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[192722_s_at] serine/threonine protein phosphatase /// protein phosphatase

Description F25B3.4 /REP_DB=WormBase Gene ID /WP=CE05714 /TR=Q27496 /GB=CAA94756.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine protein phosphatase
Cluster 6grp_total > 27      4grp_total > 20      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 83.1
(0.9)
P 		50.5
(-0.9)
A 		13.1
(-3)
A 		121.7
(3)
P 		109
B 57.1
(-2.3)
A 		73.4
(0.1)
A 		52.7
(-3)
A 		92.5
(3)
P 		40
C 88.5
(1.6)
M 		71.3
(-1.8)
A 		65.4
(-3)
P 		95.8
(3)
P 		30
avr 76.2
(0.3) 		65.1
(-0.8) 		43.7
(-3) 		103.3
(3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 203.8
(3)
P 		150.9
(1)
P 		43.8
(-3)
A 		55
(-2.6)
A 		77.5
(-1.7)
P 		96.3
(-1)
P 		160
B 148
(3)
P 		143.2
(2.6)
P 		69.7
(-3)
A 		84.9
(-1.8)
A 		87.3
(-1.7)
P 		85.2
(-1.8)
P 		78
C 176.7
(3)
P 		124.1
(-0.6)
P 		103.1
(-2.1)
P 		89.8
(-3)
A 		107.5
(-1.8)
P 		112.6
(-1.4)
P 		87
avr 176.2
(3) 		139.4
(0.9) 		72.2
(-3) 		76.6
(-2.7) 		90.8
(-1.9) 		98
(-1.5) 		104
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 30.3
(3)
P 		11
(-1.3)
A 		3.4
(-3)
A 		17.8
(0.2)
P 		27
B 18.2
(1.8)
A 		18.1
(1.7)
A 		12.2
(-3)
A 		19.7
(3)
P 		8
C 29.3
(3)
M 		20.5
(-1)
A 		22.8
(0)
P 		16.2
(-3)
P 		13
avr 25.9
(3) 		16.5
(-1.3) 		12.8
(-3) 		17.9
(-0.7) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 37.6
(0.8)
P 		53.7
(3)
P 		9.5
(-3)
A 		13.9
(-2.4)
A 		23.4
(-1.1)
P 		23.5
(-1.1)
P 		44
B 51.5
(3)
P 		47.7
(2.3)
P 		16.9
(-3)
A 		22.5
(-2)
A 		35.5
(0.2)
P 		31.8
(-0.4)
P 		35
C 65.5
(3)
P 		37.9
(-0.5)
P 		26.2
(-2)
P 		18.1
(-3)
A 		34.8
(-0.9)
P 		37.7
(-0.5)
P 		47
avr 51.5
(3) 		46.4
(2.1) 		17.5
(-3) 		18.2
(-2.9) 		31.2
(-0.6) 		31
(-0.6) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192722_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12215
Sequence Type Exemplar sequence
Representative Public ID F25B3.4 NCBI
Target Description F25B3.4 /REP_DB=WormBase Gene ID /WP=CE05714 /TR=Q27496 /GB=CAA94756.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine protein phosphatase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068375(11), NM_073069(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068375 NCBI Caenorhabditis elegans ZK354.9 (ZK354.9) mRNA, complete cds. 11/11 None
NM_073069 NCBI Caenorhabditis elegans F25B3.4 (F25B3.4) mRNA, complete cds. 11/11 A 
SNAP00000006010 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5306588:5308135:-1 11/11 None
GENEFINDER00000006017 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5304026:5307663:-1 11/11 None
SNAP00000011933 ENSEMBL cdna:SNAP chromosome:CEL140:V:9568212:9569293:-1 11/11 None
GENEFINDER00000011938 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9568212:9572875:-1 11/11 None
ZK354.9 ENSEMBL cdna:known chromosome:CEL140:IV:5306833:5308371:-1 gene:ZK354.9 11/11 None
F25B3.4 ENSEMBL cdna:known chromosome:CEL140:V:9568212:9570498:-1 gene:F25B3.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5306601-5307696(-) 90.41 90.41
