae] 0.0
blast CAE72201 Hypothetical protein CBG19309 [Caenorhabditis briggsae] 1.0E-171
blast XP_854079 PREDICTED: similar to RAS guanyl releasing protein 3 (calcium and DAG-regulated) [Canis familiaris] 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.9E-26
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.0E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.8E-4
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.2E-17
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.0E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.8E-4
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.2E-17
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.9E-26
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.0E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.8E-4
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.2E-17
Sequence
Target Sequence 
>C. ELEGANS:192725_AT
gtgggcctctggagttacagttgcacctgataatgcaacagtttcaaaacatatcagtgc
aatggtggacgcagtattcaaacactatgatcatgatagagatggattcatttctcaaga
agaatttcagttgattgctggaaatttcccttttattgacgcatttgtgaacatagatgt
tgatatggatggacagatatcaaaagatgaattgaagacctattttatggctgcaaacaa
gaatacaaaagatttacgaagaggattcaagcacaatttccatgaaactacattcttaac
tccaacgacttgcaatcattgcaacaaattgttgtgggggattttgagacaaggcttcaa
gtgcaaagattgtggattagcagttcatagttgctgtaaatcaaatgctgtagccgaatg
cagaagaaaaagtagcagcaatttaacaagggctgccgagtggttcgcttcaccacgtgg
cagtatgagaagtaggataataaatacatgcaataattccggaagtacaccagatgaaga
aattggtcttgtaagccttgcttgtgaagaagtcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGGCCTCTGGAGTTACAGTTGCA 304 487 1302 Antisense
GTGCAATGGTGGACGCAGTATTCAA 522 479 1358 Antisense
GAAATTTCCCTTTTATTGACGCATT 512 361 1442 Antisense
GACCTATTTTATGGCTGCAAACAAG 221 383 1518 Antisense
GAGGATTCAAGCACAATTTCCATGA 293 403 1562 Antisense
GAAACTACATTCTTAACTCCAACGA 670 355 1585 Antisense
AACTCCAACGACTTGCAATCATTGC 697 141 1599 Antisense
AACAAGGGCTGCCGAGTGGTTCGCT 392 133 1746 Antisense
TTCGCTTCACCACGTGGCAGTATGA 341 695 1765 Antisense
GAAATTGGTCTTGTAAGCCTTGCTT 250 363 1840 Antisense
AAGCCTTGCTTGTGAAGAAGTCTTC 342 149 1854 Antisense
Sequence Source Affymetrix Proprietary Database