chrV:9568211-9570498(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine protein phosphatase
protein phosphatase
Ensembl ZK354.9
F25B3.4
Entrez Gene 184915 Entrez gene
191296 Entrez gene
SwissProt Q27496 EMBL-EBI
P91569 EMBL-EBI
WormBase CE05714 Wormbase
CE15326 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42261 Hypothetical protein ZK354.9 [Caenorhabditis elegans] ref|NP_500776.1| ZK354.9 [Caenorhabditis elegans] 0.0
blast AAB42260 Hypothetical protein ZK354.8 [Caenorhabditis elegans] ref|NP_500775.1| ZK354.8 [Caenorhabditis elegans] 0.0
blast CAA94756 Hypothetical protein F25B3.4 [Caenorhabditis elegans] ref|NP_505470.1| F25B3.4 [Caenorhabditis elegans] 1.0E-171
blast CAA94756 Hypothetical protein F25B3.4 [Caenorhabditis elegans] ref|NP_505470.1| F25B3.4 [Caenorhabditis elegans] 1.0E-170
blast AAB42261 Hypothetical protein ZK354.9 [Caenorhabditis elegans] ref|NP_500776.1| ZK354.9 [Caenorhabditis elegans] 1.0E-158
blast CAE58977 Hypothetical protein CBG02250 [Caenorhabditis briggsae] 1.0E-157
blast CAA94757 Hypothetical protein F25B3.5 [Caenorhabditis elegans] ref|NP_505471.2| F25B3.5 [Caenorhabditis elegans] 1.0E-157
blast CAA94756 Hypothetical protein F25B3.4 [Caenorhabditis elegans] ref|NP_505470.1| F25B3.4 [Caenorhabditis elegans] 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 6.8E-38
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.4E-39
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 6.8E-38
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 1.5E-38
Pfam IPR000242 EMBL-EBI Tyrosine specific protein phosphatase 3.9E-98
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.4E-39
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.4E-39
Sequence
Target Sequence 
>C. ELEGANS:192722_S_AT
ttctattcgcctacaaagtcatctttccaaaccatttcttcatgttgcgaggcaatcacg
aatgttcacttatcaacaggcaatatggattctacgaagagtgtcagcgccgttacaaca
aaccatccgtatggcattcgtttcaaggtgtcttttcggttatgccattgacagctcttg
ttggtcagagaattttgtgtatgcatggcggtgtttccaaaatgcttcaaaacgtcagtc
aacttcgtgctatcaaacgtccatttgacaatcctgagccaaatacgcttgcaattgaca
ttctttggagcgatccaacaaacttccaaaaaggatggaacccgaactctcgtggtgttt
cgtacgtttttggatcagatgcccttcgcaaacttctcgatcgtcttcaaattgatctcg
tggttcgtgctcatcaagttgtgcaagacggctacgaattctttgctaatcgtcgtctgg
ttacaatcttttcggcaccgttctactgtggacagttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTATTCGCCTACAAAGTCATCTT 299 691 260 Antisense
TCATCTTTCCAAACCATTTCTTCAT 50 621 278 Antisense
GCGAGGCAATCACGAATGTTCACTT 212 289 306 Antisense
ATGCCATTGACAGCTCTTGTTGGTC 18 41 421 Antisense
GTCAGTCAACTTCGTGCTATCAAAC 296 467 493 Antisense
ACGTCCATTTGACAATCCTGAGCCA 688 87 516 Antisense
AAACTTCTCGATCGTCTTCAAATTG 626 127 649 Antisense
GACGGCTACGAATTCTTTGCTAATC 157 377 706 Antisense
TTTGCTAATCGTCGTCTGGTTACAA 481 667 721 Antisense
TGGTTACAATCTTTTCGGCACCGTT 416 551 737 Antisense
GGCACCGTTCTACTGTGGACAGTTT 64 525 753 Antisense
Sequence Source Affymetrix Proprietary Database